Characterization and antibacterial activity of endophytic fungi isolated from Avicennia marina

Trivedi, Nidhi S.; Thumar, Jignasha T.

doi:10.1007/s42535-023-00582-9

Cited by 2 publications

(3 citation statements)

References 35 publications

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“…The Aspergillus sp., KR-1 10( 14), used in the current study, was isolated from the root of Avicennia marina derived from the Kutch region of Gujarat, India (23 • 01'58.6" N, 70 • 09'27.3" E). Molecular identification of the fungi was conducted, and the resulting ITS sequences were submitted to NCBI GenBank under the accession number MW447519 (13) .…”

Section: Identification Of Fungimentioning

confidence: 99%

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Statistical Optimization for the Production of Antimicrobial Compounds Produced by Mangrove Endophytic Aspergillus sp.

Trivedi¹,

Thumar²

2023

IJST

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Objectives:The purpose of this study was to investigate the antibacterial properties of an endophytic fungus, Aspergillus sp., isolated from the roots of the Avicennia marina belonging to an unexplored location in Kutch, Gujarat, India. The study aimed to optimize various factors to enhance the production of antimicrobial compounds. Methodology: Antimicrobial activity was performed using the agar-well diffusion method, and the results were compared with the standard antibiotic streptomycin as a positive control and DMSO as a negative control. The classical one-factor-at-a-time (OFT) approach was used to optimize various factors such as temperature, pH, culture media, carbon and nitrogen sources, and solvents to enhance antimicrobial production. Response Surface Methodology (RSM) was used to statistically optimize the production of antimicrobial compounds. Findings: The investigation revealed that the endophyte exhibited remarkable inhibitory action against sensitive strains of Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus. The most suitable conditions for the production of antimicrobials were; an inoculum size of 1*10 5 in potato dextrose medium at a temperature of 25 • C and pH 6, with an incubation period of 12 days. Starch and ammonium chloride acted as the best carbon and nitrogen sources, respectively. The use of RSM resulted in a 1.46-fold increase in the antimicrobial activity against Pseudomonas sp., with an increase in the zone of inhibition from 18.36mm to 26.8mm. Novelty: The study highlights the unique antimicrobial potential of an unexplored endophyte derived from Avicennia marina roots in Kutch, Gujarat, India. RSM played a crucial role in detecting optimum conditions for the production of antimicrobial compounds, resulting in a significant increase in activity. Its application emphasizes the importance of advanced statistical techniques in improving our understanding of complex biological systems.

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Section: Identification Of Fungimentioning

confidence: 99%

“…The broth was filtered using Whatmann filter paper no. 1, and the resultant filtrate was subjected to antimicrobial analysis by agar-well diffusion assay (13) . Streptomycin sulphate and DMSO were used as positive and negative controls, respectively.…”

Section: Screening Of Antimicrobial Activity From Fungal Extractmentioning

confidence: 99%

Statistical Optimization for the Production of Antimicrobial Compounds Produced by Mangrove Endophytic Aspergillus sp.

Trivedi¹,

Thumar²

2023

IJST

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“…Research on endophytic fungi extracted from mangrove plants yielded encouraging results, indicating their potential to harbor novel metabolites that could significantly benefit their host organisms (Maria et al 2005). In a previous study conducted by Trivedi and Thumar strong antibacterial activity was reported for endophytic fungi isolated from the roots of A. marina, a mangrove species collected from the Gulf of Kutch in Gujarat, India (at coordinates 23°01′58.6"N 70°09′27.3"E) (Trivedi and Thumar 2023). As such, we embarked on a more extensive investigation into endophytic fungi isolated from the leaves, roots, and stems of A. marina, presenting a comprehensive overview of our findings.…”

Section: Introductionmentioning

confidence: 98%

Antibacterial activities and molecular identification of endophytic fungi isolated from mangrove Avicennia marina

MULYANI,

WULANDARI,

SINAGA

et al. 2024

Biodiversitas

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Abstract. Mulyani Y, Wulandari AP, Sinaga SE, Safriansyah W, Azhari A, Purbaya S, Abdullah FF, Farabi K, Shiono Y, Supratman U. 2023. Antibacterial activities and molecular identification of endophytic fungi isolated from mangrove Avicennia marina. Biodiversitas 24: 6923-6933. This research explored the antibacterial potential of endophytic fungi from the Avicennia marina's (Forssk.) Vierh leaves, stem bark, and root, located in Blanakan, Subang District, West Java. The screening process for antibacterial activity potential was conducted using the Kirby Bauer disk diffusion method. Subsequently, the molecular screening results of the most promising fungi species were identified using Internal Transcribed Spacer (ITS) markers. The results revealed the isolation of 30 fungal isolates. Among these, seven endophytic fungi exhibited significant antibacterial activity against Staphylococcus aureus ATCC 29213 and Vibrio harveyi ATCC 5339, with inhibition zones ranging from 7.88±1.52 to 23.60±0.77 mm. Through molecular identification, 7 endophytic fungi were identified, including Penicillium chrysogenum, Cladosporium anthropophilum, Trichosporon asahii, Cladosporium sphaerospermum, Fusarium verticillioides, Meyerozyma carpophila, and Penicillium steckii. Notably, C. anthropophilum exhibited the highest inhibition zones against S. aureus and V. harveyi measuring 23.60±0.77 and 21.80±0.26 mm, and showed (Minimum Inhibitory Concentration) MIC values of 15.625±0.98 and 31.25±0.39 ?g/mL, respectively. In this study, endophytic fungi isolated from different parts of A. marina exhibited promising antibacterial activity, with Cladosporium anthropophilum from the stem bark showing the highest potency against S. aureus and V. harveyi, suggesting their potential as a source of antibacterial agents. To the best of our knowledge, this is the inaugural study uncovering the isolation and antibacterial potential of endophytic fungi from the genera Cladosporium, Trichosporon, Fusarium, and Meyerozyma. These fungi were extracted from the bark, leaves, and roots of A. marina, situated within the unique mangrove ecosystem of Blanakan Sub-district, Subang District, West Java, Indonesia.

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Characterization and antibacterial activity of endophytic fungi isolated from Avicennia marina

Cited by 2 publications

References 35 publications

Statistical Optimization for the Production of Antimicrobial Compounds Produced by Mangrove Endophytic Aspergillus sp.

Statistical Optimization for the Production of Antimicrobial Compounds Produced by Mangrove Endophytic Aspergillus sp.

Antibacterial activities and molecular identification of endophytic fungi isolated from mangrove Avicennia marina

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