Characterization and Comparison of Intestinal Bacterial Microbiomes of Euschistus heros and Piezodorus guildinii Collected in Brazil and the United States

Abstract

Background: Herbivorous insects are one of the main biological threats to crops. One such group of insects, stink bugs, do not eat large amounts of tissue when feeding on soybean, but are damaging to the quality of the seed yield as they feed on green developing seeds leading to poorly marketable harvests. In addition to causing physical damage during sucking-feeding activities, the insects can also transmit microbial pathogens, leading to even greater yield loss. Conducting surveys of the insect intestinal microbiome can help identify possible pathogens, as well as detail what healthy stink bug digestive systems have in common.Methods: We used the conserved V4 region of the 16S rRNA gene to characterize the bacterial microbiome of the red-banded stink bug Piezodorus guildinii collected in Brazil and the United States, as well as the neotropical brown stink bug Euschistus heros collected in Brazil.Results: After quality filtering of the data, 192 samples were kept for analyses: 117 samples from P. guildinii covering three sites in Brazil and four sites in the United States, and 75 samples for E. heros covering 10 sites in Brazil. The most interesting observations were that the diversity and abundance of some bacterial families were different in the different ecoregions of Brazil and the United States.Conclusion: Some families, such as Acetobacteraceae, Bacillaceae, Moraxellaceae, Enterobacteriaceae, and Rhodocyclaceae, may be related to the better adaptation in some localities in providing nutrients, break down cellulose, detoxify phytochemicals, and degrade organic compounds, which makes it difficult to control these species.

Tardigrade Community Microbiomes in North American Orchards Include Putative Endosymbionts and Plant Pathogens

“…OTU 2 showed significant temporal variation, decreasing in relative abundance from 10.0 to 0.062% from 2019 to 2020 ( Supplementary Tables S8 , S9 ). Enterobacteriaceae is also highly represented in the gut microbiota of insects ( Hernández-García et al, 2017 ; Moro et al, 2021 ) and nematodes ( Zimmermann et al, 2020 ; Zhou et al, 2022 ). This suggests that Enterobacteriaceae may be residents of the tardigrade digestive tract.…”

Analysis of Intestinal Microbial Diversity of Four Species of Grasshoppers and Determination of Cellulose Digestibility

“…Yuan et al confirmed that the gut bacterial structure of G. molestacan be influenced by the host plant [ 32 ]. Moro et al showed that the diversity of gut microbes of the same species in regions was different [ 33 ]. Jesús M. et al confirmed that different time scales strongly influence the diversity, composition and metabolic capabilities of Brithys crini gut microbial communities [ 34 ].…”

Tardigrade community microbiomes in North American orchards include putative endosymbionts and plant pathogens

“…OTU 2 showed significant temporal variation, decreasing in relative abundance from 10.0% to 0.062% from 2019 to 2020 (Table S8, Table S9). Enterobacteriaceae is also highly represented in the gut microbiota of insects (Moro et al 2021;Hernández-García et al 2017) and nematodes (Zhou et al 2022;Zimmermann et al 2020). This suggests that Enterobacteriaceae may be residents of the tardigrade digestive tract.…”