Characterization and comparison of putative<i>Stenotrophomonas maltophilia</i>methyl parathion hydrolases

Iyer, Rupa; Iken, Brian; Leon, Alex

doi:10.1080/10889868.2015.1114462

Cited by 8 publications

(10 citation statements)

References 35 publications

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“…Stenotrophomonas are ubiquitous bacteria, occupying various habitats, including harsh environments (Ryan et al, 2009;Hughes et al, 2016). They can use a wide range of substances for their growth (Juhasz, Stanley & Britz, 2000;Pages et al, 2008;Zhang et al, 2009;Urszula et al, 2009;Iyer, Iken & Leon, 2016). The vast metabolic capability of Stenotrophomonas species has encouraged various studies which aimed at find new paths for their biotechnological application, such as bioremediation, biodegradation, plant growth promotion, removal of organophosphate and synthesis of new antimicrobial agents (Ryan et al, 2009;Rajkumar et al, 2010;Iyer, Iken & Leon, 2016;Arulazhagan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The Polycyclic Aromatic Hydrocarbon (PAH) degradation activities and genome analysis of a novel strainStenotrophomonas sp. Pemsol isolated from Mexico

Elufisan

Rodríguez-Luna

Oyedara

et al. 2020

PeerJ

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Background Stenotrophomonas are ubiquitous gram-negative bacteria, which can survive in a wide range of environments. They can use many substances for their growth and are known to be intrinsically resistant to many antimicrobial agents. They have been tested for biotechnological applications, bioremediation, and production of antimicrobial agents. Method Stenotrophomonas sp. Pemsol was isolated from a crude oil contaminated soil. The capability of this isolate to tolerate and degrade polycyclic aromatic hydrocarbons (PAH) such as anthraquinone, biphenyl, naphthalene, phenanthrene, phenanthridine, and xylene was evaluated in Bushnell Hass medium containing PAHs as the sole carbon sources. The metabolites formed after 30-day degradation of naphthalene by Pemsol were analyzed using Fourier Transform Infra-red Spectroscopic (FTIR), Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). The genome of Pemsol was also sequenced and analyzed. Results Anthraquinone, biphenyl, naphthalene, phenanthrene, and phenanthridine except xylene can be used as sole carbon sources for Pemsol’s growth in Bushnell Hass medium. The degradation of naphthalene at a concentration of 1 mg/mL within 30 days was tested. A newly formed catechol peak and the disappearance of naphthalene peak detected on the UPLC-MS, and GC-MS analyses spectra respectively confirmed the complete degradation of naphthalene. Pemsol does not produce biosurfactant and neither bio-emulsify PAHs. The whole genome was sequenced and assembled into one scaffold with a length of 4,373,402 bp. A total of 145 genes involved in the degradation of PAHs were found in its genome, some of which are Pemsol-specific as compared with other 11 Stenotrophomonas genomes. Most specific genes are located on the genomic islands. Stenotrophomonas sp. Pemsol’s possession of few genes that are associated with bio-emulsification gives the genetic basis for its inability to bio-emulsify PAH. A possible degradation pathway for naphthalene in Pemsol was proposed following the analysis of Pemsol’s genome. ANI and GGDH analysis indicated that Pemsol is likely a new species of Stenotrophomonas. It is the first report on a complete genome sequence analysis of a PAH-degrading Stenotrophomonas. Stenotrophomonas sp. Pemsol possesses features that make it a good bacterium for genetic engineering and will be an excellent tool for the remediation of crude oil or PAH-contaminated soil.

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Section: Introductionmentioning

confidence: 99%

The Polycyclic Aromatic Hydrocarbon (PAH) degradation activities and genome analysis of a novel strainStenotrophomonas sp. Pemsol isolated from Mexico

Elufisan

Rodríguez-Luna

Oyedara

et al. 2020

PeerJ

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“…It showed two growth peaks in the mix, indicating that this strain preferred to use some compounds as sole carbon source rather than others. Previous studies have reported that Stenotrophomonas possess the ability to degrade xenobiotic, PAHs and organophosphates (Ryan et al, 2009;Iyer, Iken & Leon, 2016). S. maltophilia strains with such capabilities have been isolated from different environments ranging from common environment to extreme environment such as highly acidic or basic environment (Felsenstein, 1985;Boonchan, Britz & Stanley, 1998;Juhasz, Stanley & Britz, 2000;Samanta, Singh & Jain, 2002;Gao et al, 2013;Tebyanian, Hassanshahian & Kariminik, 2013;Arulazhagan et al, 2017b).…”

Section: Utilization Of Pahs By Stenotrophomonas Sp Pemsol As Sole Cmentioning

confidence: 99%

“…A number of genomes of strains belonging to the genus Stenotrophomonas have been sequenced, including clinical isolates (Lira et al, 2012;Iyer, Iken & Leon, 2016), but to date no genome with emphasis on the strain with PAH degradation was reported. The genome of Stenotrophomonas sp.…”

Section: Genetic Basis For the Degradation Of Pah By Stenotrophomonasmentioning

confidence: 99%

“…Stenotrophomonas species are ubiquitous bacteria, occupying various habitats including harsh environment (Ryan et al, 2009;Hughes et al, 2016). They can use a wide range of substances for their growth (Juhasz, Stanley & Britz, 2000;Pages et al, 2008;Zhang et al, 2009;Urszula et al, 2009;Iyer, Iken & Leon, 2016). The vast metabolic capability of Stenotrophomonas species has encouraged the studies to aim at finding new paths for their biotechnological application, such as bioremediation, biodegradation, plant growth promotion, removal of organophosphate and synthesis of antimicrobial agents (Ryan et al, 2009;Rajkumar et al, 2010;Iyer, Iken & Leon, 2016;Arulazhagan et al, 2017a).…”

Section: Introductionmentioning

confidence: 99%

“…They can use a wide range of substances for their growth (Juhasz, Stanley & Britz, 2000;Pages et al, 2008;Zhang et al, 2009;Urszula et al, 2009;Iyer, Iken & Leon, 2016). The vast metabolic capability of Stenotrophomonas species has encouraged the studies to aim at finding new paths for their biotechnological application, such as bioremediation, biodegradation, plant growth promotion, removal of organophosphate and synthesis of antimicrobial agents (Ryan et al, 2009;Rajkumar et al, 2010;Iyer, Iken & Leon, 2016;Arulazhagan et al, 2017a). In particular, several studies have focused on the use of Stenotrophomonas maltophilia for the remediation of Polycyclic Aromatic Hydrocarbons (PAHs) or of crude oil contaminated sites (Boonchan, Britz & Stanley, 1998;Juhasz, Stanley & Britz, 2000;Arulazhagan et al, 2017b) PAHs are the compounds formed from two or more fused aromatic rings.…”

Section: Introductionmentioning

confidence: 99%

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Supplemental Information 5: This Table Contains the Growth of Pemsol and Other Hydrocarbon Degrading Bacteria Tested for Their

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Background: Stenotrophomonas are ubiquitous gram-negative bacteria which survive in a wide range of environments. They can use many substances for their growth and are known to be intrinsically resistant to many antimicrobial agents. They have been tested for biotechnological applications, bioremediation and antimicrobial agents because of their recalcitrant nature to many toxic compounds. Method. Stenotrophomonas sp. Pemsol was isolated from a crude oil contaminated soil. The capability of this isolate to tolerate and degrade polycyclic aromatic hydrocarbons (PAHs) (anthracene, anthraquinone, biphenyl, naphthalene, phenanthrene, phenanthridine and xylene) was evaluated on Bush Nell Hass medium containing PAHs as the unique carbon sources. The metabolites formed after 30day degradation of naphthalene by Pemsol were analyzed using Fourier Transform Infrared Spectroscopic (FTIR), Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). Results. Complete degradation of naphthalene at a concentration of 1 mg/mL was obtained and a newly formed catechol peak obtained from the UPLC-MS and GC-MS confirmed the degradation. The strain Pemsol lacked the ability to produce biosurfactant so that it cannot bio-emulsify PAHs. The whole genome analysis of Stenotrophomonas sp. Pemsol revealed a wealth of genes for hydrocarbon utilization and interaction with the environment and the presence

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Enzymatic Bioremediation: Current Status, Challenges of Obtaining Process, and Applications

Okino‐Delgado

Zanutto-Elgui

Prado

et al. 2019

Microorganisms for Sustainability

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Characterization and comparison of putativeStenotrophomonas maltophiliamethyl parathion hydrolases

Cited by 8 publications

References 35 publications

The Polycyclic Aromatic Hydrocarbon (PAH) degradation activities and genome analysis of a novel strainStenotrophomonas sp. Pemsol isolated from Mexico

The Polycyclic Aromatic Hydrocarbon (PAH) degradation activities and genome analysis of a novel strainStenotrophomonas sp. Pemsol isolated from Mexico

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Enzymatic Bioremediation: Current Status, Challenges of Obtaining Process, and Applications

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