Characterization and chemosensitivity of a human epithelioid sarcoma cell line (SARCCR 2)

Abstract

A new cell line was derived from the epithelioid sarcoma of a Caucasian woman who had previously received chemotherapy. The cells grew as an adherent monolayer, with a doubling time of 28 hr and had mainly epithelial morphology, but with areas of mesenchymal-like cytoplasmic extensions. The cells were tumorigenic in nude mice, with a short growth time, and a doubling time of 8 days. The cell line showed over-expression of P-glycoprotein by Western blot analysis, and its sensitivity to doxorubicin and vincristine was low. This sensitivity could be enhanced by reversants of multidrug resistance (MDR), such as cyclosporin or verapamil. This cell line constitutes an excellent model for studying compounds able to reverse MDR.

Growth inhibitory effects of paclitaxel on human epithelioid sarcoma in vitro

“…[2][3][4][5]34,35 In contrast, our study demonstrates for the first time that paclitaxel inhibits the proliferation of human epithelioid sarcoma in vitro. Because plasma peak concentrations of 1 to 10 M paclitaxel previously had been achieved in clinical trials, 6 clinically relevant concentrations of paclitaxel yielded antiproliferative effects in our study.…”

“…From a clinical point of view, epithelioid sarcoma is characterized by an extremely adverse clinical course showing a high frequency of recurrence and metastasis 2 as well as a pronounced resistance against conventional chemotherapy and radiotherapy. [2][3][4][5] Recently, paclitaxel has been introduced as a novel anticancer agent showing activity against a broad range of human tumors, especially therapy-refractory ovarian and breast carcinoma as well as nonsmall cell lung carcinoma. 6 -11 Although the microtubule is con-sidered to be the main molecular target of paclitaxel, [12][13][14][15] other cellular actions of paclitaxel have been observed as well.…”

Epithelioid Sarcoma: Opportunities for Biology-Driven Targeted Therapy

“…A study of the SARCCR2 cell line showed overexpression of P-glycoprotein, an ATP-binding cassette (ABC) chemotherapy export pump. Using verapamil and cyclosporine A to reverse multidrug resistance, the authors showed increased sensitivity to doxorubicin and vincristine ( 83 ). For the GRU cell line, expression of P-glycoprotein and MRP could also be observed ( 84 ).…”