Characterization and Antimicrobial Activity of Alkaloid Extracts from Seeds of Different Genotypes of Lupinus spp.

Romeo, Flora V.; Fabroni, Simona; Ballistreri, Gabriele; Muccilli, Serena; Spina, Alfio; Rapisarda, Paolo

doi:10.3390/su10030788

Cited by 35 publications

(47 citation statements)

References 34 publications

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“…Sweet lupine extract showed no inhibition against neither E. coli nor S. aureus bacteria. The negative results reported here are in agreement with previous studies in terms of E. coli, but it does not agree with the results of the study on S. aureus, where significant activity was observed [16,17]. The extract of Lupinus angustifolius was weakly active on E. coli.…”

Section: Antimicrobial Activitycontrasting

confidence: 56%

Determination of Minerals, Total Phenolic Content, Flavonoids, Antioxidants and Antimicrobial Activities of Ethanolic Extract of Sweet Lupinus angustifolius of Palestine

Hanania

Radwan

Odeh

et al. 2019

EJMP

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Aims: To establish the most suitable extraction method for sweet lupine seeds and to determine minerals, phenolic content, flavonoids, antioxidant activity and antimicrobial activities. Study Design: Known and standard experimental procedures are employed. Place and Duration of Study: Department of Chemistry, Bethlehem University- Palestine, from January 2019 to March 2019. Methodology: Seeds were ground and extracted by Soxhlet extractor using ethanol with different percentages (50%, 60%, 70%, 80% and 95%). Sodium, potassium and ferrous ion content were determined. Resistance to bacteria was performed against Escherichia coli and Staphylococcus aureus, while antioxidant activity was determined by FRAP method. Two types of flavonoids were measured: Flavonones and dihydroflavonols via the reaction with 2,4-dinitrophenylhydrazine. Phenolics were determined by the Folin-Ciocalteu method. Results: 50% ethanol resulted in the highest extract residue (18.6%) while 70% and 60% showed the lowest content (10.0% for both). 80% ethanol extracted sample showed the highest content for sodium (56.51 mg Na/g extract), while 60% and 50% ethanol extracts showed the highest content of potassium (2.25 and 2.33 mg K/g extract, respectively). The maximum concentration of ferrous ion was obtained with 70% ethanol (6.854 mg Fe+2/g extract). 95% ethanolic extract showed the highest antioxidant activity (20.24 mg FeSO4/g extract). Similar results were obtained for total phenolic content and flavonoids: 24.60 mg gallic acid/g extract for phenolics and 116.02 mg rutin/g extract for flavonoids. Extracts showed no bacterial activity against both types of bacteria used. Conclusion: 95% ethanol extracted samples showed the highest antioxidant activity and the highest flavonoids and phenolic content. Sweet lupine extract did not perform any antimicrobial activity against both Gram positive and Gram negative bacteria.

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Section: Antimicrobial Activitycontrasting

confidence: 56%

Determination of Minerals, Total Phenolic Content, Flavonoids, Antioxidants and Antimicrobial Activities of Ethanolic Extract of Sweet Lupinus angustifolius of Palestine

Hanania

Radwan

Odeh

et al. 2019

EJMP

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“…Widely distributed Antioxidant and antiinflammatory properties, lipid peroxidation, DNA cleavage protection (Acquaviva et al, 2003;Pietta et al, 2003;Rossi et al, 2012) Terpenoids and steroid Triterpenoid saponins Saponins Chickpea, soybean, lentils, peanut, common bean, and alfalfa sprouts Reduction of cholesterol content, antimicrobial and anti-cancer properties (Shi et al, 2004(Shi et al, , 2014Hassan et al, 2010;Man et al, 2010;Marrelli et al, 2016) Tetraterpenes Carotenoids Widely distributed Antioxidant, better visual function, reduction of cardiovascular diseases (Voutilainen et al, 2006;Roberts et al, 2009) Alkaloids Quinolizidine alkaloids (QA) Sparteine Lupinus spp. Antimicrobial properties (Romeo et al, 2018)…”

Section: The Health Benefits Of Secondary Metabolites From Legumesmentioning

confidence: 99%

The Effects of Domestication on Secondary Metabolite Composition in Legumes

Contador

et al. 2020

Front. Genet.

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Legumes are rich in secondary metabolites, such as polyphenols, alkaloids, and saponins, which are important defense compounds to protect the plant against herbivores and pathogens, and act as signaling molecules between the plant and its biotic environment. Legume-sourced secondary metabolites are well known for their potential benefits to human health as pharmaceuticals and nutraceuticals. During domestication, the color, smell, and taste of crop plants have been the focus of artificial selection by breeders. Since these agronomic traits are regulated by secondary metabolites, the basis behind the genomic evolution was the selection of the secondary metabolite composition. In this review, we will discuss the classification, occurrence, and health benefits of secondary metabolites in legumes. The differences in their profiles between wild legumes and their cultivated counterparts will be investigated to trace the possible effects of domestication on secondary metabolite compositions, and the advantages and drawbacks of such modifications. The changes in secondary metabolite contents will also be discussed at the genetic level to examine the genes responsible for determining the secondary metabolite composition that might have been lost due to domestication. Understanding these genes would enable breeding programs and metabolic engineering to produce legume varieties with favorable secondary metabolite profiles for facilitating adaptations to a changing climate, promoting beneficial interactions with biotic factors, and enhancing health-beneficial secondary metabolite contents for human consumption.

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“…Although 13-hydroxylupanine, 17-oxosparteine and 11, 12-dehydrosparteine have been described in L. mutabilis [16,17], they were not identified in the ecotypes investigated in our research. Some of the alkaloids detected are common in other species too, e.g., angustifoline, lupanine and sparteine in L. albus seeds, sparteine in L. luteus, lupanine in L. hispanicus, and angustifoline in L. angustifolius [23][24][25][26][27]. It is worth noting that alkaloids such as α-piridone, cytisine, and anagyrine, which are highly poisonous for invertebrates and common in wild species [23], were not found in the samples of L. mutabilis.…”

Section: Alkaloid Profile In Bitter Seedsmentioning

confidence: 99%

Profile and Content of Residual Alkaloids in Ten Ecotypes of Lupinus mutabilis Sweet after Aqueous Debittering Process

Cortés-Avendaño

Tarvainen

Suomela

et al. 2020

Plant Foods Hum Nutr

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The evaluation of the level of alkaloids in edible Lupinus species is crucial from a food safety point of view. Debittering of lupin seeds has a long history; however, the control of the level of alkaloids after processing the seeds is typically only evaluated by changes in the bitter taste. The aim of this study was to evaluate the profile and residual levels of quinolizidine alkaloids (QA) in (Lupinus mutabilis Sweet) after aqueous debittering process. Samples from 10 ecotypes from different areas of Peru were analyzed before and after the process. Based on results obtained by gas chromatography and mass spectrometry, from eight alkaloids identified before the debittering process, only small amounts of lupanine (avg. 0.0012 g/100 g DM) and sparteine (avg. 0.0014 g/100 g DM) remained in the seeds after the debittering process, and no other alkaloids were identified. The aqueous debittering process reduced the content of alkaloids to levels far below the maximal level allowed by international regulations (≤ 0.2 g/kg DM).

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Characterization and Antimicrobial Activity of Alkaloid Extracts from Seeds of Different Genotypes of Lupinus spp.

Abstract: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA),

Cited by 35 publications

References 34 publications

Determination of Minerals, Total Phenolic Content, Flavonoids, Antioxidants and Antimicrobial Activities of Ethanolic Extract of Sweet Lupinus angustifolius of Palestine

Determination of Minerals, Total Phenolic Content, Flavonoids, Antioxidants and Antimicrobial Activities of Ethanolic Extract of Sweet Lupinus angustifolius of Palestine

The Effects of Domestication on Secondary Metabolite Composition in Legumes

Profile and Content of Residual Alkaloids in Ten Ecotypes of Lupinus mutabilis Sweet after Aqueous Debittering Process

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