Characterization and degradation of functionalized chitosan with glycidyl methacrylate

Flores-Ramírez, Nelly; Elizalde-Peña, Eduardo A.; Vásquez-García, S. R.; González‐Hernández, J.; Martínez-Ruvalcaba, A.; Sánchez, Isaac C.; Luna‐Bárcenas, Gabriel; Gupta, Ram B.

doi:10.1163/1568562053700174

Cited by 53 publications

(34 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, we explored the development of multivalent sialic acid containing materials through different synthesis routes. Many other investigators have used glycidyl methacrylate derivatized polysaccharides for the preparing biocompatible hydrogels [37][38][39][40][41][42], thus we explored the feasibility of using glycidyl methacrylate sialic acid oligosaccharides for preparing biocompatible soluble (low molecular weight) polymers for use in Aβ toxicity attenuation applications that might be useful in Alzheimer's disease.…”

Section: Discussionmentioning

confidence: 99%

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

2008

View full text Add to dashboard Cite

Abstractβ-Amyloid peptide (Aβ), the primary protein component in senile plaques associated with Alzheimer's disease (AD), has been implicated in neurotoxicity associated with AD. Previous studies have shown that the Aβ-neuronal membrane interaction plays a crucial role in Aβ toxicity. More specifically, it is thought that Aβ interacts with ganglioside rich and sialic acid rich regions of cell surfaces. In light of such evidence, we have hypothesized that the Aβ-membrane sialic acid interaction could be inhibited through use of a biomimic multivalent sialic acid compound that would compete with the cell surface for Aβ binding. To explore this hypothesis, we synthesized a series of photocrosslinked sialic acid containing oligosaccharides and tested their ability to bind Aβ and attenuate Aβ toxicity in cell culture assays. We show that a polymer prepared via the photocrosslinking of disialyllacto-N-tetraose (DSLNT) was able to attenuate Aβ toxicity at low micromolar concentrations without adversely affecting the cell viability. Polymers prepared from mono-sialyl-oligosaccharides were less effective at Aβ toxicity attenuation. These results demonstrate the feasibility of using photocrosslinked sialyl-oligosaccharides for prevention of Aβ toxicity in vitro and may provide insight into the design of new materials for use in attenuation of Aβ toxicity associated with AD.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

2008

View full text Add to dashboard Cite

show abstract

“…The spectra of CTS-g-GMA and (CTS-g-GMA)-PEGDA demonstrated some changes when compared to the pure chitosan, as observed in Figure 1 and Figure 2. For the CTS-g-GMA spectrum, the peaks at 1557 cm -1 and 1635 cm -1 correspond to C=C stretching and C=O vibration indicating the success of grafting of glycidyl methacrylate onto the chitosan structure [4,19,22] For the (CTS-g-GMA)-PEGDA spectrum, the band at 1732 cm −1 clearly showed the presence of the carbonyl group of PEGDA owing to photopolymerization of CTS-g-GMA and PEGDA for the fabrication of chitosan-based hydrogel [23]. It is also notable that the band 1557 cm -1 corresponding to C=C stretching disappears due to the insertion of the acrylate groups of PEGDA to CTS-g-GMA during photopolymerization process in (CTS-g-GMA)-PEGDA.…”

Section: Characterization Of Chitosan-based Hydrogel By Photopolymerimentioning

confidence: 99%

Synthesis of chitosan-based hydrogel by using photopolymerization technique

Alemdar

2016

AUBTD-A

View full text Add to dashboard Cite

In this study, a novel route was developed to fabricate chitosan-based hydrogel by using photopolymerization technique. Firstly, glycidyl methacrylate (GMA) grafted onto the chitosan (CTS) backbone to produce chitosan derivative product having photopolymerizable groups (CTS-g-GMA). Following, CTS-g-GMA and PEGDA are subjected to photopolymerization to form chemically crosslinked hydrogel. The chemical structure of the obtained chitosan-based hydrogel was characterized by Fourier transform infrared (FT-IR) analyses. Thermal behavior of hydrogel was determined by thermal gravimetric analysis (TGA) and differential thermal analysis (DTA). The thermal analyses showed that (CTS-g-GMA)-PEGDA had higher thermal stability than neat chitosan. Water uptake capacities (swelling ratio) of hydrogels obtained by using different UV exposure time were determined gravimetrically. From the swelling test result, it was observed that exposure time has the effect on the water uptake capacity of hydrogels. Additionally, cytotoxicity test for chitosan-based hydrogel was carried out by using L929 fibroblasts. All of the obtained results demonstrated that chitosan-based hydrogel fabricated in this work could be used for biomedical applications in future works.

show abstract

“…The peaks observed at 2960 cm −1 and 2920 cm −1 demonstrate the asymmetric and symmetric stretching vibrations of CH groups in CTS . CO stretching (Amide I) and NH bending (Amide II) vibrations of amide groups in CTS are confirmed by the peaks at 1660 and 1600 cm −1 . The intense peak appearing at 1050 cm −1 is due to stretching vibrations of ether functionality of CTS .…”

Section: Resultsmentioning

confidence: 81%

Production of reduced graphene oxide‐based electrically conductive hydrogel by using modified chitosan

Ulutürk

Alemdar

2019

J of Applied Polymer Sci

View full text Add to dashboard Cite

Herein, a new approach was applied to produce reduced graphene oxide (RGO)‐based conductive hydrogel by using modified chitosan (CTS) as a primary constituent. A variety amounts of RGO (from 0 to 15%) were incorporated into the polymeric network generated by photopolymerization of CTS‐graft‐glycidyl methacrylate (CTS‐g‐GMA) and poly(ethylene glycol)diacrylate (PEGDA). The structures of hydrogels were confirmed by FT‐IR, XRD, and SEM analyses. Water uptake capacity of hydrogels determined gravimetrically. L929 fibroblast cells were used for cytotoxicity test. According to conductivity measurements carried out by four‐point probe technique, the highest conductivity (1.716 × 10−3 S/cm) was obtained when 10% RGO was encapsulated into the polymeric structure. From the results, it could be envisaged that electroconductive hydrogel (ECH) fabricated in this study could have a potential usage for biosensor applications in the future projects. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 48008.

show abstract

Characterization and degradation of functionalized chitosan with glycidyl methacrylate

Cited by 53 publications

References 35 publications

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

Synthesis of chitosan-based hydrogel by using photopolymerization technique

Production of reduced graphene oxide‐based electrically conductive hydrogel by using modified chitosan

Contact Info

Product

Resources

About