Characterization and chromosomal location of Pm40 in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium

Luo, Peigao; Luo, Hongdi; Chang, Zhijian; Zhang, H. Y.; Zhang, M.; Ren, Zhenglong

doi:10.1007/s00122-009-0962-0

Cited by 124 publications

(86 citation statements)

References 22 publications

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“…is a hexaploid species (2n=6x=42) with genomes E 1 E 2 St (Wang et al 1994) or JJ S St (Chen et al 1998). The species has been hybridized extensively with wheat and proved to be a valuable source of genes for disease resistance (Larkin et al 1995;Chen et al 2003;Yang et al 2006;Luo et al 2009) and tolerance to salt stress (Dvořák 1985). The correct identification of Th.…”

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confidence: 99%

A new long terminal repeat (LTR) sequence allows to identify J genome from JS and St genomes of Thinopyrum intermedium

Tang

Yang

et al. 2010

J Appl Genetics

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A repetitive sequence of 491 bp, named pMD232-500, was isolated from S. cereale cv. Kustro using wheat SSR marker Xgwm232. GenBank BLAST search revealed that the sequence of pMD232-500 was highly similar to a part of retrotransposon Nusif-1. Fluorescence in situ hybridization (FISH) analysis using pMD232-500 as probe indicated that only 14 Thinopyrum intermedium chromosomes and all the chromosomes of S. cereale cv. Kustro bear FISH signals, however, no FISH signals were observed on Dasypyrum villosum chromosomes. In addition, the FISH signals were distributed on whole arms except their terminal regions. Further genomic in situ hybridization (GISH) analysis using genomic DNA from Pseudoroegneria spicata indicated that the 14 Th. intermedium chromosomes bearing FISH signals should belong to J genome. Thereafter, the repetitive elements pMD232-500 showed the unambiguous features of genomic constitution of Th. intermedium. In addition, the results in the present study have indicated the similarity of genomes from Th. intermedium and S. cereale.

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confidence: 99%

A new long terminal repeat (LTR) sequence allows to identify J genome from JS and St genomes of Thinopyrum intermedium

Tang

Yang

et al. 2010

J Appl Genetics

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“…The results showed that SN100109 was immune to E01, E18, E23-2, E30-1, and E32; and highly resistant to E09, E17, E21, E23-1, E26, E31, E49, and E50; and resistant to E02, E13, and E16. Compared with Pm40 (Luo et al 2009) and Pm43 (He et al 2009), which also derived from Th. intermedium, SN100109 and GRY19 (Pm40) had different resistance reactions to Bgt races E05, E06, E07, E16, and E21.…”

Section: Characteristics Of Sn100109 Resistance To Powdery Mildewmentioning

confidence: 95%

“…The results infer that the resistance gene in SN100109 lies on the additional 2J chromosomes. Gene Pm40 (Luo et al 2009) and Pm43 (He et al 2009) also derived from Th. intermedium.…”

Section: Resistance To Powdery Mildew In Sn100109mentioning

confidence: 99%

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Cytogenetic and molecular identification of a new wheat-Thinopyrum intermediumaddition line with resistance to powdery mildew

Qi¹,

Li²,

He³

et al. 2015

Cereal Research Communications

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Thinopyrum intermedium, which has many useful traits, is valuable for wheat breeding. A new wheat-Thinopyrum addition line, SN100109, was developed from the progeny of common wheat cultivar Yannong 15 and Th. intermedium. It was resistant to most races of Blumeria graminis f. sp tritici (Bgt), which caused powdery mildew in wheat, and its reactions were different from the reactions of gene Pm40 and Pm43. Genomic in situ hybridization (GISH) and molecular marker analysis were used to identify the genomic composition of SN100109. GISH results showed that SN100109 was a wheat-Th. intermedium disomic addition line containing one pair of J chromosomes, and the resistance gene was located on the alien additional chromosomes of SN100109. And four molecular markers BE425942, BF482714, Xgdm93 and BV679214 which were assigned to homologous group 2, were specific molecular markers of the additional chromosomes. All the results indicated that SN100109 contained one pair of 2J chromosomes. SN100109 can be used as a novel germplasm source for introducing powdery mildew resistance genes to wheat in breeding programs.

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“…The shift in pathogenic virulence structures range from the southwest and the southeast coastal regions of China to almost all the wheat growing areas throughout the country and the damage exceeds any other known wheat disease (Liu and Shao, 1994;Luo et al, 2009;Cao et al, 2013;Zhao et al, 2013;Shen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of disease resistance genes to powdery mildew (Blumeria graminis f. sp. tritici) in wheat (Triticum aestivum) cultivars

Mwale¹,

Tang²,

Chilembwe³

2017

Afr. J. Biotechnol.

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A study was conducted to detect the presence of disease resistance genes to infection of wheat powdery mildew (Blumeria graminis f. sp. tritici) in selected wheat cultivars from China using molecular markers. Genomic DNA of sixty cultivars was extracted and tested for the presence of selected prominent resistance genes to the fungal disease using molecular markers linked to Pm genes. Results showed that 17 cultivars were detected with Pm2 gene, 24 cultivars were detected with Pm4b gene, two cultivars were detected with Pm6 gene while 24 wheat cultivars were detected with Pm8 gene. Multiple genes were also detected in the study. Cultivars Xinxuan2039, Lankao008 and Zhengmai366 were detected with possible multiple Pm2+Pm4b+Pm8 genes while Yumai368 was detected with possible multiple genes Pm2+Pm4b+Pm6. The results of this study provide a significant contribution to breeding for resistance to wheat powdery mildew disease since the identified cultivars detected with Pm genes will contribute to further studies on improving wheat resistance to the disease. Also, the continued resistance of cultivars with designated resistance genes demonstrates that the responsible Pm genes are still effective in overcoming powdery mildew infections.

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Characterization and chromosomal location of Pm40 in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium

Cited by 124 publications

References 22 publications

A new long terminal repeat (LTR) sequence allows to identify J genome from JS and St genomes of Thinopyrum intermedium

A new long terminal repeat (LTR) sequence allows to identify J genome from JS and St genomes of Thinopyrum intermedium

Cytogenetic and molecular identification of a new wheat-Thinopyrum intermediumaddition line with resistance to powdery mildew

Molecular detection of disease resistance genes to powdery mildew (Blumeria graminis f. sp. tritici) in wheat (Triticum aestivum) cultivars

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