Characterization and assessment of two biocontrol bacteria against Pseudomonas syringae wilt in Solanum lycopersicum and its genetic responses

Durairaj, Kaliannan; Velmurugan, Palanivel; Park, Junghee; Chang, Woo-Suk; Park, Yool‐Jin; Senthilkumar, Palaninaicker; Choi, Kyung-Min; Lee, Jehee; Oh, Byung‐Taek

doi:10.1016/j.micres.2017.09.003

Cited by 30 publications

(13 citation statements)

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“…The latter described that Pseudomonas strains were able to produce appreciable amounts of cellulase in culture-free supernatants and inhibited the mycelial growth of both pathogenic fungi Pythium aphanidermatum and Rhizoctonia solani. In addition, our results are in complete agreement with the findings of Durairaj et al [50] who reported no cellulase activity in the potent bacterial strains B. stratosphericus FW3 and Pseudomonas aeruginosa D4.…”

Section: Discussionsupporting

confidence: 93%

The Potential of Novel Bacterial Isolates from Natural Soil for the Control of Brown Rot Disease (Monilinia fructigena) on Apple Fruits

et al. 2020

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Monilinia fructigena is one of the most important fungal pathogens causing brown rot on apple and is heavily affecting fruit production. The main objective of this study was to screen for potential bacterial isolates with higher antagonistic activity against M. fructigena. Our study focused on the identification of potential bacterial isolates capable of reducing both the mycelial growth of M. fructigena and the disease severity using in vitro and in planta trials, respectively. To achieve this goal, thirteen bacteria, isolated from natural soil, were evaluated for their abilities to produce lytic enzymes (amylase, cellulase and protease), hydrocyanic acid (HCN) and lipopeptides (bacillomycin, fengycin, iturin and surfactin). Further, results from the dual culture method, volatile and bacterial free-cell filtrate bioassays indicated that tested isolates showed a fungicidal activity against the mycelial growth of M. fructigena. Thus, out of the 13 isolates tested, 12 exhibited significant mycelial inhibition (more than 70%) against M. fructigena, while remaining the last isolate displayed only a partial inhibition (up to 43%). Further, 12 of the bacteria isolates displayed an amylase production, 10 showed cellulase production, 11 revealed protease production, while only 2 displayed HCN production. In addition, most bacterial isolates were found to have genes encoding for different lipopeptides: bacillomycin (10), fengycin (3), iturin (11) and surfactin (1). Interestingly, two bacterial isolates, Bacillus amyloliquefaciens B10W10 and Pseudomonas sp. B11W11 were found to be the most effective and displayed the lowest disease severity in planta trial. These two bacteria reduced the brown rot incidence compared to the synthetic fungicide in a semi-commercial large-scale trial. Therefore, our findings suggest that these two later bacterial isolates provide apple protection against M. fructigena via direct and indirect mechanisms. These isolates may be used, therefore, as potential biological control agents (BCAs) in preventive treatment to control brown rot disease on apple fruits.

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Section: Discussionsupporting

confidence: 93%

The Potential of Novel Bacterial Isolates from Natural Soil for the Control of Brown Rot Disease (Monilinia fructigena) on Apple Fruits

et al. 2020

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“…Considering that the leishmaniasis treatment with pentavalent antimonials is known to be ineffective and unsafe and therapies based on pentamidine and amphotericin B are considered toxic and exhibit recurrence rates, it is clear that new treatment alternatives are necessary [132][133][134][135][136]. Although widely described as a producer of antibacterial and antifungal compounds [41,42,[137][138][139], we emphasize that the Burkholderia species' antileishmanial activity was unknown so far. As we demonstrated in this study, Burkholderiales may offer promising candidates to treat neglected diseases of which resistance and toxicity of current treatments represent a global public health concern.…”

Section: Discussionmentioning

confidence: 99%

Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

Cruz

Paula

Nogueira

et al. 2021

International Journal of Microbiology

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In this study, we report the isolation and identification of an endophytic strain of Burkholderia cepacia (COPS strain) associated with Polygala paniculata roots. Polygala plants are rich sources of promising microbiomes, of which the literature reports several pharmacological effects, such as trypanocidal, antinociceptive, anesthetic, anxiolytics, and anticonvulsant activities. B. cepacia COPS belongs to a new sequence type (ST 1870) and harbors a genome estimated in 8.3 Mbp which exhibits the aminoglycosides and beta-lactams resistance genes aph(3′)-IIa and blaTEM-116, respectively. Analysis performed using MLST, average nucleotide identity, and digital DNA-DNA hybridization support its species-level identification and reveals its novel housekeeping genes alleles gyrB, lepA, and phaC. The root endophyte B. cepacia COPS drew our attention from a group of 14 bacterial isolates during the primary screening for being potentially active against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Micrococcus luteus ATCC 9341, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 and exhibited the broad-spectrum activity against phytopathogenic fungi. In addition, COPS strain showed production of protease, lipase, and esterase in solid media, and its natural product extract showed potent inhibition against fungal plant pathogens, such as Moniliophthora perniciosa, whose antagonism index (89.32%) exceeded the positive control (74.17%), whereas Sclerotinia sclerotiorum and Ceratocystis paradoxa showed high percentages of inhibition (85.53% and 82.69%, respectively). COPS crude extract also significantly inhibited S. epidermidis ATCC 35984, E. faecium ATCC 700221 (MIC values of 32 μg/mL for both), E. faecalis ATCC 29212 (64 μg/mL), and S. aureus ATCC 25923 (128 μg/mL). We observed moderate antagonistic activity against A. baumannii ATCC 19606 and E. coli ATCC 25922 (both at 512 μg/mL), as well as potent cytotoxic effects on Leishmania infantum and Leishmania major promastigote forms with 78.25% and 57.30% inhibition. In conclusion, this study presents for the first time the isolation of an endophytic B. cepacia strain associated with P. paniculata and enough evidence that these plants may be considered a rich source of microbes for the fight against neglected diseases.

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“…carotovorum cells. Bacillus species have been used as a biocontrol agent against different pathogenic fungi (Mates et al 2019) and bacteria (Azaiez et al 2018;Durairaj et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Exploring rhizosphere and potato microbiome as potential antagonist to control blackleg and potato soft rot diseases in Morocco

Idrissi

Ouarzane

Elouazni

et al. 2021

Egypt J Biol Pest Control

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Background Blackleg and tuber soft rot are among the most important potato diseases caused by the bacteria belonging to the genera Pectobacterium. This pathogen causes significant economic losses each year. The antagonistic activity of different bacterial cultures against this pathogen was studied. Results Six hundred eight bacterial cultures isolated from potato tubers and rhizosphere soils procured from different locations across Morocco were tested for their antagonistic activity against Pectobacterium carotovorum. Forty isolates, all originating from tubers, showed positive antagonistic activity during preliminary screening. Among the 40 isolates, 10 were found to have a symptom suppression superior to 90%. Of the 10 isolates, 9 showed clear zone in the agar medium (in vitro test), with differences between antagonist’s inhibition diameter. For the in vivo test, 8 isolates induced total suppression of soft rot on potato slices (in vivo test). The other 2 biocontrol strains (Amo-23 and Atd-2) were capable to minimize soft rot symptoms of up to 94.4 and 96.2%, respectively. Among the selected strains for in planta experiment, 6 strains (namely Ame-4, Atd-2, Atd-4, Ag-216, Al-51, and Ama-501) showed total reduction of disease symptoms. Biochemical and molecular tests identified 8 strains of Bacillus sp. and 2 strains of Pseudomonas sp. Conclusions The results of the in vivo and the greenhouse experiments indicated that the selected isolates had a greatly significant effectiveness for suppressing blackleg and soft rot symptoms. The selected isolates could, therefore, be used as a biocontrol agent against blackleg and soft rot of potato.

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Characterization and assessment of two biocontrol bacteria against Pseudomonas syringae wilt in Solanum lycopersicum and its genetic responses

Cited by 30 publications

References 50 publications

The Potential of Novel Bacterial Isolates from Natural Soil for the Control of Brown Rot Disease (Monilinia fructigena) on Apple Fruits

The Potential of Novel Bacterial Isolates from Natural Soil for the Control of Brown Rot Disease (Monilinia fructigena) on Apple Fruits

Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

Exploring rhizosphere and potato microbiome as potential antagonist to control blackleg and potato soft rot diseases in Morocco

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