Characteristics of the Induction of Two Double-Stranded RNA-Dependent Enzymes by Interferon in Parental and Variant L Cells

Abstract

The interferon (IFN)-dependent induction of two double-stranded RNA-dependent enzymes was examined in L cells and an L-cell variant (WDIFN) which is highly resistant to the inhibitory effects of IFN on cellular multiplication. IFN, in a concentration-dependent manner, inhibited the multiplication of parental L cells and induced increased levels of the double-stranded RNA-dependent enzymes in parental L cells. Although WDIFN cells were resistant to the antiproliferative effects of IFN, the cells responded to IFN by increasing their levels of the double-stranded RNA-dependent enzymes. However, the level of activity of each enzyme was lower in the WDIFN line than the parental line when both lines were treated with similar concentrations of IFN. The reduced response of the WDIFN line was not the result of the line being a heterogeneous population of cells nor of IFN being more unstable in the presence of WDIFN cells. In addition there was no evidence that WDIFN cells produced a mitogenic factor that could overcome the antiproliferative effects of IFN, nor that sodium butyrate could increase the sensitivity of WDIFN cells to the antiproliferative effects of IFN.