Characterization and Catalytic Properties of Free and Silica-Bound Lipase: a Comparative Study

Narwal, Sunil Kumar; Saun, Nitin Kumar; Gupta, Reena

doi:10.5650/jos.ess13231

Cited by 16 publications

(9 citation statements)

References 24 publications

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“…After 6-hour incubation, the immobilized lipase still maintained the high activity at 95%, whereas the native lipase activity decreased after 2 hours to 60% and dramatically dropped to 40% after 6-hour incubation. This phenomenon was similar to previous reports [18, 37, 47] in which the immobilized lipase is obviously stable in comparison to soluble native form in terms of long incubation period at high temperature. Taken into consideration, the thermal stability of LPA complex will be useful when it would have been applied to increase hydrolysis yield by extending of incubation time and to treat recalcitrant substrates, for example, waste cooking oil, in near future.…”

Section: Resultssupporting

confidence: 92%

Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production

Dumri

Anh

2014

Enzyme Research

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Biodiesel production technology is competitive in terms of low cost and alternative source of energy which should be not only sustainable but also environmentally friendly. Designing of the lipase immobilization for biodiesel production has a remarkable impact and is still challenging. In this work, biodiesel production from soybean oil was enhanced and facilitated by using a novel biocatalyst consisting of commercial lipase (EC 3.1.1.3), silver nanoparticles, and polydopamine. Silver nanoparticles (AgNPs) were synthesized with a size range of 10–20 nm. Polydopamine (PD) was delivered by the self-polymerization of dopamine in 10 mM Tris-HCl pH 8.5 and simultaneously coated the AgNPs to form a PD/AgNPs complex. Lipase was immobilized on the PD/AgNPs complex surface via covalent bonds to form a tailor-made biocatalyst consisting of immobilized lipase/PD/AgNPs complex (LPA). The formation and morphology of each composition were characterized by UV-Vis spectroscopy and scanning electron microscope (SEM). Significantly, gas chromatography analysis showed a remarkable biodiesel production yield of 95% by using the LPA complex at 40°C for 6-hours reaction time, whereas the yield was 86% when using free lyophilized lipase. The LPA complex was apparently reusable after 7 batches and the latter conversion rate of soybean oil was decreased by only 27%.

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Section: Resultssupporting

confidence: 92%

Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production

Dumri

Anh

2014

Enzyme Research

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“…These protect the tertiary and quaternary structure of the enzyme from conformational changes. Additionally, binding of the enzyme to the carrier stabilizes the whole enzyme particle and preserves it against denaturation under harsh reaction conditions [50]. The results presented by Zhu et al [51] also show an increase in the pH resistance of the immobilized lipase.…”

Section: Immobilized Lipase Stabilitymentioning

confidence: 96%

Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization

et al. 2017

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The main purpose of the study was to achieve effective immobilization of lipase B from Candida antarctica (CALB) onto 3D spongin-based scaffolds from Hippospongia communis marine demosponge for rapeseed oil transesterification. Successful immobilization onto the marine sponge skeleton was confirmed for the first time. Lipase B-containing biocatalytic system exhibited the highest catalytic activity retention (89%) after 60 min of immobilization at pH 7 and temperature of 4 • C. Immobilization was found to improve the thermal and chemical stability compared to free lipase, and retain over 80% of its initial catalytic activity over a wide range of temperature (30-60 • C) and pH (6-9). Additionally, immobilized lipase has good storage stability and retains over 70% of its initial activity even after catalyzing of 25 reaction cycles. The obtained product was used in a transesterification reaction of rapeseed oil with methanol and proved to be an efficient biocatalyst for biofuel production. The highest conversion value and fatty acids methyl esters (FAME) concentration were observed after a process conducted at 40 • C and pH 10. The possible mechanism of interaction between the enzyme and the spongin-based support is proposed and discussed.

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“…The biocatalytic systems obtained demonstrate high catalytic activity retention and good thermal and pH resistance. For example, lipases immobilized on a silica gel matrix and on mesoporous silica retained respectively 91% and 96% of the activity of the free enzyme [22,23].…”

Section: Silica and Inorganic Oxidesmentioning

confidence: 99%

A General Overview of Support Materials for Enzyme Immobilization: Characteristics, Properties, Practical Utility

et al. 2018

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In recent years, enzyme immobilization has been presented as a powerful tool for the improvement of enzyme properties such as stability and reusability. However, the type of support material used plays a crucial role in the immobilization process due to the strong effect of these materials on the properties of the produced catalytic system. A large variety of inorganic and organic as well as hybrid and composite materials may be used as stable and efficient supports for biocatalysts. This review provides a general overview of the characteristics and properties of the materials applied for enzyme immobilization. For the purposes of this literature study, support materials are divided into two main groups, called Classic and New materials. The review will be useful in selection of appropriate support materials with tailored properties for the production of highly effective biocatalytic systems for use in various processes.

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Characterization and Catalytic Properties of Free and Silica-Bound Lipase: a Comparative Study

Cited by 16 publications

References 24 publications

Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production

Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production

Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization

A General Overview of Support Materials for Enzyme Immobilization: Characteristics, Properties, Practical Utility

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