Characterization and cytocompatibility of carbon layers prepared by photo-induced chemical vapor deposition

Kubova, Olga; Švorčı́k, Václav; Heitz, J.; Moritz, Sieglinde; Romanin, Christoph; Matějka, Pavel; Macková, Anna

doi:10.1016/j.tsf.2007.02.014

Cited by 30 publications

(31 citation statements)

References 27 publications

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“…Thin carbon layers are considered as a prospective material for a wide range of biomedical application [39,[43][44][45], e.g. tissue regeneration [46], controlled drug delivery [47], surface coating for bone-related implants [48], increase of resistance to microbial adherence, blood interfacing implants applications [49] or neuronal growth.…”

Section: Carbon Nanolayersmentioning

confidence: 99%

“…The preparation of the carbon layers on polytetrafluoroethylene (PTFE) by photoinduced CVD from acetylene and their physical properties and chemical structure have been studied. These properties related to the adhesion and proliferation of human umbilical endothelial cells (HUVEC) seeded thereon were characterized [39].…”

Section: Chemical Vapour Depositionmentioning

confidence: 99%

“…The surface roughness is decreasing after more than 20 min deposition [39]. The thickness of the deposited layers was measured by SEM microscopy and profilometry [39]. For both methods silicon platelets were used as substrates.…”

Section: Surface Morphology and Layer Thicknessmentioning

confidence: 99%

“…The amount of cells was determined by counting from images and MTS test [39]. The positive influence of the photo-deposited carbon layer for cytocompatibility is more significant after 3 and even more after 7 days of proliferation.…”

Section: Cell-surface Interactionmentioning

confidence: 99%

“…Sputtered or evaporated [37] carbon structures can create nanostructures of different electrical or morphological properties. The opportunities for systematic investigation of nanolayers structures [38][39][40][41][42] are very promising for new application both in electronics or nanoengineering and biomedicine.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The Properties and Application of Carbon Nanostructures

Slepička¹,

Hubáček²,

Kolská³

et al. 2013

Polymer Science

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Section: Carbon Nanolayersmentioning

confidence: 99%

Section: Chemical Vapour Depositionmentioning

confidence: 99%

Section: Surface Morphology and Layer Thicknessmentioning

confidence: 99%

Section: Cell-surface Interactionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Properties and Application of Carbon Nanostructures

Slepička¹,

Hubáček²,

Kolská³

et al. 2013

Polymer Science

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Bioactivity and hemocompatibility study of amorphous hydrogenated carbon coatings produced by pulsed magnetron discharge

López‐Santos

Colaux

Laloy

et al. 2012

J Biomedical Materials Res

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Literature contains very few data about the potential biomedical application of amorphous hydrogenated carbon (a-C:H) thin films deposited by reactive pulsed magnetron discharge even so it is one of the most scalable plasma deposition technique. In this article, we show that such a C2H2 pulsed magnetron plasma produces high quality coating with good hemocompatibility and bioactive response: no effect on hemolysis and hemostasis were observed, and proliferation of various cell types such as endothelial, fibroblast, and osteoblast-like cells was not affected when the deposition conditions were varied. Cell growth on a-C:H coatings is proposed to take place by a two-step process: the initial cell contact is affected by the smooth topography of the a-C:H coatings, whereas the polymeric-like structure, together with a moderate hydrophilicity and a high hydrogen content, directs the posterior cell spreading while preserving the hemocompatible behavior.

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In vitro cytocompatibility assessment of amorphous carbon structures using neuroblastoma and Schwann cells

2013

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The development of scaffolds for neural tissue engineering application requires an understanding of cell adhesion, proliferation, and migration of neuronal cells. Considering the potential application of carbon as scaffold materials and the lack of understanding of compatibility of amorphous carbon with neuronal cells, the carbon-based materials in the forms of carbon films and continuous electrospun carbon nanofibers having average diameter of ~200 nm are being investigated with or without ultraviolet (UV) and oxy-plasma (OP) treatments for cytocompatibility property using mouse Neuroblastoma (N2a) and rat Schwann cells (RT4-D6P2T). The use of Raman spectroscopy in combination with Fourier transform infrared (FTIR) and X-ray diffraction establishes the amorphous nature and surface-bonding characteristics of the studied carbon materials. Although both UV and OP treatments make carbon surfaces more hydrophilic, the cell viability of N2a cells is statistically more significant on OP treated fibers/films compared to UV fiber/film substrates after 4 days in culture. The electrospun carbon fibrous substrate provides the physical guidance to the cultured Schwann cells. Overall, the experimental results of this study demonstrate that the electrospun amorphous carbon nanofibrous scaffolds can be used as a suitable biomaterial substrate for supporting cell adhesion and proliferation of neuronal cells in the context of their applications as artificial nerve implants.

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Characterization and cytocompatibility of carbon layers prepared by photo-induced chemical vapor deposition

Cited by 30 publications

References 27 publications

The Properties and Application of Carbon Nanostructures

The Properties and Application of Carbon Nanostructures

Bioactivity and hemocompatibility study of amorphous hydrogenated carbon coatings produced by pulsed magnetron discharge

In vitro cytocompatibility assessment of amorphous carbon structures using neuroblastoma and Schwann cells

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