Characterization and cloning of androgen-repressed mRNAs from rat ventral prostate

Léger, J; Montpetit, Michael L.; Tenniswood, Martin

doi:10.1016/s0006-291x(87)80106-7

Cited by 246 publications

(109 citation statements)

References 21 publications

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“…Although the role of protein synthesis in apoptosis is unclear, the protein synthesis inhibitor cycloheximide has been reported to induce apoptosis in some instances (41). It is therefore possible that the inhibition ofprotein synthesis produced by chromate could be involved in the initiation of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Induction of internucleosomal DNA fragmentation by carcinogenic chromate: relationship to DNA damage, genotoxicity, and inhibition of macromolecular synthesis.

Manning

Blankenship

Wise

et al. 1994

Environ Health Perspect

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Hexavalent chromium (Cr) compounds are respiratory carcinogens in humans and animals. Treatment of Chinese hamster ovary cells with 150 and 300 pM sodium chromate (Na2CrO4) for 2 hr decreased colony-forming efficiency by 46 and 92%, respectively. These treatments induced dosedependent internucleosomal fragmentation of cellular DNA beyond 24 hr after chromate treatment. This fragmentation pattern is characteristic of apoptosis as a mechanism of cell death. These treatments also induced an immediate inhibition of macromolecular synthesis and delayed progression of cells through S-phase of the cell cycle. Cell growth (as evidenced by DNA synthesis) was inhibited for at least 4 days and transcription remained suppressed for at least 32 hr. Many of the cells that did progress to metaphase exhibited chromosome damage. Chromate caused the dose-dependent formation of DNA single-strand breaks and DNA-protein cross-links, but these were repaired 8 and 24 hr after removal of the treatment, respectively. In contrast, Cr-DNA adducts (up to 1/100 base-pairs) were extremely resistant to repair and were still detectable even 5 days after treatment. Compared with other regions of the genome, DNA-protein cross-links and Cr adducts were preferentially associated with the nuclear matrix DNA of treated cells, which was 4.5-fold enriched in actively transcribed genes. Chromium adducts, formed on DNA in vitro at a similar level to that detected in nuclear matrix DNA, arrested the progression of a DNA polymerase in a sequence-specific manner, possibly through the formation of DNA-DNA cross-links. Total RNA and mRNA synthesis and induction of expression of the inducible GRP78 gene were suppressed in a concentration-and time-dependent manner by chromate. The effects of chromate on GRP78 induction correlated most closely with the presence of DNA-protein cross-links but suppression of total RNA and mRNA synthesis correlated with the presence of DNA-Cr adducts in cells. These results suggest that the persistent Cr-DNA adducts may be responsible for the protracted cell cycle delay and transcriptional inhibition caused by chromate. Escape from apoptosis may be one of the steps involved in chromate-induced carcinogenesis. -Environ Health Perspect 102(Suppl 3): 159-167 (1994).

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Section: Discussionmentioning

confidence: 99%

Induction of internucleosomal DNA fragmentation by carcinogenic chromate: relationship to DNA damage, genotoxicity, and inhibition of macromolecular synthesis.

Manning

Blankenship

Wise

et al. 1994

Environ Health Perspect

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“…[6][7][8][9][10] It is a multifunctional protein, which has been implicated in diverse processes as cell aggregation, 6 inhibition of complement-mediated cytotoxicity, 8 and lipid transport. 11 Clusterin has been shown to play an antiapoptotic role in prostate cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Clusterin as a possible predictor for biochemical recurrence of prostate cancer following radical prostatectomy with intermediate Gleason scores: a preliminary report

Pins

Fiadjoe

Korley

et al. 2004

Prostate Cancer Prostatic Dis

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“…Clusterin is a ubiquitous and highly conserved secreted glycoprotein thought to be involved in a variety of biological processes, including lipid transport (1), sperm maturation (2), regulation of the complement cascade (3), apoptosis (4), membrane recycling (5), and possibly others (for review, see Ref. 6).…”

mentioning

confidence: 99%

Multiple Involvement of Clusterin in Chicken Ovarian Follicle Development

Mahon¹,

Lindstedt²,

Hermann

et al. 1999

Journal of Biological Chemistry

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The interaction of the female germ cell with somatic cells during the development of the ovarian follicle in the chicken provides a prime system to study gene expression. Here, we have uncovered the involvement of clusterin, the function(s) of which is still poorly understood, in this complex process. As revealed by molecular cloning, chicken clusterin is a 428-residue protein that migrates at 70 kDa on SDS-polyacrylamide gel electrophoresis and possesses most of the structural features of its mammalian successors. However, in contrast to mammalian clusterin, the chicken protein appears not to be cleaved intracellularly into a disulfide-linked heterodimer; possibly as a consequence thereof, it is not secreted constitutively and is absent from the circulation, where most of clusterin is found in mammals. In the ovary, clusterin is a major product of the somatic granulosa cells, in a pattern correlating with the developmental phases of individual follicles. In that, transcript levels are high not only at onset of vitellogenesis, but also in atretic follicles and in the postovulatory follicle sac, i.e. in situations characterized by apoptotic events. Yolk of growing oocytes contains a 43-kDa truncated form of clusterin that does not appear to be synthesized within the oocyte. Rather, we here show for the first time that 70-kDa clusterin interacts not only with megalin, but also with two chicken oocyte-specific members of the low density lipoprotein receptor (LDLR) gene family. These receptors, termed LDLR-related protein with eight ligand binding repeats (LR8) and LDLR-related protein (380 kDa), likely internalize granulosa cell-derived 70-kDa clusterin, which may subsequently be processed to the 43-kDa product. Thus, chicken clusterin could serve as a marker for follicular atresia and resorption, and, based on its ability to bind several other proteins, it may serve as carrier for the receptor-mediated endocytosis into oocytes of components important for embryonic development, two hitherto unknown functions of this intriguing protein.

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Characterization and cloning of androgen-repressed mRNAs from rat ventral prostate

Cited by 246 publications

References 21 publications

Induction of internucleosomal DNA fragmentation by carcinogenic chromate: relationship to DNA damage, genotoxicity, and inhibition of macromolecular synthesis.

Induction of internucleosomal DNA fragmentation by carcinogenic chromate: relationship to DNA damage, genotoxicity, and inhibition of macromolecular synthesis.

Clusterin as a possible predictor for biochemical recurrence of prostate cancer following radical prostatectomy with intermediate Gleason scores: a preliminary report

Multiple Involvement of Clusterin in Chicken Ovarian Follicle Development

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