Characterization and anti-diabetic effects of the oligosaccharide fraction isolated from Rosa canina in STZ-Induced diabetic rats

Rahimi, Mehrali; Sajadimajd, Soraya; Mahdian, Zahra; Hemmati, Mohammad; Malekkhatabi, Peyman; Bahrami, Gholamreza; Mohammadi, Bahareh; Miraghaee, Shahram; Hatami, Razieh; Mansouri, Kamran; Motlagh, Hamid Reza Moahammadi; Keshavarzi, Samira; Derakhshankhah, Hossein

doi:10.1016/j.carres.2020.107927

Cited by 19 publications

(21 citation statements)

References 39 publications

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“…(abdominal pain), H. perforatum (wound and haemorrhoids), Juglans regia L. (haemorrhoids) , L. nobilis (rheumatism), M. sylvestris L. (abdominal pain), Mentha x piperita L. (abdominal pain), P. lanceolata L. (wounds), Punica granatum (diarrhea), Rosa canina L. (diabetes) and Urtica urens L. (rheumatism). Further studies on some of the listed species support the folkloric uses of these plants with new evidence: the wound-healing properties of P. brutia have been shown (Cetin et al, 2013); H. perforatum has been predominantly used for treating depression, wounds and ulcers ( EMA 2006 https://www.ema.europa ); Rosa canina has been studied for antidiabetic properties ( Rahimi et al, 2020 ); and the antihypertensive, anti-inflammatory and anti-ulcerogenic properties of Cydonia oblonga have been investigated ( Zhouet al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

An Ethnobotanical Study of Medicinal Plants in Mersin (Turkey)

et al. 2021

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This comprehensive ethnobotanical study carried out in Mersin province, which is located in the southern part of Anatolia, east of the Mediterranean Sea, compiles details on plants used in folk medicine and ethnopharmacological information obtained through face-to-face interviews. The aim was to collect and identify plants used for therapeutic purposes by local people and to record information on traditional herbal medicine. Plant specimens were collected in numerous excursions. Additionally, informant consensus factor and use value (UV) were calculated for information gathered. This study identifies 93 plant taxa belonging to 43 families and records their usage in folk medicine; 83 taxa are wild and the remaining 10 are cultivated. The most commonly used plants belong to Lamiaceae, representing 15.0% of the total, while the Rosaceae, Malvaceae, Hypericaceae, Asteraceae and Cupressaceae families each represented another 5.4%. As a result of this investigation, we determine 189 medicinal usages of 93 taxa. The UV values indicate that the most important medicine plants are Hypericum perforatum (0.80), Cedrus libani (0.78), Quercus coccifera (0.77), Arum dioscoridis (0.76) and Juniperus drupaceae (0.74). We observed that most of the drugs are prepared using the infusion method (27.6%). As a conclusion, the study finds that traditional folk medicine usage is still common, especially among the rural population of Mersin.

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Section: Discussionmentioning

confidence: 99%

An Ethnobotanical Study of Medicinal Plants in Mersin (Turkey)

et al. 2021

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“…In vitro study suggested that high concentrations of oligosaccharide increased cell death, while at low concentration protected beta-pancreatic cells from STZ-induced cell death. The anti-diabetic activity of this oligosaccharide was confirmed at the molecular level ( 10 ). Due to structural similarity between the Rosaceae family and the Rosa canina , we investigated the presence of any new oligosaccharide with anti-diabetogenic activity in the large family of Rosaceae.…”

Section: Introductionmentioning

confidence: 84%

“…During the purification step, the extract was precipitated by three times its volume of 96% ethanol and the resulting precipitate was dissolved in deionized water. The fractions containing the corresponding oligosaccharide were isolated according to the previous method described for the isolation of Rosa canina ( 10 ).…”

Section: Methodsmentioning

confidence: 99%

“…Our results confirmed that the isolated polysaccharide from Rosa canina can promote the proliferation of pancreatic beta cells providing a novel mechanism for the anti-diabetic effect of the plant ( 8 ). Also, we purified and evaluated a novel oligosaccharide with anti-diabetic activity from Rosa canina ( 9 10 11 ). We used high-performance liquid chromatography diode array detector tandem mass spectroscopy (HPLC-DAD MS/MS), infrared radiation (IR), and nuclear magnetic resonance (NMR) techniques and identified an oligosaccharide with a pectin structure composed of repeated tetrasaccharides ([C 29 H 42 O 26 ] n) and several acetyl and methoxycarbonyl groups ( Fig.…”

Section: Introductionmentioning

confidence: 99%

“…We used high-performance liquid chromatography diode array detector tandem mass spectroscopy (HPLC-DAD MS/MS), infrared radiation (IR), and nuclear magnetic resonance (NMR) techniques and identified an oligosaccharide with a pectin structure composed of repeated tetrasaccharides ([C 29 H 42 O 26 ] n) and several acetyl and methoxycarbonyl groups ( Fig. 1 ) ( 10 ). In vitro study suggested that high concentrations of oligosaccharide increased cell death, while at low concentration protected beta-pancreatic cells from STZ-induced cell death.…”

Section: Introductionmentioning

confidence: 99%

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Antidiabetic potential of the isolated fractions from the plants of Rosaceae family in streptozotocin-induced diabetic rats

Bahrami

Izadi

Miraghaee

et al. 2021

Research in Pharmaceutical Sciences

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Background and purpose: Diabetes is a group of multifactorial disorders characterized by chronic-elevated blood glucose levels (hyperglycemia). Natural remedies are used as alternative medications to treat diabetes. Here, we tested the protective effect of the plant extracts of the Rosaceae family on improving insulin secretion and repairing the pancreatic beta cells in diabetic rats. Experimental approach: The oligosaccharide fraction was isolated from the Rosaceae family of herbs. LC-MS/MS was applied to characterize the isolated fractions. The male Wistar rats were randomly divided into six groups, 10 each, including the control group with no intervention, diabetic rats without treatment, diabetic rats that received the extract of Malus domestica (apple), Cydonia oblonga (quince), Prunus persica (nectarine), and Prunus persica (peach), separately. Rats were monitored for the weight, fasting plasma glucose, and insulin levels. The effect of extracts in streptozotocin (STZ)-induced diabetic rats on the pancreatic islets was evaluated by morphometric analysis. Findings/Results: LC-MS/MS results indicated a similar mass spectrum of isolated fractions from nectarine and peach with Rosa canina . Oral administration of nectarine and peach extracts to STZ-induced diabetic rats showed restoration of blood glucose levels to normal levels with a concomitant increase in insulin levels. Morphometric analysis of pancreatic sections revealed the increase in number, diameter, volume, and area of the pancreatic islets in the diabetic rats treated with extracts compared to the untreated diabetic rats. Conclusion and implications: Nectarine and peach extracts’ anti-diabetic properties improved insulin secretion and pancreatic beta-cell function and subsequently led to restoring pancreatic islet mass in STZ-induced diabetic rats.

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Notch signaling‐induced cyclin d1 in diabetes ameliorating effects of the isolated polysaccharide from Rosa canina: In vitro and in vivo studies

Sajadimajd

Bahrami

Mohammadi

et al. 2022

Cell Biochemistry & Function

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Notch signaling has a role in the expansion of the pancreas and the pathogenesis of diabetes. Modulation of Notch signaling by natural products seems to pave the way for treating diabetes. This research aimed to scrutinize the involvement of the Notch cascade in the diabetes‐ameliorating effects of an isolated polysaccharide from Rosa canina. The isolated polysaccharide was characterized using Fourier transform infrared, nuclear magnetic resonance, high‐performance gel‐permeation chromatography, and liquid chromatography with tandem mass spectrometry techniques. Rat pancreatic β cells and STZ‐induced diabetic rats were treated with the isolated polysaccharide. MTT assay, cell cycle analysis, quantative realtime‐polymerase chain reaction, immunohistochemistry, and immunoblotting were used to reveal the growth and the expression levels of Notch1, DLL4, Jagged‐1, hes1, Ins‐1, Pdx‐1, and cyclin d1 in treated and untreated pancreatic cells and tissues. The ameliorating effect of the polysaccharide in STZ‐treated cells was accomplished by upregulation of cyclin d1 and hes1 as well as cell cycle progression. Notch inhibition by LY‐411575 was associated with the downregulation of cyclin d1 which upregulates with polysaccharide treatment. The significant expression of cyclin d1 (90%) and nuclear expression of hes1 in the pancreas of the polysaccharide group were accompanied by improvement of hyperglycemia and associated biochemical factors as well as regeneration of islet cells as compared to untreated diabetic rats. Based on these findings, upregulation of Notch signaling‐induced cyclin d1 could be proposed as the underlying diabetes‐reducing effects of the isolated polysaccharide derivative implying that cyclin d1 actuation through activation of the Notch‐DLL4 circuit may play the causal role in the treatment of diabetes.

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Characterization and anti-diabetic effects of the oligosaccharide fraction isolated from Rosa canina in STZ-Induced diabetic rats

Cited by 19 publications

References 39 publications

An Ethnobotanical Study of Medicinal Plants in Mersin (Turkey)

An Ethnobotanical Study of Medicinal Plants in Mersin (Turkey)

Antidiabetic potential of the isolated fractions from the plants of Rosaceae family in streptozotocin-induced diabetic rats

Notch signaling‐induced cyclin d1 in diabetes ameliorating effects of the isolated polysaccharide from Rosa canina: In vitro and in vivo studies

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