Characterization and applications of Thermomonas hydrothermalis isolated from Jordan's hot springs for biotechnological and medical purposes

Al-Daghistani, Hala I.; Mohammad, Balsam T.; Kurniawan, Tonni Agustiono; Singh, Deepak; Rabadi, Alexander; Xue, Wenchao; Avtar, Ram; Othman, Mohd Hafiz Dzarfan; Shirazian, Saeed

doi:10.1016/j.procbio.2021.03.010

Cited by 19 publications

(10 citation statements)

References 47 publications

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“…S9 harbored the highest abundance of these genes, corroborating with findings that this genus is often associated with warm to hot environments [ 118 ]. In fact, one of seven current members of the genus, the species T. hydrothermalis , has been commonly isolated from hot springs and used as a source of thermostable enzymes [ 119 , 120 ]. In contrast, the genera Cellulomonas and Paenibacillus encompass many species and their appearance is almost ubiquitous, including thermal springs [ 121 , 122 ].…”

Section: Discussionmentioning

confidence: 99%

Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis

Smrhova

Jani

Pajer

et al. 2022

Environmental Microbiome

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Background The extreme conditions of thermal springs constitute a unique aquatic habitat characterized by low nutrient contents and the absence of human impacts on the microbial community composition. Thus, these springs may host phylogenetically novel microorganisms with potential use in biotechnology. With this hypothesis in mind, we examined the microbial composition of four thermal springs of the world-renowned spa town of Karlovy Vary (Carlsbad), Czechia, which differ in their temperature and chemical composition. Results Microbial profiling using 16S rRNA gene sequencing revealed the presence of phylogenetically novel taxa at various taxonomic levels, spanning from genera to phyla. Many sequences belonged to novel classes within the phyla Hydrothermae, Altiarchaeota, Verrucomicrobia, and TA06. Cultivation-based methods employing oligotrophic media resulted in the isolation of 44 unique bacterial isolates. These include strains that withstand concentrations of up to 12% NaClw/v in cultivation media or survive a temperature of 100 °C, as well as hitherto uncultured bacterial species belonging to the genera Thermomonas, Paenibacillus, and Cellulomonas. These isolates harbored stress response genes that allow them to thrive in the extreme environment of thermal springs. Conclusions Our study is the first to analyze the overall microbial community composition of the renowned Karlovy Vary thermal springs. We provide insight into yet another level of uniqueness of these springs. In addition to their unique health benefits and cultural significance, we demonstrate that these springs harbor phylogenetically distinct microorganisms with unusual life strategies. Our findings open up avenues for future research with the promise of a deeper understanding of the metabolic potential of these microorganisms.

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Section: Discussionmentioning

confidence: 99%

Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis

Smrhova

Jani

Pajer

et al. 2022

Environmental Microbiome

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“…They included CaO (2.1% (w/w), K2O (6.7% (w/w)), Fe2O3 (4.3% (w/w)), and MgO (1.9% (w/w)). Their presence might contribute to Cr adsorption during water treatment [51]. 28.040°, 29.885°, 31.861°, 32.580°, 36.451°, and 50.051°, as confirmed by the JCPDS card (0 079-1460).…”

Section: Characterization Of Clinoptilolite Before and After Pretreat...mentioning

confidence: 70%

“…They included CaO (2.1% (w/w), K2O (6.7% (w/w)), Fe2O3 (4.3% (w/w)), and Mg (1.9% (w/w)). Their presence might contribute to Cr adsorption during water treatme [51]. Figure 3 shows the existence of spectra related to water molecules at 3400 and 1640 cm −1 .…”

Section: Characterization Of Clinoptilolite Before and After Pretreat...mentioning

confidence: 99%

Chromium Removal from Aqueous Solution Using Natural Clinoptilolite

Kurniawan

Othman

Adam

et al. 2023

Water

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This work investigates the applicability of clinoptilolite, a natural zeolite, as a low-cost adsorbent for removing chromium from aqueous solutions using fixed bed studies. To improve its removal performance for the inorganic pollutant, the adsorbent is pretreated with NaCl to prepare it in the homoionic form of Na+ before undertaking ion exchange with Cr3+ in aqueous solution. This work also evaluates if treated effluents could meet the required effluent discharge standard set by legislation for the target pollutant. To sustain its cost-effectiveness for wastewater treatment, the spent adsorbent is regenerated with NaOH. It was found that the clinoptilolite treated with NaCl has a two-times higher Cr adsorption capacity (4.5 mg/g) than the as-received clinoptilolite (2.2 mg/g). Pretreatment of the clinoptilolite with NaCl enabled it to treat more bed volume (BV) (64 BV) at a breakthrough point of 0.5 mg/L of Cr concentration and achieve a longer breakthrough time (1500 min) for the first run, as compared to as-received clinoptilolite (32 BV; 250 min). This suggests that pretreatment of clinoptilolite with NaCl rendered it in the homoionic form of Na+. Although pretreated clinoptilolite could treat the Cr wastewater at an initial concentration of 10 mg/L, its treated effluents were still unable to meet the required Cr limit of less than 0.05 mg/L set by the US Environmental Protection Agency (EPA).

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“…The presence of these probiotics in the present study may be beneficial to the nutrient availability, antioxidant and immune response of white shrimp [ 91 ]. Increased Aeromicrobium , Pseudonocardia , Thermomonas and Lysobacter , which could produce secondary metabolites and have antibacterial properties, may protect shrimp from pathogens [ 92 , 93 , 94 , 95 ]. Moreover, the bacteria Aquimarina , Tenacibaculum , Flavobacterium , Paeniclostridium , Escherichia-Shigella , Sulfurovum and Leucothrix are opportunistic or true pathogens that cause diseases of aquatic animals and egg mortality [ 96 , 97 , 98 , 99 ].…”

Section: Discussionmentioning

confidence: 99%

The Assessment of Dietary Organic Zinc on Zinc Homeostasis, Antioxidant Capacity, Immune Response, Glycolysis and Intestinal Microbiota in White Shrimp (Litopenaeus vannamei Boone, 1931)

et al. 2022

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This study aimed to assess dietary organic zinc on zinc homeostasis, antioxidant capacity, immune response, glycolysis and intestinal microbiota in white shrimp (Litopenaeus vannamei Boone, 1931). Six experimental diets were formulated: Control, zinc free; S120, 120 mg·kg−1 zinc from ZnSO4·7H2O added into control diet; O30, O60, O90 and O120, 30, 60, 90 and 120 mg·kg−1 zinc from Zn-proteinate added into control diet, respectively. The results showed that organic zinc significantly promoted zinc content and gene expression of ZnT1, ZIP11 and MT in the hepatopancreas and enhanced antioxidant capacity and immunity (in terms of increased activities of T-SOD, Cu/Zn SOD, PO, LZM, decreased content of MDA, upregulated expressions of GST, G6PDH, ProPO, LZM and Hemo, and increased resistance to Vibrio parahaemolyticus). Organic zinc significantly upregulated GluT1 expression in the intestine, increased glucose content of plasma and GCK, PFK and PDH activities of hepatopancreas, and decreased pyruvate content of hepatopancreas. Organic zinc improved intestinal microbiota communities, increased the abundance of potentially beneficial bacteria and decreased the abundance of potential pathogens. Inorganic zinc (S120) also had positive effects, but organic zinc (as low as O60) could achieve better effects. Overall, organic zinc had a higher bioavailability and was a more beneficial zinc resource than inorganic zinc in shrimp feeds.

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Characterization and applications of Thermomonas hydrothermalis isolated from Jordan's hot springs for biotechnological and medical purposes

Cited by 19 publications

References 47 publications

Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis

Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis

Chromium Removal from Aqueous Solution Using Natural Clinoptilolite

The Assessment of Dietary Organic Zinc on Zinc Homeostasis, Antioxidant Capacity, Immune Response, Glycolysis and Intestinal Microbiota in White Shrimp (Litopenaeus vannamei Boone, 1931)

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