Characterization and detection ofCucumber green mottle mosaic virus in Greece

Varveri, C.; Vassilakos, Nikon; Bem, F.

doi:10.1007/bf02979754

Cited by 60 publications

(41 citation statements)

References 12 publications

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“…The tobamovirus, CGMMV causes severe disease of cucurbits worldwide. The typical symptoms in watermelon including leaf mottling and mosaic have been reported in greenhouse-grown plants (Varveri et al, 2002;Shim et al, 2005), but these symptoms are masked in plants growing in open fields (Reingold et al, 2013). Serological testing by ELISA of a large number of fruits exhibiting systemic mosaic and mild to severe yellow mottling symptoms, confirmed the presence of CGMMV.…”

Section: Discussionmentioning

confidence: 76%

“…CGMMV has been reported from few Mediterranean countries: France, Greece, Israel, Spain, and Syria (Avgelis and Vovlas, 1986;Antignus et al, 1990;Blancard et al, 1994;Celix et al, 1996;Varveri et al, 2002;Kassem et al, 2005). In Saudi Arabia, a severe infection outbreak was observed on watermelon and bottle gourd crops (AlShahwan and Abdalla, 1992; Al-Saleh and Al- Shahwan, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Total RNA was extracted from infected and uninfected bottle gourd and watermelon leaf samples using ISOLATE Plant RNA Mini Kit (BioLine Ltd, London, United Kingdom). The oligonucleotide primer (105 and 106) and RT-PCR condition were performed as described by (Varveri et al, 2002) using one step RT-PCR kit (Qiagen) in a Thermal cycler, Mastercycler® None gradient (Eppendorf, Germany). RT-PCR products were analyzed by electrophoresis in 1% agarose gel, visualized by ethidium bromide staining and 50 bp DNA ladder (Cat No.…”

Section: Reverse Transcription-polymerase Chain Reaction (Rt-pcr) Detmentioning

confidence: 99%

“…CGMMV was originally described in the UK 1935 (Ainsworth, 1935). Thereafter reports were made in USA and Canada (Tian et al, 2014;Ling et al, 2014); Saudi Arabia (Al-Shahwan and Abdalla, 1992; Al-Saleh and Al- Shahwan, 1997); Iran (Moradi and Jafarpour, 2011), China and Greece (Zhang et al, 2009;Varveri et al, 2002), Korea and Myammar (Yoon et al, 2008;Kim et al, 2010), and in the Ukraine (Budzanivska et al, 2007). Several strains of CGMMV have been reported from Europe, Pakistan, India and Japan (Komuru et al, 1968; Lee, 1996;Antignus et al, 1990;Francki et al, 1986;Ugaki et al, 1991;Shim et al, 2005;Ko et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

Amer¹

2015

IJAB

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To cite this paper: Amer, M.A., 2015. Biological and molecular characterization of cucumber green mottle mosaic virus affecting bottle gourd and watermelon plants in Saudi Arabia. AbstractSamples were collected from seven of each bottle gourd and watermelon plants grown in Riyadh and Hail regions of Saudi Arabia in May, 2012 showing symptom suspected to be virus infection and analyzed by a double antibody sandwich against bottle gourd and watermelon viruses. Two of watermelon and three of bottle gourd samples were found to be positive with Cucumber green mottle mosaic virus (CGMMV). However, variable results were obtained with other viruses infecting cucurbits. One sample of CGMMV positive samples from each region were homogenized in a mortar separately and used to make mechanical inoculation on 25 plant species which produced characteristic symptoms of tobamo virus infection. Five ELISA-positive samples from naturally infection were further tested and detected by RT-PCR assay using virus specific primers that amplified a 400 bp fragment in the coat protein gene. The nucleotide sequence of the PCR products from the Saudi isolates of CGMMV isolated from bottle gourd and watermelon were determined and demonstrated its similarity to the other isolates of CGMMV recorded in NCBI. The CP gene of CGMMV-Saudi Arabia isolates shared between 87.2% and 99.7% identity in nucleotide level. The synthesized cDNA probe for CGMMV detection was hybridized with nucleic acid extracts from infected samples collected from the above mentioned locations. This is the first report regarding the genetic makeup of CGMMV at the molecular level in Saudi Arabia.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Reverse Transcription-polymerase Chain Reaction (Rt-pcr) Detmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

Amer¹

2015

IJAB

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“…It causes systemic mosaic and mottling on leaves, blistering, deterioration of fruit pulp, and dwarfism, resulting in major economic losses. CGMMV has a 6.4 kb (+)ssRNA genome and rodshaped virions, approximately 300 nm in length and 18 nm in diameter (Varveric et al 2006). Similar to other viruses, the CGMMV virion replicates itself by entering a host cell, disassembling itself and copying its components.…”

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confidence: 99%

Inhibitory effect of gamma irradiation against Cucumber green mottle mosaic virus

Jin-Sung¹,

Jeong²,

Rae-Dong³

2017

Plant Prot. Sci.

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Gamma irradiation has been shown to be an effective method of controlling plant bacterial and fungal pathogens, but data on its effect against plant viruses is limited. A mechanism for the inactivation of plant viruses by gamma irradiation has not been proposed. Gamma irradiation was evaluated for the inactivation of Cucumber green mottle mosaic virus (CGMMV) in nicotiana tabacum plants. CGMMV infectivity decreased gradually in a dose-dependent manner, and the virus was completely inactivated at over 40 kGy. Transmission electron microscopy revealed that gamma irradiation disrupts the virion structure and degrades viral protein and genomic RNA, suggesting that damage to viral constituents is the mechanism by which gamma irradiation inactivates the virus.

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