Characteristics of Vibrio vulnificus isolated from diseased tilapia.

Sakata, Taizo; Hattori, Muneaki

doi:10.3147/jsfp.23.33

Cited by 26 publications

(18 citation statements)

References 12 publications

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“…water, eels) and clinical isolates (Tison et al 1982, Tsai et al 1990). Biotype 1 has been isolated from seawater (Veenstra et al 1993a,b, Hor et al 1995, Hsueh et al 2004), eels and oysters (Coleman et al 1996) but has thus far been considered non-pathogenic for eels , Coleman et al 1996, although it can cause disease in tilapia and carp (Sakata & Hattori 1988). Biotype 2 has mainly been found in diseased eels (Tsai et al 1990, Biosca et al 1991.…”

Section: Introductionmentioning

confidence: 99%

Vibrio vulnificus outbreaks in Dutch eel farms since 1996: strain diversity and impact

Haenen

Zanten²,

Jansen³

et al. 2014

Dis. Aquat. Org.

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Vibrio vulnificus is a potentially zoonotic bacterial pathogen of fish, which can infect humans (causing necrotic fasciitis). We analysed 24 V. vulnificus isolates (from 23 severe eel disease outbreaks in 8 Dutch eel farms during 1996 to 2009, and 1 clinical strain from an eel farmer) for genetic correlation and zoonotic potential. Strains were typed using biotyping and molecular typing by high-throughput multilocus sequence typing (hiMLST) and REP-PCR (Diversilab ® ). We identified 19 strains of biotype 1 and 5 of biotype 2 (4 from eels, 1 from the eel farmer), that were subdivided into 8 MLST types (ST) according to the international standard method. This is the first report of V. vulnificus biotype 1 outbreaks in Dutch eel farms. Seven of the 8 STs, of unknown zoonotic potential, were newly identified and were deposited in the MLST database. The REP-PCR and the MLST were highly concordant, indicating that the REP-PCR is a useful alternative for MLST. The strains isolated from the farmer and his eels were ST 112, a known potential zoonotic strain. Antimicrobial resistance to cefoxitin was found in most of the V. vulnificus strains, and an increasing resistance to quinolones, trimethoprim + sulphonamide and tetracycline was found over time in strain ST 140. Virulence testing of isolates from diseased eels is recommended, and medical practitioners should be informed about the potential risk of zoonotic infections by V. vulnificus from eels for the prevention of infection especially among high-risk individuals. Additional use of molecular typing methods such as hiMLST and Diversilab ® is recommended for epidemiological purposes during V. vulnificus outbreaks.

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Section: Introductionmentioning

confidence: 99%

Vibrio vulnificus outbreaks in Dutch eel farms since 1996: strain diversity and impact

Haenen

Zanten²,

Jansen³

et al. 2014

Dis. Aquat. Org.

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“…The common bacterial diseases seen in O. mossambicus are caused by Streptococcus sp., Pseudomonas fluorescen s (Miyazaki et al , 1984), Aeromonas hydrophila (Leung et al , 1994), Edwardsiella sp. (Kaige et al , 1986), Vibrio vulnificus (Sakata & Hattori, 1988) and a rickettsia‐like microorganism (Chern & Chao, 1994).…”

Section: Introductionmentioning

confidence: 99%

Dietary medicinal plant extracts improve growth, immune activity and survival of tilapia Oreochromis mossambicus

Immanuel¹,

Uma²,

Iyapparaj³

et al. 2009

Journal of Fish Biology

141

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The effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (Bermuda grass Cynodon dactylon, H(1), beal Aegle marmelos, H(2), winter cherry Withania somnifera, H(3) and ginger Zingiber officinale, H(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia Oreochromis mossambicus were assessed. In addition, the antimicrobial properties of the extract were assessed against Vibrio alginolyticus, Vibrioparahaemolyticus, Vibrio mimicus, Vibrio campbelli, Vibrio vulnificus, Vibrio harveyi and Photobacterium damselae. Oreochromis mossambicus were fed 5% of their body mass per day for 45 days, and those fed the experimental diets showed a greater increase in mass (111-139%) over the 45 days compared to those that received the control diet (98%). The specific growth rate of O. mossambicus fed the four diets was also significantly greater (1.66-1.93%) than control (1.52%) diet-fed fish. The blood plasma chemistry analysis revealed that protein, albumin, globulin, cholesterol, glucose and triglyceride levels of experimental fish were significantly higher than that of control fish. Packed cell volume of the blood samples of experimental diet-fed fish was also significantly higher (34.16-37.95%) than control fish (33.0%). Leucocrit value, phagocytic index and lysozyme activity were enhanced in fish fed the plant extract-supplemented diets. The acetone extract of the plants inhibited growth of Vibrio spp. and P. damselae with extracts from W. somnifera showing maximum growth inhibition. A challenge test with V. vulnificus showed 100% mortality in O. mossambicus fed the control diet by day 15, whereas the fish fed the experimental diets registered only 63-80% mortality at the end of challenge experiment (30 days). The cumulative mortality index for the control group was 12,000, which was equated to 1.0% mortality, and accordingly, the lowest mortality of 0.35% was registered in H(4)-diet-fed group.

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“…The Nile tilapia Oreochromis niloticus is a fish of both fresh and salt water, and the main pathogenic Vibrio serovars involved in salt water infections are V. anguillarum, V. parahaemolyticus and V. vulnificus. While in freshwater cultures V. mimicus and V. cholerae are the main pathogenic serovars involved (Plumb, 1999;Sakata and Hattori, 1988). Here in, isolation of V. harveyi from fresh water fishes and cultivate farms in Egypt warrants more investigations to clarify its public health impact.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Bacteriological and Histopathological Studies on Vibrio Species Isolated from Naturally Infected Freshwater Fish in Delta Region, Egypt

A¹,

AbdEl-Gaber²,

Tarabees³

et al. 2017

Adv. Anim. Vet. Sci.

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Characteristics of Vibrio vulnificus isolated from diseased tilapia.

Cited by 26 publications

References 12 publications

Vibrio vulnificus outbreaks in Dutch eel farms since 1996: strain diversity and impact

Vibrio vulnificus outbreaks in Dutch eel farms since 1996: strain diversity and impact

Dietary medicinal plant extracts improve growth, immune activity and survival of tilapia Oreochromis mossambicus

Bacteriological and Histopathological Studies on Vibrio Species Isolated from Naturally Infected Freshwater Fish in Delta Region, Egypt

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