Characteristics of the KSN Inbred Mice: A Strain of Self-Perpetuating Nude Mice

Sudo, Katsuko; Suzuku, Kiyoshi; Matsumoto, Kiyoichi; Furuya, Tsuyoshi; Kurokawa, Yuji

doi:10.1159/000417143

Cited by 5 publications

(4 citation statements)

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“…Seven-week old female KSN athymic nude mice (24) were supplied by Japan SLC (Hamamatsu, Japan). They were maintained under specific-pathogen-free conditions.…”

Section: Methodsmentioning

confidence: 99%

Antitumor activity of mannan-binding proteinin vivoas revealed by a virus expression system: Mannan-binding proteindependent cell-mediated cytotoxicity

Uemura

Oka

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

117

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Mannan-binding protein (MBP), a Ca 2؉ -dependent mammalian lectin specific for mannose and Nacetylglucosamine, is an important serum component associated with innate immunity. MBP activates complement and functions as a direct opsonin on binding to mannooligosaccharide-bearing pathogens. We have found that MBP recognizes and binds specifically to oligosaccharide ligands expressed on the surfaces of a human colorectal carcinoma. Interestingly, the recombinant vaccinia virus carrying human MBP gene was demonstrated to possess a potent growthinhibiting activity against human colorectal carcinoma cells transplanted in KSN nude mice when administered by intratumoral or subcutaneous injection. Moreover, a significant prolongation of life span of tumor-bearing mice resulted from the treatment. This effect appears to be a consequence of local production of MBP. Unexpectedly, the mutant MBP, which had essentially no complement-activating activity, was nearly as active as wild-type MBP. These results indicated that MBP has a previously undescribed cytotoxic activity, which we propose to term MBP-dependent cell-mediated cytotoxicity(MDCC). In addition, this study provides a model for the development of an effective and specific host defense factor for cancer gene therapy.

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“…Seven-week old female KSN athymic nude mice (24) were supplied by Japan SLC (Hamamatsu, Japan). They were maintained under specific-pathogen-free conditions.…”

Section: Methodsmentioning

confidence: 99%

Antitumor activity of mannan-binding proteinin vivoas revealed by a virus expression system: Mannan-binding proteindependent cell-mediated cytotoxicity

Uemura

Oka

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

117

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“…C57BL/6 (B6) mice, B6.Ly5.1 mice, DDD mice and KSN nu/ nu mice were used. KSN nu/nu embryos were obtained by mating KSN nu/nu females, which are highly reproductive (37), with nu/nu males. Mice were mated at night and the females were examined for vaginal plugs the following morning.…”

Section: Micementioning

confidence: 99%

Two distinct steps of immigration of hematopoietic progenitors into the early thymus anlage

Itoi¹,

Kawamoto²,

Katsura³

et al. 2001

International Immunology

123

101

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Thymic epithelial cells, which create a three-dimensionally organized meshwork structure peculiar to the thymus, develop from simple epithelia of the third pharyngeal pouch and cleft during organogenesis. We comparatively investigated the thymus anlages of normal and nude mice by immunohistochemical analysis with regard to epithelial organization and distribution of hematopoietic progenitor cells at early stages of organogenesis. Our results show that development of the mouse thymus anlage at early stages can be subdivided into at least two stages by the differences in epithelial organization, i.e. stratified epithelial stage on embryonic day (Ed) 11 and clustered epithelial stage on Ed12. At the former stage, hematopoietic progenitor cells are accumulated in the mesenchymal layer of the thymus anlage, and at the latter stage progenitor cells enter the epithelial cluster and proliferate. In nude mice, hematopoietic progenitor cells are found in the mesenchymal layer on Ed11.5, but they are not observed among epithelial cells on Ed12, even though epithelial cells form a cluster structure. The present results suggest that aberrant development of the nude mouse thymus anlage occurs at the clustered epithelial stage and that epithelial cells of the nude anlage lack the ability to induce the entrance of hematopoietic progenitor cells into the epithelial cluster.

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“…Male KSN (nu/nu) nude mice [12,13], 9-12 weeksold, purchased from Japan SLC, Inc. (Shizuoka, Japan), were used in the present studies. They were housed in plastic cages with hard wood chips in an air-conditioned room at 23 ± 2°C, with a 12-hr-12-hr light-dark cycle and given food (Oriental MF, Oriental Yeast Co., Ltd., Tokyo, Japan) and water and ad libitum.…”

Section: Animalsmentioning

confidence: 99%

Characteristics of nonneoplastic human prostate tissue transplanted into nude mice

et al. 1998

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Characteristics of the KSN Inbred Mice: A Strain of Self-Perpetuating Nude Mice

Cited by 5 publications

References 0 publications

Antitumor activity of mannan-binding proteinin vivoas revealed by a virus expression system: Mannan-binding proteindependent cell-mediated cytotoxicity

Antitumor activity of mannan-binding proteinin vivoas revealed by a virus expression system: Mannan-binding proteindependent cell-mediated cytotoxicity

Two distinct steps of immigration of hematopoietic progenitors into the early thymus anlage

Characteristics of nonneoplastic human prostate tissue transplanted into nude mice

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