Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

Abstract

Background: Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry. Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains. Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles. Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF. Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.

Assessment of animal diseases caused by bacteria resistant to antimicrobials: Swine

“…For beta-lactams, 9 of 11 studies reported less than 2% of isolates resistant to ceftiofur. Cid et al ( 2019) and Bessone et al (2019) showed slightly higher proportions, namely 8.7% and 7% in Spain and Argentina, respectively. For the aminopenicillins, Cid et al ( 2019) also found a high proportion of ampicillin resistance (33.3%).…”

“…Nine out of 13 studies found less than 8% of isolates resistant to florfenicol . The exceptions included three Asian studies reporting 16.3–91.6% resistance (Lee et al., 2012 ; Yeh et al., 2017 ; Kim et al., 2019 ) and one Argentinian study (Bessone et al., 2019 ) with 20% resistance. So, the trend of high florfenicol resistance levels in Asia (see previous sections on S. suis and A. pleuropneumoniae ) is confirmed for this bacterial species also.…”

“…(2019) and Bessone et al. (2019) showed slightly higher proportions, namely 8.7% and 7% in Spain and Argentina, respectively. For the aminopenicillins , Cid et al.…”

Effect of conditioned media from Aeromonas caviae on the transcriptomic changes of the porcine isolates of Pasteurella multocida

“…3 Department of Zoology, Faculty of Science, Kasetsart University, 10900 Bangkok, Thailand. 4 Department of Genetics, Faculty of Science, Kasetsart University, 10900 Bangkok, Thailand.…”

“…Pasteurella multocida is a Gram-negative bacterium that could be isolated from respiratory tracts of both healthy and diseased animals [1][2][3][4]. It is an opportunistic pathogen that causes economically significant respiratory diseases in pigs, including pneumonic pasteurellosis, atrophic rhinitis, and porcine respiratory disease complex (PRDC) [2,[5][6][7].…”