Characterization and Bioactivity Evaluation of (Polyetheretherketone/Polyglycolicacid)-Hydroyapatite Scaffolds for Tissue Regeneration

Shuai, Cijun; Shuai, Chenying; Wu, Ping; Yuan, Fulai; Feng, Pei; Guo, Wang; Fan, Xiaohan; Su, Ting; Peng, Shuping; Gao, Chengde

doi:10.3390/ma9110934

Cited by 34 publications

(28 citation statements)

References 33 publications

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“…Thus, the mineral particles of hydroxyapatite formed during SBF soaking significantly strengthened the elastic porous structures. The addition of hydroxyapatite (synthetic or natural) to the polymeric tissue scaffolds most likely increases their compressive strength [ 47 , 48 ]. However, the process of isolation HAp from the SBF solution involves simultaneous hydrolytic degradation, thus a mass loss of the sample.…”

Section: Resultsmentioning

confidence: 99%

Processing of Polyester-Urethane Filament and Characterization of FFF 3D Printed Elastic Porous Structures with Potential in Cancellous Bone Tissue Engineering

et al. 2020

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This paper addresses the potential of self-made polyester-urethane filament as a candidate for Fused Filament Fabrication (FFF)-based 3D printing (3DP) in medical applications. Since the industry does not provide many ready-made solutions of medical-grade polyurethane filaments, we undertook research aimed at presenting the process of thermoplastic polyurethane (TPU) filament formation, detailed characteristics, and 3DP of specially designed elastic porous structures as candidates in cancellous tissue engineering. Additionally, we examined whether 3D printing affects the structure and thermal stability of the filament. According to the obtained results, the processing parameters leading to the formation of high-quality TPU filament (TPU_F) were captured. The results showed that TPU_F remains stable under the FFF 3DP conditions. The series of in vitro studies involving long- and short-term degradation (0.1 M phosphate-buffered saline (PBS); 5 M sodium hydroxide (NaOH)), cytotoxicity (ISO 10993:5) and bioactivity (simulated body fluid (SBF) incubation), showed that TPU printouts possessing degradability of long-term degradable tissue constructs, are biocompatible and susceptible to mineralization in terms of hydroxyapatite (HAp) formation during SBF exposure. The formation of HAp on the surface of the specially designed porous tissue structures (PTS) was confirmed by scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDS) studies. The compression test of PTS showed that the samples were strengthened due to SBF exposure and deposited HAp on their surface. Moreover, the determined values of the tensile strength (~30 MPa), Young’s modulus (~0.2 GPa), and compression strength (~1.1 MPa) allowed pre-consideration of TPU_F for FFF 3DP of cancellous bone tissue structures.

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Section: Resultsmentioning

confidence: 99%

Processing of Polyester-Urethane Filament and Characterization of FFF 3D Printed Elastic Porous Structures with Potential in Cancellous Bone Tissue Engineering

et al. 2020

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“…Numerous applications for the medical field have been developed using AM technology, such as anatomical mockups for surgery training, planning or simulation [ 1 , 2 ], personalized surgical guides [ 3 , 4 ], tissue engineering scaffolds [ 5 , 6 ], customized implants [ 7 , 8 ], etc. Not all these applications are based on FFF, which usually uses thermoplastics, such as ABS (acrylonitrile butadiene styrene), PC (polycarbonate), PLA (polylactic acid), PETG (polyethylene terephthalate glycol), Nylon PA (polyamide), PEEK (poly-ether-ether-ketone) [ 9 ], or their composites.…”

Section: Introductionmentioning

confidence: 99%

Magnesium Filled Polylactic Acid (PLA) Material for Filament Based 3D Printing

et al. 2019

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The main objective of this research is to prove the viability of obtaining magnesium (Mg) filled polylactic acid (PLA) biocomposites as filament feedstock for material extrusion-based additive manufacturing (AM). These materials can be used for medical applications, thus benefiting of all the advantages offered by AM technology in terms of design freedom and product customization. Filaments were produced from two PLA + magnesium + vitamin E (α-tocopherol) compositions and then used for manufacturing test samples and ACL (anterior cruciate ligament) screws on a low-cost 3D printer. Filaments and implant screws were characterized using SEM (scanning electron microscopy), FTIR (fourier transform infrared spectrometry), and DSC (differential scanning calorimetry) analysis. Although the filament manufacturing process could not ensure a uniform distribution of Mg particles within the PLA matrix, a good integration was noticed, probably due to the use of vitamin E as a precursor. The results also show that the composite biomaterials can ensure and maintain implant screws structural integrity during the additive manufacturing process.

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“…Inorganic compounds such as hydroxyapatite among other calcium phosphates are frequently used for the fabrication of bone substitutes for tissue engineering purposes either in combination with polymers [24][25][26][27][28][29] or with collagen to mimic the natural bone composition [30][31][32][33][34][35]. In a recent work of Linh and co-authors, the surface of porous hydroxyapatite scaffolds was modified by collagen treatment and BMP-2 conjugation [36].…”

Section: Discussionmentioning

confidence: 99%

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

et al. 2020

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Extensive efforts were undertaken to develop suitable biomaterials for tissue engineering (TE) applications. To facilitate clinical approval processes and ensure the success of TE applications, bioinspired concepts are currently focused on. Working on bone tissue engineering, we describe in the present study a method for biofunctionalization of collagen/hydroxyapatite composites with BMP-2 mimetic peptides. This approach is expected to be fundamentally transferable to other tissue engineering fields. A modified BMP-2 mimetic peptide containing a negatively charged poly-glutamic acid residue (E7 BMP-2 peptide) was used to bind positively charged hydroxyapatite (HA) particles by electrostatic attraction. Binding efficiency was biochemically detected to be on average 85% compared to 30% of BMP-2 peptide without E7 residue. By quartz crystal microbalance (QCM) analysis, we could demonstrate the time-dependent dissociation of the BMP-2 mimetic peptides and the stable binding of the E7 BMP-2 peptides on HA-coated quartz crystals. As shown by immunofluorescence staining, alkaline phosphatase expression is similar to that detected in jaw periosteal cells (JPCs) stimulated with the whole BMP-2 protein. Mineralization potential of JPCs in the presence of BMP-2 mimetic peptides was also shown to be at least similar or significantly higher when low peptide concentrations were used, as compared to JPCs cultured in the presence of recombinant BMP-2 controls. In the following, collagen/hydroxyapatite composite materials were prepared. By proliferation analysis, we detected a decrease in cell viability with increasing HA ratios. Therefore, we chose a collagen/hydroxyapatite ratio of 1:2, similar to the natural composition of bone. The following inclusion of E7 BMP-2 peptides within the composite material resulted in significantly elevated long-term JPC proliferation under osteogenic conditions. We conclude that our advanced approach for fast and cost-effective scaffold preparation and biofunctionalization is suitable for improved and prolonged JPC proliferation. Further studies should prove the functionality of composite scaffolds in vivo.

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Characterization and Bioactivity Evaluation of (Polyetheretherketone/Polyglycolicacid)-Hydroyapatite Scaffolds for Tissue Regeneration

Cited by 34 publications

References 33 publications

Processing of Polyester-Urethane Filament and Characterization of FFF 3D Printed Elastic Porous Structures with Potential in Cancellous Bone Tissue Engineering

Processing of Polyester-Urethane Filament and Characterization of FFF 3D Printed Elastic Porous Structures with Potential in Cancellous Bone Tissue Engineering

Magnesium Filled Polylactic Acid (PLA) Material for Filament Based 3D Printing

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

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