Characterization and application of Raman labels for confocal Raman microspectroscopic detection of cellular proteins in single cells

Nithipatikom, Kasem; McCoy, Michael; Hawi, Sharon R.; Nakamoto, Kazuo; Adar, Fran; Campbell, William B.

doi:10.1016/j.ab.2003.07.020

Cited by 42 publications

(36 citation statements)

References 50 publications

(44 reference statements)

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“…One way in which the complexity of protein SERS can be avoided is to use SERS labels which are conjugated to biomolecules or biotin. In this way, specific cellular proteins or environments can be targeted using the SERS label (such as Cresyl violet), then incubated with silver colloid and then the SERS signal from the label attached to the protein detected [34]. This method has the obvious advantage that it can avoid non-specific interactions that can hinder SERS, and that the SERS signal will be very well defined, thus enhancing reproducibility.…”

Section: Sers Of Large Biological Moleculesmentioning

confidence: 99%

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

Ryder

2005

Current Opinion in Chemical Biology

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Raman spectroscopy is rapidly finding favour for applications in the life science due to the ease with which it can be used to extract significant data from tissue and cells. However, the Raman effect is an inherently weak effect, which hinders the analysis of low concentration analytes. The Raman sensitivity can be improved via the Surface Enhanced Raman Scattering (SERS) effect. In SERS, Raman spectra are dramatically increased when a molecule is adsorbed onto nanoroughened noble metal surfaces such as silver and gold. The degree of enhancement enables single molecule detection, which offers the potential for the unambiguous identification of analytes at concentrations that are useful in both a forensic and a chemical biology context. Here we discuss some of the practical applications of SERS to both low-level narcotic detection, and how this can be applied to chemical biology.

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Section: Sers Of Large Biological Moleculesmentioning

confidence: 99%

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

Ryder

2005

Current Opinion in Chemical Biology

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“…CV was chosen as the Raman reporter owing to its large SERS enhancement as previously described. 39 Typically, the labeling was carried out as follows: 2 µl of 1 mM CV and 100 µl of EDC solution (10 mg dissolved in 1 ml of ultrapure H 2 O) were added to 100 µl of MAA-labeled silver colloids in MES buffer and allowed to react for 24 h. The reporterlabeled silver colloids were separated from the solution by centrifugation. After four rinses, the silver sediments were resuspended in 100 µl of sterile ultra-pure water and stored at 4°C.…”

Section: Synthesis Of Raman Dye-labeled Silver Nanoparticlesmentioning

confidence: 99%

Detection of Raman Scattering Spectra of High Spectral Resolution in Short Oligonucleotides: Compared with the Full-Length DNA Spectra

et al. 2017

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We present recent development and applications of a surface-enhanced Raman scattering (SERS) technology for use in medical diagnostics and biological imaging. For medical diagnostics, we use Raman-active dye-labeled DNA gene probes and nanostructured metallic substrates as SERS-active platforms. The surface-enhanced Raman gene probes can be used to detect DNA biotargets (e.g. gene sequences, bacteria and viral DNA) via hybridization to DNA sequences complementary to these probes. The SERS gene probes eliminate the need for radioactive labels and have great potential to provide both sensitivity, selectivity and label multiplexing for DNA sequencing and clinical assays. We also describe a hyperspectral surface-enhanced Raman imaging (HSERI) system that combines imaging capabilities with SERS detection to identify cellular components with high spatial and temporal resolution. The HSERI system's application to biological imaging is demonstrated using Raman dye-labeled silver nanoparticles in cellular systems.

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“…79 SERS-confocal microscopy has been used in chemical imaging of cellular proteins, including enzymes, cellular proteins, and receptors in single cells. 80 Surfaceenhanced micro-Raman spectroscopy in model diagnostic systems incorporating microfluidics 81 showed potentially promising application in pharmaceutical research and clinical chemistry. Bacterial discrimination 82 via SEM-SERS, and viral detection in immunoassays 83 and DNA nanoimaging 84 via SERS-AFM, have been recently reported.…”

Section: Surface-enhanced Raman Spectroscopymentioning

confidence: 99%

Raman characterization and chemical imaging of biocolloidal self-assemblies, drug delivery systems, and pulmonary inhalation aerosols: A review

Mansour

Hickey

2007

AAPS PharmSciTech

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This review presents an introduction to Raman scattering and describes the various Raman spectroscopy, Raman microscopy, and chemical imaging techniques that have demonstrated utility in biocolloidal self-assemblies, pharmaceutical drug delivery systems, and pulmonary research applications. Recent Raman applications to pharmaceutical aerosols in the context of pulmonary inhalation aerosol delivery are discussed. The "molecular fingerprint" insight that Raman applications provide includes molecular structure, drug-carrier/ excipient interactions, intramolecular and intermolecular bonding, surface structure, surface and interfacial interactions, and the functional groups involved therein. The molecular, surface, and interfacial properties that Raman characterization can provide are particularly important in respirable pharmaceutical powders, as these particles possess a higher surface-area-to-volume ratio; hence, understanding the nature of these solid surfaces can enable their manipulation and tailoring for functionality at the nanometer level for targeted pulmonary delivery and deposition. Moreover, Raman mapping of aerosols at the micro-and nanometer level of resolution is achievable with new, sophisticated, commercially available Raman microspectroscopy techniques. This noninvasive, highly versatile analytical and imaging technique exhibits vast potential for in vitro and in vivo molecular investigations of pulmonary aerosol delivery, lung deposition, and pulmonary cellular drug uptake and disposition in unfixed living pulmonary cells.

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Characterization and application of Raman labels for confocal Raman microspectroscopic detection of cellular proteins in single cells

Cited by 42 publications

References 50 publications

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

Detection of Raman Scattering Spectra of High Spectral Resolution in Short Oligonucleotides: Compared with the Full-Length DNA Spectra

Raman characterization and chemical imaging of biocolloidal self-assemblies, drug delivery systems, and pulmonary inhalation aerosols: A review

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