Characteristics of Vascular Wall Cells Subjected to Dynamic Cyclic Strain and Fluid Shear Conditionsin Vitro

Benbrahim, Aziz; L’Italien, Gilbert; Kwolek, Christopher J.; Petersen, Michael J.; Milinazzo, B.; Gertler, Jonathan P.; Abbott, William M.; Orkin, Roslyn W.

doi:10.1006/jsre.1996.0353

Cited by 17 publications

(14 citation statements)

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“…23,24,29,48,58 Although a number of models have been developed to study vascular biomechanical forces in vitro, relatively few systems replicate the tubular nature of the artery. 5,33,41,43,60,61 Additionally, there are only a limited number of systems capable of delivering more than one biomechanical force simultaneously. Nonetheless, a number of systems have been developed to examine the effects of either shear stress or cyclic stretch on the gene expression of endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Human Endothelial Cells Post Stent Deployment in a Cardiovascular Simulator In Vitro

et al. 2009

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Percutaneous stent implantation has revolutionized the clinical treatment of occluded arteries. Nevertheless, there is still a large unmet need to prevent re-occlusion after implantation. Consequently, a niche exists for a cost-effective pre-clinical method of evaluating novel interventional devices in human models. Therefore, the development of a coronary model artery offers tremendous potential for the treatment of endothelial cell dysfunction and restenosis. As a first step, we employ tissue-engineering principles to examine the effect of stent deployment upon endothelial cells in a tubular in vitro system capable of replicating the coronary artery biomechanical environment. In particular, the cellular and molecular changes pertaining to inflammation, proliferation, and death were assessed after stent deployment. Real-time quantitative PCR demonstrated increased expression of genes encoding for E-Selectin, ICAM-1, and VCAM-1; markers associated with an inflammatory response in vivo. Further, an increase in the pro-apoptotic protein Bax was paralleled with a decrease in the anti-apoptotic protein Bcl-2; however, apoptotic morphology was not observed. Interestingly, transcription of c-fos increased, whereas Ki67 levels fell over the same period. One hypothesis is that these results are in response to the altered local hemodynamic environment induced by stent deployment. Most significantly, this study highlights the potential of a biomimetic hemodynamic bioreactor combined with a gene expression analysis to evaluate, with greater specificity, the performance and interaction of stents with the endothelial layer in a controlled environment.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Human Endothelial Cells Post Stent Deployment in a Cardiovascular Simulator In Vitro

et al. 2009

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“…Transactions of the ASME Table 3 A selection of studies applying both shear stress and strain to cells may be observed with a microscope while still in the mock artery [149,151,164,165]. The morphological characteristics of interest include cell alignment [153,154,157,159,161,164,166], cell elongation [154,159,161], and alignment of cytoskeletal actin filaments [130,149].…”

Section: Neonatal Rat Pulmonary Fibroblastsmentioning

confidence: 99%

Device-Based In Vitro Techniques for Mechanical Stimulation of Vascular Cells: A Review

Davis

Zambrano

Anumolu

et al. 2015

Journal of Biomechanical Engineering

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The most common cause of death in the developed world is cardiovascular disease. For decades, this has provided a powerful motivation to study the effects of mechanical forces on vascular cells in a controlled setting, since these cells have been implicated in the development of disease. Early efforts in the 1970 s included the first use of a parallel-plate flow system to apply shear stress to endothelial cells (ECs) and the development of uniaxial substrate stretching techniques (Krueger et al., 1971, "An in Vitro Study of Flow Response by Cells," J. Biomech., 4(1), pp. 31-36 and Meikle et al., 1979, "Rabbit Cranial Sutures in Vitro: A New Experimental Model for Studying the Response of Fibrous Joints to Mechanical Stress," Calcif. Tissue Int., 28(2), pp. 13-144). Since then, a multitude of in vitro devices have been designed and developed for mechanical stimulation of vascular cells and tissues in an effort to better understand their response to in vivo physiologic mechanical conditions. This article reviews the functional attributes of mechanical bioreactors developed in the 21st century, including their major advantages and disadvantages. Each of these systems has been categorized in terms of their primary loading modality: fluid shear stress (FSS), substrate distention, combined distention and fluid shear, or other applied forces. The goal of this article is to provide researchers with a survey of useful methodologies that can be adapted to studies in this area, and to clarify future possibilities for improved research methods.

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“…The aim of this study is to design and validate a bioreactor that is capable of applying both pulsatile WSS and pulsatile THS of physiological proportions and that will allow much greater flexibility in the range of combinations of WSS and THS that can be applied, in comparison to tubular devices previously used. 33,34 …”

Section: Introductionmentioning

confidence: 98%

“…A number of studies combine both WSS and THS; Benbrahim et al 33 and Peng et al 34 have evaluated the in vitro response of human umbilical vein endothelial cells ͑HUVECs͒ to a combination of both arterial strain and fluid shear in a tubular model. Results show that pulsatile stretch and flow produce cellular alignment and actin cytoskeleton rearrangement as observed in stretch alone models 35 and WSS morphological studies.…”

Section: Introductionmentioning

confidence: 99%

Development of a novel bioreactor to apply shear stress and tensile strain simultaneously to cell monolayers

Breen

McHugh

McCormack

et al. 2006

Review of Scientific Instruments

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To date many bioreactor experiments have investigated the cellular response to isolated in vitro forces. However, in vivo, wall shear stress ͑WSS͒ and tensile hoop strain ͑THS͒ coexist. This article describes the techniques used to build and validate a novel vascular tissue bioreactor, which is capable of applying simultaneous wall shear stress and tensile stretch to multiple cellular substrates. The bioreactor design presented here combines a cone and plate rheometer with flexible substrates. Using such a combination, the bioreactor is capable of applying a large range of pulsatile wall shear stress ͑−30 to + 30 dyn/ cm 2 ͒ and tensile hoop strain ͑0%-12%͒. The WSS and THS applied to the cellular substrates were validated and calibrated. In particular, curves were produced that related the desired WSS to the bioreactor control parameters. The bioreactor was shown to be biocompatible and noncytotoxic and suitable for cellular mechanical loading studies in physiological condition, i.e., under simultaneous WSS and THS conditions.

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Characteristics of Vascular Wall Cells Subjected to Dynamic Cyclic Strain and Fluid Shear Conditionsin Vitro

Cited by 17 publications

References 0 publications

Evaluation of Human Endothelial Cells Post Stent Deployment in a Cardiovascular Simulator In Vitro

Evaluation of Human Endothelial Cells Post Stent Deployment in a Cardiovascular Simulator In Vitro

Device-Based In Vitro Techniques for Mechanical Stimulation of Vascular Cells: A Review

Development of a novel bioreactor to apply shear stress and tensile strain simultaneously to cell monolayers

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