Characterization and Carbon Metabolism in Fungi Pathogenic to Triatoma infestans, a Chagas Disease Vector

Juárez, M. Patricia; Crespo, Rosana; Fernández, G. Calderón; Lecuona, Roberto E.; Cafferata, Lázaro F. R.

doi:10.1006/jipa.2000.4964

Cited by 24 publications

(12 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Table I. Fatty acid composition ± SEM (n = 6) expressed as total fatty acid methyl esters (% of total fatty acid) of polycentric fungi (upper part) and monocentric fungi (lower part) † P o l y c e n t r i c f u n g i In the enthomopathogenic fungus Beauveria bassiana behenic acid was the major component and saturated VLCFAs with 21, 22, 24, 26 and 28 carbons were identified in small amounts (Juarez et al 2000). In our anaerobic fungal strains unsaturated fatty acids, including cis-11-eicosenoic, cis-11,14-eicosadienoic, erucic, 13,16-docosadienoic and nervonic acids represent very small amounts (<0.75 % of total FA), but their incidence in aerobic fungi has been reported here for the first time.…”

Section: Resultsmentioning

confidence: 99%

Analysis of fatty acid composition of anaerobic rumen fungi

et al. 2008

View full text Add to dashboard Cite

The fatty acid (FA) composition of fresh mycelia of anaerobic rumen fungi was determined. The fatty acids methyl esters (FAME) of six strains belonging to four genera (Neocallimastix, Caecomyces, Orpinomyces, Anaeromyces) and one unknown strain were analyzed by gas chromatography. All studied fungi possess the same FAs but differences were found in their relative concentrations. The FA profile of anaerobic fungi comprises carbon chains of length ranging from 12 to 24; the most common fatty acids were stearic (C(18:0)), arachidic (C(20:0)), heneicosanoic (C(21:0)), behenic (C(22:0)), tricosanoic (C(23:0)) and lignoceric (C(24:0)) with relative amount representing >4% of total FA. Significant differences were determined for heptadecanoic, oleic, behenic and tricosanoic acids. Rumen anaerobic fungi can contain very long chain fatty acids; we found unsaturated fatty acids including cis-11-eicosenoic (C(20:1)), cis-11,14-eicosadienoic (C(20:2)), erucic (C(22:1n9)), cis-13,16-docosadienoic (C(22:2)) and nervonic (C(24:1)) acids in very small amounts but their presence seems to be unique for anaerobic fungi.

show abstract

Section: Resultsmentioning

confidence: 99%

Analysis of fatty acid composition of anaerobic rumen fungi

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Several experiments have documented the efficacy of entomopathogenic fungi, such as Beauveria bassiana and Metarhizium anisopliae, in the control of Chagas disease vectors under laboratory [24][25][26][27][28] and fi eld conditions [29][30][31][32] . The fi rst barrier to the penetration of contact insecticides is the insect cuticle, and an increase in the thickness of the Triatoma infestans cuticle was found to be associated with the resistance of this insect to pyrethroids.…”

Section: Methodsmentioning

confidence: 99%

Potential for entomopathogenic fungi to control Triatoma dimidiata (Hemiptera: Reduviidae), a vector of Chagas disease in Mexico

Vázquez-Martínez

Cirerol-Cruz

Torres-Estrada

et al. 2014

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

“…Fungi were maintained on complete medium agar (CMA) plates containing 0.4 g KH 2 PO 4 , 1.4 g Na 2 HPO 4 , 0.6 g MgSO 4 .7H 2 O, 1.0 g KCl, 0.7 g NH 4 NO 3 .7H 2 O, 10 g glucose, 5 g yeast extract, and 15 g agar in 1000 ml of distilled water. Glucose-grown (GG) fungi were obtained by culturing on CMA at 26°C for 14 d. Assays were also performed on CMA lacking glucose and yeast extract (deficient medium agar, DMA), and supplemented with the synthetic hydrocarbon n -octacosane ( n -C28) (Sigma, St. Louis, USA), as previously described [16]. Briefly, the hydrocarbon (2.5 ml of a 10% hexane solution, w/v) was layered onto the surface media and the solvent was evaporated.…”

Section: Methodsmentioning

confidence: 99%

Control of Pyrethroid-Resistant Chagas Disease Vectors with Entomopathogenic Fungi

et al. 2009

View full text Add to dashboard Cite

Background Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region—which extends through northern Argentina, Bolivia, and Paraguay—are both recurrent reinfestations after insecticide spraying and emerging pyrethroid-resistance over the past ten years. Among alternative control tools, the pathogenicity of entomopathogenic fungi against triatomines is already known; furthermore, these fungi have the ability to fully degrade hydrocarbons from T. infestans cuticle and to utilize them as fuel and for incorporation into cellular components.Methodology and FindingsHere we provide evidence of resistance-related cuticle differences; capillary gas chromatography coupled to mass spectrometry analyses revealed that pyrethroid-resistant bugs have significantly larger amounts of surface hydrocarbons, peaking 56.2±6.4% higher than susceptible specimens. Also, a thicker cuticle was detected by scanning electron microscopy (32.1±5.9 µm and 17.8±5.4 µm for pyrethroid-resistant and pyrethroid-susceptible, respectively). In laboratory bioassays, we showed that the virulence of the entomopathogenic fungi Beauveria bassiana against T. infestans was significantly enhanced after fungal adaptation to grow on a medium containing insect-like hydrocarbons as the carbon source, regardless of bug susceptibility to pyrethroids. We designed an attraction-infection trap based on manipulating T. infestans behavior in order to facilitate close contact with B. bassiana. Field assays performed in rural village houses infested with pyrethroid-resistant insects showed 52.4% bug mortality. Using available mathematical models, we predicted that further fungal applications could eventually halt infection transmission.ConclusionsThis low cost, low tech, ecologically friendly methodology could help in controlling the spread of pyrethroid-resistant bugs.

show abstract

Characterization and Carbon Metabolism in Fungi Pathogenic to Triatoma infestans, a Chagas Disease Vector

Cited by 24 publications

References 22 publications

Analysis of fatty acid composition of anaerobic rumen fungi

Analysis of fatty acid composition of anaerobic rumen fungi

Potential for entomopathogenic fungi to control Triatoma dimidiata (Hemiptera: Reduviidae), a vector of Chagas disease in Mexico

Control of Pyrethroid-Resistant Chagas Disease Vectors with Entomopathogenic Fungi

Contact Info

Product

Resources

About