Characterization and anti-aging effects of <i>Opuntia ficus-indica</i> (L.) Miller extracts in a <scp>d</scp>-galactose-induced skin aging model

Abbas, Eman Yasser; Ezzat, Marwa I.; Ramadan, Nehal M.; Eladl, Amira; Hamed, Walaa H E; Abdel‐Aziz, Marwa M.; Teaima, Mahmoud H.; El-Hefnawy, Hala M; Abdel-Sattar, Essam

doi:10.1039/d2fo03834j

Cited by 5 publications

(1 citation statement)

References 109 publications

(154 reference statements)

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“…The BALB/c‐nu/nu mice were 6−8 weeks old, weighed 18–22 g, and consisted of an equal number of males and females. 36 The animals were subjected to a 1‐week‐long adaptation phase in regular enclosures. Regular residues were offered while minimizing the use of animals as much as possible.…”

Section: Methodsmentioning

confidence: 99%

Prinsepia utilis Royle polysaccharides promote skin barrier repair through the Claudin family

Wang,

et al. 2024

Skin Research and Technology

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BackgroundPlant polysaccharides have various biological activities. However, few studies have been conducted on the skin barrier of Prinsepia utilis Royle polysaccharide extract (PURP).Materials and methodsThe proportions of polysaccharides, monosaccharides and proteins were determined by extracting polysaccharides from fruit meal using water. The healing rate was measured by cell scratch assays. SDS‐damaged reconstructed human epidermal models, an acetone–ether‐induced mouse model and an IL‐4‐induced cellular inflammation model were used to detect the effects of polysaccharides on the phenotype, HA, TEWL, and TEER, with further characterizations performed using QRT‐PCR, Western blotting, immunofluorescence (IF) assays.ResultsPURP contained 35.73% polysaccharides and 11.1% proteins. PURP promoted cell migration and increased skin thickness in a reconstructed human epidermis model. The TEWL significantly decreased, and the HA content significantly increased. PURP significantly increased the TEER and decreased the permeability of the SDS‐damaged reconstructed human epidermis model. Claudin‐3, Claudin‐4, and Claudin‐5 were significantly upregulated. IF and Western blot analysis revealed that the Claudin‐4 level significantly increased after treatment with PURP. Claudin‐1, Claudin‐3, Claudin‐4, and Claudin‐5 gene expression and IF and immunohistochemical staining were significantly increased in mice treated with acetone–ether. PURP promoted the expression of Claudin‐1, Claudin‐3, Claudin‐4, and Claudin‐5 after treatment with 100 ng/mL IL‐4. PURP also downregulated the expression of NO, IL6, TNFα and NFκB in Raw 264.7 cells and in a mouse model.ConclusionWe hypothesize that PURP may repair the skin barrier by promoting the expression of the claudin family and can assist in skin therapy.

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Section: Methodsmentioning

confidence: 99%