Characterization and bioactivities of phospholipids from squid viscera and gonads using ultra-performance liquid chromatography-Q-exactive orbitrap/mass spectrometry-based lipidomics and zebrafish models

Abstract

There has been a great interest in the phospholipids (PLs) from marine by-products due to their long chain polyunsaturated fatty acids with unique health and functional properties. Here, the marine...

Comparative bioactivity profile of phospholipids from three marine byproducts based on the zebrafish model

“…In addition, PLs can promote fat metabolism, reduce serum cholesterol, improve blood circulation, and prevent fatty liver and cardiovascular diseases (Hannun & Obeid, 2008; Lee et al, 2010; Liu et al, 2016; Lordan et al, 2020; Xia et al, 2020; Zlomke et al, 2020). Marine PLs have unique advantages in physiological function and biological activity due to their high contents of PUFAs, such as docosahexaenoic acid and eicosapentaenoic acid (Ahmmed et al, 2021; Hassane et al, 2020); they have been shown in detail to exhibit anti‐inflammatory effects and prevent cardiovascular diseases (Li, Zhang, Xie, et al, 2021; Lordan et al, 2020). Marine byproducts such as SH, SG, and CR are rich in PLs (Li et al, 2020; Li, Zhang, Xie, et al, 2021; Takahashi & Inoue, 2012; Zhang et al, 2021) and therefore have a high utilization value.…”

“…In addition, the PL molecular structure and content also may affect their biological activity and nutritional value (Li, Zhang, Xie, et al, 2021; Zhang et al, 2019). According to previous studies, PC, LPC, and PE are the main components of crude PL extracts (Li et al, 2018).…”

“…Marine PLs were extracted from SH, CR, and SG by the previous method with some modification (Li, Zhang, Xie, et al, 2021). First, 100 g of SH, CR, and SG was weighed, homogenized, and then added to 500 ml of 95% ethanol for ultrasonic extraction twice, 1 hour each time.…”

“…At present, the zebrafish has been recommended as a new alternative animal by the European Center for the Validation of Alternative Methods (Eimon & Rubinstein, 2009), becoming the second priority vertebrate model animal after mice. Many human diseases such as cancer, diabetes, cardiovascular and cerebrovascular diseases, and obesity have been successfully modeled on zebrafish (Firus Khan et al, 2020; Katikaneni et al, 2020; Li, Zhang, Xie, et al, 2021; Song & Cone, 2007).…”

Pro-angiogenic New Chloro-Azaphilone Derivatives From the Hadal Trench-Derived Fungus Chaetomium globosum YP-106

“…(Jude et al, 2010;Kurusamy et al, 2017). In recent years, pro-angiogenesis has been highly valued as a new target for the development of cardiovascular drugs, and various therapeutic angiogenesis studies have been carried out in animal models and clinical practice (Li et al, 2021;Zhou et al, 2021). Clinical therapeutic angiogenesis approaches are diverse, such as cytokine therapy, endothelial progenitor cell therapy, stem cell therapy, gene therapy, and mechanical therapy.…”