Characterization and complete genome sequence of the Shigella bacteriophage pSf-1

Complete Genome Sequence of <b><i>Proteus mirabilis</i></b> Phage pPM_01 Isolated from Raw Sewage

“…Phage specificity was examined using a spot test assay against several bacterial strains (Table 1) on LB agar plates as described by Jun et al [35], with modifications. In each test, a volume of 100 µL (OD 600 ∼0.5) of bacteria culture and 3 mL of molten soft agar were added to the base plate.…”

Bacteriophage Genome Sequencing: A New Alternative to Understand Biochemical Interactions between Prokaryotic Cells and Phages

“…As shown, phage genetics vary among phages of different hosts, even in an interlinked supply chain like the industry of food processing [24][25][26][27][28][29]. The main bottleneck for functional overall genomics, including phage genomics, is the low number of available genomes and described genes (open reading frames or ORFs).…”

Characterization and complete genome sequence of a novel N4-like bacteriophage, pSb-1 infecting Shigella boydii

“…The stability tests of pSb-1 were carried out as previously described [20]. Briefly, the stability of the phage at various temperatures (4,20,25,30,37,50, and 65 C) was assessed by incubating the phage at the respective temperatures for 1 h. To evaluate the stability of the phage at different pHs (3, 5, 7, 9, and 11), phage suspensions were incubated at 25 C for 1 h. After incubation, the phage titer was estimated by the double-layer agar method.…”

“…Phage structural proteins were analyzed by SDS-PAGE as previously described [20]. After electrophoresis, the protein bands were visualized by staining the gel with Coomassie blue R-250 and identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) at the National Instrumentation Center for Environmental Management (NICEM) at Seoul National University.…”

“…It is known that the isolation rate of S. boydii is less than 1e2% of all Shigella isolates [40]. Although antibiotic therapy is considered an effective treatment for shigellosis, the imprudent use of antibiotics has led to the increase of multiple-antibiotic-resistant Shigella species globally [5,20,26,33,36,39]. Increasingly, there have been more reports of antibiotic resistance in S. boydii strains.…”