Characterization Activities to Evaluate Chlorinated Solvent Discharges to Tims Branch from the A/M Area of the Savannah River Site

Jackson, D. R.

doi:10.2172/775074

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…This waste was composed primarily of U and Ni with lesser amounts of other metals. Additionally, an ∼8 km 2 subsurface plume of chlorinated organic solvents, primarily trichloroethylene (TCE) and tetrachloroethylene, originating from this processing facility (7) has begun to contaminate riparian sediments along the Tims Branch corridor (8). Previous studies have examined the chemical and biological availability of Ni and U at this site.…”

Section: Introductionmentioning

confidence: 99%

Reduction of Nickel and Uranium Toxicity and Enhanced Trichloroethylene Degradation to Burkholderia vietnamiensis PR1₃₀₁ with Hydroxyapatite Amendment

Nostrand

Khijniak

Neely

et al. 2007

Environ. Sci. Technol.

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The use of hydroxyapatite (HA) to sequester metals at mixed waste sites may reduce metal toxicity and facilitate microbial degradation of cocontaminant organics. The constitutive trichloroethylene (TCE) degrader, Burkholderia vietnamiensis PR1301, grew at 34.1 and 1.7 mM Ni at pH 5 and 7, respectively, with 0.01 g mL(-1) HA compared to 17 and 0.85 mM Ni without HA. PR1 grew at 4.2 mM U at pH 5 and 7 with 0.01 g mL(-1) HA compared to 1.1 mM U without HA. A similar decrease in the toxicity of Ni and U in combination was observed with HA. The ability of PR1 to degrade TCE at 0.85, 1.7, and 3.4 mM Ni and at 0.42 and 1.1 mM U was examined. The presence of TCE resulted in a decreased tolerance of PR1 to Ni and U; however, HA facilitated TCE degradation in the presence of Ni and U, effectively doubling the metal concentrations at which TCE degradation proceeded. These studies suggest that metal sequestration via HA amendments may offer a feasible approach to reducing metal toxicity to microorganisms at mixed waste sites, thereby enhancing the degradation of cocontaminant organics.

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Section: Introductionmentioning

confidence: 99%

Reduction of Nickel and Uranium Toxicity and Enhanced Trichloroethylene Degradation to Burkholderia vietnamiensis PR1₃₀₁ with Hydroxyapatite Amendment

Nostrand

Khijniak

Neely

et al. 2007

Environ. Sci. Technol.

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“…Various characterization studies and investigations have been conducted since soil and groundwater contamination was first identified in the A/M-Area in 1981 (Marine and Bledsoe, 1984;Pickett et al, 1987;Looney et al, 1992;Jackson et al, 1996;Jackson et al, 1997;Jackson et al, 1999;Jerome et al, 1999;Jackson et al, 2000;Vangelas, 2000). Remediation strategies have been developed and applied to meet the needs and remediation goals of the various parts of the DNAPL plume in the A/M-Area.…”

Section: Introductionmentioning

confidence: 99%

“…Soil vapor extraction and barometric pumping systems have been installed to help remove solvent in the vapor phase from the vadose zone. In addition to these programs, other remediation technologies, such as phytoremediation, Dynamic Underground Stripping, six phase heating, radiofrequency heating, and in-situ oxidation using Fenton's reagent have been demonstrated or implemented as pilot treatment systems to target particular parts of the plume (Jerome et al, 1997;Jackson et al, 2000;Vangelas, 2000;Jackson and Looney, 2001). …”

Section: Introductionmentioning

confidence: 99%

DOE-EMSP Final Report: Characterization of Changes in Colloid and DNAPL Affecting Surface Chemistry and Remediation

Powers¹,

Grimberg²,

Denham³

2007

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Isolation and Characterization of Four Gram-Positive Nickel-Tolerant Microorganisms from Contaminated Sediments

et al. 2007

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Microbial communities from riparian sediments contaminated with high levels of Ni and U were examined for metal-tolerant microorganisms. Isolation of four aerobic Ni-tolerant, Gram-positive heterotrophic bacteria indicated selection pressure from Ni. These isolates were identified as Arthrobacter oxydans NR-1, Streptomyces galbus NR-2, Streptomyces aureofaciens NR-3, and Kitasatospora cystarginea NR-4 based on partial 16S rDNA sequences. A functional gene microarray containing gene probes for functions associated with biogeochemical cycling, metal homeostasis, and organic contaminant degradation showed little overlap among the four isolates. Fifteen of the genes were detected in all four isolates with only two of these related to metal resistance, specifically to tellurium. Each of the four isolates also displayed resistance to at least one of six antibiotics tested, with resistance to kanamycin, gentamycin, and ciprofloxacin observed in at least two of the isolates. Further characterization of S. aureofaciens NR-3 and K. cystarginea NR-4 demonstrated that both isolates expressed Ni tolerance constitutively. In addition, both were able to grow in higher concentrations of Ni at pH 6 as compared with pH 7 (42.6 and 8.5 mM Ni at pH 6 and 7, respectively). Tolerance to Cd, Co, and Zn was also examined in these two isolates; a similar pH-dependent metal tolerance was observed when grown with Co and Zn. Neither isolate was tolerant to Cd. These findings suggest that Ni is exerting a selection pressure at this site for metal-resistant actinomycetes.

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Characterization Activities to Evaluate Chlorinated Solvent Discharges to Tims Branch from the A/M Area of the Savannah River Site

Cited by 4 publications

References 19 publications

Reduction of Nickel and Uranium Toxicity and Enhanced Trichloroethylene Degradation to Burkholderia vietnamiensis PR1₃₀₁ with Hydroxyapatite Amendment

Reduction of Nickel and Uranium Toxicity and Enhanced Trichloroethylene Degradation to Burkholderia vietnamiensis PR1₃₀₁ with Hydroxyapatite Amendment

DOE-EMSP Final Report: Characterization of Changes in Colloid and DNAPL Affecting Surface Chemistry and Remediation

Isolation and Characterization of Four Gram-Positive Nickel-Tolerant Microorganisms from Contaminated Sediments

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Characterization Activities to Evaluate Chlorinated Solvent Discharges to Tims Branch from the A/M Area of the Savannah River Site

Cited by 4 publications

References 19 publications

Reduction of Nickel and Uranium Toxicity and Enhanced Trichloroethylene Degradation to Burkholderia vietnamiensis PR1301 with Hydroxyapatite Amendment

Reduction of Nickel and Uranium Toxicity and Enhanced Trichloroethylene Degradation to Burkholderia vietnamiensis PR1301 with Hydroxyapatite Amendment

DOE-EMSP Final Report: Characterization of Changes in Colloid and DNAPL Affecting Surface Chemistry and Remediation

Isolation and Characterization of Four Gram-Positive Nickel-Tolerant Microorganisms from Contaminated Sediments

Contact Info

Product

Resources

About

Reduction of Nickel and Uranium Toxicity and Enhanced Trichloroethylene Degradation to Burkholderia vietnamiensis PR1₃₀₁ with Hydroxyapatite Amendment

Reduction of Nickel and Uranium Toxicity and Enhanced Trichloroethylene Degradation to Burkholderia vietnamiensis PR1₃₀₁ with Hydroxyapatite Amendment