Characterization and androgenic regulation of major mRNAs coding for epididymal proteins in a lizard (Lacerta vivipara)

Courty, Yves; Morel, Frédéric; Dufaure, Jean-Pierre

doi:10.1530/jrf.0.0810443

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…Most evidence to date suggests that androgens control protein synthesis by regulating gene expression at transcriptional and/or post-transcriptional levels (D'Agostino et al, 1980;Brooks et al, 1986a, b;Mord et al, 1986;Brooks, 1987a;Courty et al, 1987). The mechanism for the mouse epididymis is not known although several androgen-dependent proteins have been identified (Flickinger et al, 1986;Holland & Orgebin-Crist, 1988), amongst which some bind specifically to the surface of spermatozoa (Devine & Carroll, 1984;Murphy & Carroll, 1984Lakoski et al, Carroll, 1985).…”

Section: Introductionmentioning

confidence: 99%

Androgen-dependent messenger RNA(s) related to secretory proteins in the mouse epididymis

Ghyselinck¹,

Jimenez²,

Courty³

et al. 1989

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Total RNA from mouse epididymides was translated in a cell-free system derived from rabbit reticulocyte lysate. The androgen dependence of a highly represented mRNA(s) was detected. This mRNA(s) encoded for a band of Mr 26,000 visualized by denaturing gel electrophoresis (SDS-PAGE). No other mouse sexual tissues (testis, vas deferens and seminal vesicle), liver, kidney or striated muscle presented such a band. Furthermore, this mRNA(s) was restricted to the caput epididymidis. Two-dimensional gel electrophoresis (2D-PAGE) showed that this band of Mr 26,000 was composed of 6 basic polypeptides. This translated protein may correspond to a newly synthesized secretory protein of Mr 24,000. The concentration of translatable mRNA(s) encoding for the band of Mr 26,000 dropped to 31% of the normal level at 3 days after castration and to 7%, 20 days later. Administration of testosterone to 30-day-castrated mice partly reversed these changes: 32% of the control value was reached after 10 days of treatment. Accumulation of this mRNA(s) was also just detectable (7% of the mature level) at 10 days of age. The high levels attained by 20 days of age (60% of the control value) suggest that the greatest increase of accumulation for this mRNA(s) occurs during the prepubertal period.

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Section: Introductionmentioning

confidence: 99%

Androgen-dependent messenger RNA(s) related to secretory proteins in the mouse epididymis

Ghyselinck¹,

Jimenez²,

Courty³

et al. 1989

Reproduction

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“…It is composed of 9 elements (LESP I to LESP IX) that exhibit an identical 18 kDa molecular mass, but a wide heterogeneity of pHi, ranging from 3·5 to 9 (Depeiges et al 1988). These LESP elements, whose synthesis is under androgenic control (Depeiges et al 1981, Courty et al 1987, are secreted in the epididymal lumen where they mix and bind to the spermatozoa (Depeiges & Dufaure 1983). According to these observations and in the light of previous studies (Depeiges & Dacheux 1985), it has been proposed that the LESPs play a role in sperm maturation in the lizard epididymal fluid.…”

Section: Introductionmentioning

confidence: 99%

The lipocalin sperm coating lizard epididymal secretory protein family: mRNA structural analysis and sequential expression during the annual cycle of the lizard, Lacerta vivipara

Morel¹,

Dufaure²,

Depeiges³

2000

Journal of Molecular Endocrinology

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The epididymal epithelial cells of the lizard (Lacerta vivipara) produce large amounts of specific proteins under androgenic control. Amongst them, a major protein family that binds to the head of spermatozoa, the lizard epididymal secretory protein (LESP) family, has been identified as a member of the lipocalin superfamily. LESPs are composed of 9 elements that present an identical molecular mass of 18 000 Da but have a large range of pHi (3·5 to 9). The structural analysis of this protein family was performed by the determination and comparison of both the aminoterminal sequence of each element and the complete sequence of three specific LESP cDNA clones. When not identical, LESP elements present randomly dispatched nucleotide and amino acid substitutions, indicating the existence of at least five LESP mRNA populations encoded by a multigenic family. We determined that these LESP genes are differentially expressed during the annual epididymal cycle.

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Regulation of the epididymal glutathione peroxidase-like protein in the mouse: Dependence upon androgens and testicular factors

Rigaudière

Ghyselinck

Faure

et al. 1992

Molecular and Cellular Endocrinology

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Characterization and androgenic regulation of major mRNAs coding for epididymal proteins in a lizard (Lacerta vivipara)

Cited by 8 publications

References 23 publications

Androgen-dependent messenger RNA(s) related to secretory proteins in the mouse epididymis

Androgen-dependent messenger RNA(s) related to secretory proteins in the mouse epididymis

The lipocalin sperm coating lizard epididymal secretory protein family: mRNA structural analysis and sequential expression during the annual cycle of the lizard, Lacerta vivipara

Regulation of the epididymal glutathione peroxidase-like protein in the mouse: Dependence upon androgens and testicular factors

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