Characterization and Control of Pestalotiopsis spp. the Causal Fungus of Guava Scabby Canker in El-Beheira Governorate, Egypt

El-Argawy, Eman

doi:10.33687/phytopath.004.03.1403

Cited by 6 publications

(9 citation statements)

References 33 publications

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“…The remaining 34 isolates had whitish cream mycelium with black fruiting structure, acervuli on the upper side, and had light orange to orange at lower side. These isolates produced spores having 3-4 septa and 2 or 3 appendages, characteristics of Pestalotiopsis microspora as reported by El-argawy [ 33 ]. Further molecular characteristic of these isolates using universal primers ITS1 and ITS4 confirmed the species as Pestalotiopsis microspora as discussed below in molecular characterisation section.…”

Section: Discussionsupporting

confidence: 64%

Morphological and Molecular Identification of the Causal Agent of Anthracnose Disease of Avocado in Kenya

Kimaru

Monda

Cheruiyot

et al. 2018

International Journal of Microbiology

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Anthracnose disease of avocado contributes to a huge loss of avocado fruits due to postharvest rot in Kenya. The causal agent of this disease has not been clear but presumed to be Colletotrichum gloeosporioides as reported in other regions where avocado is grown. The fungus mainly infects fruits causing symptoms such as small blackish spots, “pepper spots,” and black spots with raised margin which coalesce as infection progresses. Due to economic losses associated with the disease and emerging information of other species of fungi as causal agents of the disease, this study was aimed at identifying causal agent(s) of the disease. A total of 80 fungal isolates were collected from diseased avocado fruits in Murang'a County, the main avocado growing region in Kenya. Forty-six isolates were morphologically identified as Colletotrichum spp. based on their cultural characteristics, mainly whitish, greyish, and creamish colour and cottony/velvety mycelia on the top side of the culture and greyish cream with concentric zonation on the reverse side. Their spores were straight with rounded end and nonseptate. Thirty-four isolates were identified as Pestalotiopsis spp. based on their cultural characteristics: whitish grey mycelium with black fruiting structure on the upper side and greyish black one on the lower side and septate spores with 3-4 septa and 2 or 3 appendages at one end. Further molecular studies using ITS indicated Colletotrichum gloeosporioides, Colletotrichum boninense, and Pestalotiopsis microspora as the causal agents of anthracnose disease in avocado. However, with this being the first report, there is a need to conduct further studies to establish whether there is coinfection or any interaction thereof.

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Section: Discussionsupporting

confidence: 64%

Morphological and Molecular Identification of the Causal Agent of Anthracnose Disease of Avocado in Kenya

Kimaru

Monda

Cheruiyot

et al. 2018

International Journal of Microbiology

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“…However, other species of Pestalotiopsis have also been evaluated in such a way by other authors in eucalyptus crops [20]. According to [21], the species Pestalotiopsis psidii, P. microspora, P. neglecta and P. clavispora, caused lesions on leaves of guava (Psidium guajava L.), a tropical fruit plant, with similar characteristics in comparison to those presented on the present study, mainly regarding the size of the lesions, which varied between 18.2 and 36.4 mm 2 . In a similar study, [22] identified P. microspora occurring in the ornamental plant Hypericum patulum, causing severe lesions in the leaf area, but with a lesion area of greater extent than in the present study, from 20 mm 2 reaching 113 mm 2 in size.…”

Section: Resultsmentioning

confidence: 99%

Re-Isolation Methodologies for Recovering Sporulation of Eucalyptus Pestalotiopsis grandis-urophylla Isolates after 14 Months Storage

Silva

Marques

Muniz

et al. 2021

JSRR

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After long periods of storage, plant pathogen isolates lose their sporulation capacity. The objective of this study was to evaluate re-isolation methodologies for recovering sporulation of Pestalotiopsis grandis-urophylla isolates after subjection to a long period of storage. Isolates of P. grandis-urophylla were kept for 14 months on Petri dishes with PDA medium at 10°C. After this period, the isolate colonies showed reduced mycelial growth and no sporulation. The isolates were inoculated on healthy Eucalyptus grandis-urophylla leaves, and after ten days they were subjected to three re‑isolation methods: scraping of the lesions (S) removing of injured plant tissue fragments, followed by disinfestation (D) and without disinfestation (WD). Then, the purified isolates were evaluated for the recovery of its sporulation ability. The different methods for re-isolation resulted in the occurrence of differences among the isolates, showing that sporulation is an isolate-dependent feature. The three methods (S, WD and D) allowed the sporulation recovery of P. grandis-urophylla, even after these isolates have been subjected to 14 months.

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“…Using the above criteria, the Panel identified the following plant species (crops and ornamentals) as main hosts of P. microspora relevant for the EU: Actinidia chinensis (Li et al., 2016 ), Ampelopsis grossedentata (Yuan et al., 2022 ), Eriobotrya japonica (Lu et al., 2016 ), Musa spp. (Bhuiyan et al., 2022 ), Persea americana (Kimaru et al., 2018 ), Psidium guajava (El‐Argawy, 2015 ) and Vaccinium corymbosum (Yi‐Lan et al., 2021 ).…”

Section: Pest Categorisationmentioning

confidence: 99%

“…Scabby fruit canker, caused by P. psidii, P. microspora, P. clavispora , P. neglecta and Pestalotiopsis sp. is one of the most common fruit diseases of guava ( Psidium guajava ), as it affects all the developmental stages of the fruit (El‐Argawy, 2015 ; Kwee & Chong, 1990 ). The disease can drastically reduce fruit yield during the preharvest stage, but it can also result in great losses during fruit storage.…”

Section: Pest Categorisationmentioning

confidence: 99%

Pest categorisation of Pestalotiopsis microspora

Bragard,

Baptista,

Chatzivassiliou

et al. 2023

EFS2

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Following an EFSA commodity risk assessment of bonsai plants (Pinus parviflora grafted on Pinus thunbergii) imported from China, the EFSA Plant Health Panel performed a pest categorisation of Pestalotiopsis microspora, a clearly defined plant pathogenic fungus of the family Pestalotiopsidaceae. The pathogen was reported on a wide range of monocotyledonous, dicotyledonous and gymnosperms, either cultivated or wild plant species, causing various symptoms such as leaf spot, leaf blight, scabby canker, fruit spot, pre-and post-harvest fruit rot and root rot. In addition, the fungus was reported as an endophyte on a wide range of asymptomatic plant species. This pest categorisation focuses on the hosts that are relevant for the EU and for which there is robust evidence that the pathogen was formally identified by a combination of morphology, pathogenicity and multilocus sequencing analyses. Pestalotiopsis microspora was reported in Africa, North, Central and South America, Asia and Oceania. In the EU, it was reported in the Netherlands. There is a key uncertainty on the geographical distribution of P. microspora worldwide and in the EU, because of the endophytic nature of the fungus, the lack of surveys, and because in the past, when molecular tools were not fully developed, the pathogen might have been misidentified as other Pestalotiopsis species or other members of the Pestalodiopsidaceae family based on morphology and pathogenicity tests. Pestalotiopsis microspora is not included in Commission Implementing Regulation (EU) 2019/2072. Plants for planting, fresh fruits, bark and wood of host plants as well as soil and other growing media associated with plant debris are the main pathways for the entry of the pathogen into the EU. Host availability and climate suitability in parts of the EU are favourable for the establishment and spread of the pathogen. The introduction and spread of the pathogen into the EU are expected to have an economic and environmental impact where susceptible hosts are grown. Phytosanitary measures are available to prevent the introduction and spread of the pathogen into the EU. Unless the restricted distribution in the EU is disproven, Pestalotiopsis microspora satisfies all the criteria that are within the remit of EFSA to assess for this species to be regarded as potential Union quarantine pest.

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Characterization and Control of Pestalotiopsis spp. the Causal Fungus of Guava Scabby Canker in El-Beheira Governorate, Egypt

Cited by 6 publications

References 33 publications

Morphological and Molecular Identification of the Causal Agent of Anthracnose Disease of Avocado in Kenya

Morphological and Molecular Identification of the Causal Agent of Anthracnose Disease of Avocado in Kenya

Re-Isolation Methodologies for Recovering Sporulation of Eucalyptus Pestalotiopsis grandis-urophylla Isolates after 14 Months Storage

Pest categorisation of Pestalotiopsis microspora

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