Characterization and cDNA Cloning of a Defensin-Like Peptide, Harmoniasin, from Harmonia axyridis

Kim, In Woo; Lee, Joon Ha; Park, Ha Yan; Kwon, Young Nam; Yun, Eun Young; Nam, Sung Hee; Kim, Seong Ryul; Ahn, Mi Young; Hwang, Jae Sam

doi:10.4014/jmb.1206.06064

Cited by 5 publications

(11 citation statements)

References 13 publications

(14 reference statements)

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“…In addition, it has been reported that bovine lactoferricin binds to the cell membrane and causes cell membrane disruption followed by entry of the peptide to the cytoplasm of Jurkat T-leukemia cells and damage to mitochondrial membrane (34). Based on the results of our previous study (17), it was postulated that HaA4 acts on anticancer activity similar to bovine lactoferricin, although the exact mechanism of HaA4 has to be elucidated.…”

Section: Haa4 Induces Apoptosis In the Leukemia Cells Via A Caspasedementioning

confidence: 99%

“…Harmoniasin is a defensin-like peptide consisting of 50 amino acid residues with three intra-disulfide bonds. Because of the large molecular weight and disulfide bonds, we designed a variety of analogues based on the harmoniasin sequence in a previous study (17). The resulting homodimer peptide, named HaA4, was synthesized and provided by Anygen Co., Ltd. (Gwangju, Korea).…”

Section: Methodsmentioning

confidence: 99%

“…Active region of harmoniasin was defined and selected to be modified as a homodimeric peptide. HaA4 displayed more potent antibacterial activity than that of the native peptide (17). HaA4 might also retain cytotoxic effect on cancer cells similarly to some other AMPs.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we showed that synthetic HaA4 exerts antibacterial effect without hemolytic activities (17). We attempted to determine the effect of the synthetic peptide HaA4 on cell growth and survival of human leukemia cells (Jurkat and U937) in this study.…”

Section: Haa4 Markedly Decreases Cell Viability Of Leukemia Cell Linesmentioning

confidence: 99%

“…Base on the results, we surmised that lytic activity of HaA4 is influenced by the presence of serum. Although HaA4 had no hemolytic activity in our previous report (17), cell selectivity of HaA4 including serum stability needs to be examined further. Finally, when compared to the results from the MTS assay, the reduction in cell viability was 30-40% higher than expected from cytotoxicity.…”

Section: Effect Of Haa4 On the Integrity Of Cancer Cell Membranementioning

confidence: 99%

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Anticancer activity of a synthetic peptide derived from harmoniasin, an antibacterial peptide from the ladybug Harmonia axyridis

Kim

Lee

Kwon

et al. 2013

International Journal of Oncology

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Abstract.Harmoniasin is a defensin-like antimicrobial peptide identified from the ladybug Harmonia axyridis. Among the synthetic homodimer peptide analogues derived from harmoniasin, HaA4 has been found to have antibacterial activity without hemolytic activity. In this study, we investigated whether HaA4 has anticancer activity against human leukemia cell lines such as U937 and Jurkat cells. HaA4 manifested cytotoxicity and decreased the cell viability of U937 and Jurkat cells in MTS assay and LDH release assay. We found that HaA4 induced apoptotic and necrotic cell death of the leukemia cells using flow cytometric analysis, acridine orange/ethidium bromide staining and nucleosomal fragmentation of genomic DNA. Activation of caspase-7 and -9 and fragmentation of poly (ADP-ribose) polymerase was detected in the HaA4-treated leukemia cells, suggesting induction of a caspase-dependent apoptosis pathway by HaA4. Caspase-dependent apoptosis was further confirmed by reversal of the HaA4-induced viability reduction by treatment of Z-VAD-FMK, a pan-caspase inhibitor. In conclusion, HaA4 caused necrosis and caspase-dependent apoptosis in both U937 and Jurkat leukemia cells, which suggests potential utility of HaA4 as a cancer therapeutic agent.

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Section: Haa4 Induces Apoptosis In the Leukemia Cells Via A Caspasedementioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Haa4 Markedly Decreases Cell Viability Of Leukemia Cell Linesmentioning

confidence: 99%

Section: Effect Of Haa4 On the Integrity Of Cancer Cell Membranementioning

confidence: 99%

See 3 more Smart Citations

Anticancer activity of a synthetic peptide derived from harmoniasin, an antibacterial peptide from the ladybug Harmonia axyridis

Kim

Lee

Kwon

et al. 2013

International Journal of Oncology

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Building HMM and molecular docking analysis for the sensitive detection of anti-viral pneumonia antimicrobial peptides (AMPs)

Bakare

Keyster

Pretorius

2021

Sci Rep

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Pneumonia is the main reason for mortality among children under five years, causing 1.6 million deaths every year; late research has exhibited that mortality is increasing in the elderly. A few biomarkers used for its diagnosis need specificity and precision, as they are related to different infections, for example, pulmonary tuberculosis and Human Immunodeficiency Virus. There is a quest for new biomarkers worldwide to diagnose the disease to defeat these previously mentioned constraints. Antimicrobial peptides (AMPs) are promising indicative specialists against infection. This research work used AMPs as biomarkers to detect viral pneumonia pathogens, for example, Respiratory syncytial virus, Influenza A and B viruses utilizing in silico technologies, such as Hidden Markov Model (HMMER). HMMER was used to distinguish putative anti-viral pneumonia AMPs against the recognized receptor proteins of Respiratory syncytial virus, Influenza A, and B viruses. The physicochemical parameters of these putative AMPs were analyzed, and their 3-D structures were determined utilizing I-TASSER. Molecular docking interaction of these AMPs against the recognized viral pneumonia proteins was carried out using the PATCHDOCK and HDock servers. The results demonstrated 27 anti-viral AMPs ranked based on their E values with significant physicochemical parameters in similarity with known experimentally approved AMPs. The AMPs additionally had a high anticipated binding potential to the pneumonia receptors of these microorganisms sensitively. The tendency of the putative anti-viral AMPs to bind pneumonia proteins showed that they would be promising applicant biomarkers to identify these viral microorganisms in the point-of-care (POC) pneumonia diagnostics. The high precision observed for the AMPs legitimizes HMM’s utilization in the disease diagnostics’ discovery process.

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Identification of biomarkers for the accurate and sensitive diagnosis of three bacterial pneumonia pathogens using in silico approaches

Bakare

Keyster

Pretorius

2020

BMC Mol and Cell Biol

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Background Pneumonia ranks as one of the main infectious sources of mortality among kids under 5 years of age, killing 2500 a day; late research has additionally demonstrated that mortality is higher in the elderly. A few biomarkers, which up to this point have been distinguished for its determination lack specificity, as these biomarkers fail to build up a differentiation between pneumonia and other related diseases, for example, pulmonary tuberculosis and Human Immunodeficiency Infection (HIV). There is an inclusive global consensus of an improved comprehension of the utilization of new biomarkers, which are delivered in light of pneumonia infection for precision identification to defeat these previously mentioned constraints. Antimicrobial peptides (AMPs) have been demonstrated to be promising remedial specialists against numerous illnesses. This research work sought to identify AMPs as biomarkers for three bacterial pneumonia pathogens such as Streptococcus pneumoniae, Klebsiella pneumoniae, Acinetobacter baumannii using in silico technology. Hidden Markov Models (HMMER) was used to identify putative anti-bacterial pneumonia AMPs against the identified receptor proteins of Streptococcus pneumoniae, Klebsiella pneumoniae, and Acinetobacter baumannii. The physicochemical parameters of these putative AMPs were computed and their 3-D structures were predicted using I-TASSER. These AMPs were subsequently subjected to docking interaction analysis against the identified bacterial pneumonia pathogen proteins using PATCHDOCK. Results The in silico results showed 18 antibacterial AMPs which were ranked based on their E values with significant physicochemical parameters in conformity with known experimentally validated AMPs. The AMPs also bound the pneumonia receptors of their respective pathogens sensitively at the extracellular regions. Conclusions The propensity of these AMPs to bind pneumonia pathogens proteins justifies that they would be potential applicant biomarkers for the recognizable detection of these bacterial pathogens in a point-of-care POC pneumonia diagnostics. The high sensitivity, accuracy, and specificity of the AMPs likewise justify the utilization of HMMER in the design and discovery of AMPs for disease diagnostics and therapeutics.

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Characterization and cDNA Cloning of a Defensin-Like Peptide, Harmoniasin, from Harmonia axyridis

Cited by 5 publications

References 13 publications

Anticancer activity of a synthetic peptide derived from harmoniasin, an antibacterial peptide from the ladybug Harmonia axyridis

Anticancer activity of a synthetic peptide derived from harmoniasin, an antibacterial peptide from the ladybug Harmonia axyridis

Building HMM and molecular docking analysis for the sensitive detection of anti-viral pneumonia antimicrobial peptides (AMPs)

Identification of biomarkers for the accurate and sensitive diagnosis of three bacterial pneumonia pathogens using in silico approaches

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