Characteristics of the antiulcerogenic activity of cryopreserved placenta extract in acute and chronic lesions of the stomach

Hladkykh, Fedir; Кошурба, Ілля; Chyzh, Mykola

doi:10.34287/mmt.1(56).2023.10

Cited by 11 publications

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References 11 publications

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“…До диспергованої тканини додають 0,9 %-вого розчину NaCl (1:2), витримують добу за температури 4 С і центрифугують 15-20 хв за 4000 об/хв. Одержаний надосад фільтрують через фільтри діаметром 0,22 мкм, фасують в ампули прозорого скла по 1,8 мл і зберігають у рідкому азоті [21,27,35].…”

Section: матеріали і методиunclassified

The Characteristic of the Gastric Secretion After Gastric Cryodenervation and Cryopreserved Placenta Extract Administration

Кошурба

Hladkykh

Chyzh

et al. 2023

Innov Biosyst Bioeng

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Background. Peptic ulcer is a prevalent gastrointestinal tract disease worldwide. One of the treatment methods for the indicated pathology is trunk gastric cryodenervation (TGC), the feature of which is the preservation of the regenerative potential of n. vagus. Cryopreserved placenta extract (CPE) caught our attention among modern means capable of stimulating regeneration processes. Objective. We are aimed to characterize the state of gastric secretion after TGC and the application of СPE. Methods. TGC was performed by cryoinfusion on the front and back trunks of the abdominal part of n. vagus. Gastric secretion was studied using the H.A. Shay method. Results. On the 30th day after TGC, a statistically significant (p < 0.001) decrease in the total acidity of gastric juice by 33.7% and a decrease (p < 0.01) in the level of free acidity were observed compared to intact animals. Administration of СPE after TGC resulted in a statistically significant (p < 0.001) decrease in total acidity by 24.2 % and a statistically significant (p < 0.001) decrease in free acidity by 48.7% compared to the parameters of intact rats. This led to a statistically significant (p < 0.001) decrease in the "free/bound acidity" ratio compared to the indicator of intact animals (1.4 ± 0.03 RU) which was higher than the similar ratio observed after esomeprazole usage or TGC. Conclusions. Administration of СPE contributed to the partial regeneration of n. vagus, as indicated by an increase in the level of total acidity to 104.1 ± 4.7 RU and a statistically significant (p < 0.001) decrease in the "free/bound acidity" ratio compared to the indicator of intact animals.

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Section: матеріали і методиunclassified

The Characteristic of the Gastric Secretion After Gastric Cryodenervation and Cryopreserved Placenta Extract Administration

Кошурба

Hladkykh

Chyzh

et al. 2023

Innov Biosyst Bioeng

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Gender Differences in the Effect of Antiulcer Drugs and Placenta Cryoextract on the Intensity of Lipid Peroxidation and the Activity of the Antioxidant System in Experimental Hepatitis With Ethanol-Induced Cirrhosis

Koshurba,

Hladkykh,

Chyzh

et al. 2023

Clin. and prev. med.

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Introduction. It is well recognized that drug metabolism products in the liver can induce oxidative stress and mitochondrial dysfunction, leading to the development of hepatocellular injury. As a potential agent capable of counteracting the hepatotoxic effects of drugs, we focused our attention on a domestic biotechnological preparation – cryopreserved placental extract (CPE). The aim. To characterize gender differences in the effect of esomeprazole, clarithromycin, metronidazole (E/C/M), and CPE on the intensity of lipid peroxidation and the activity of the antioxidant system in tetrachloromethane (CCl4) hepatitis with a background of ethanol-induced cirrhosis (ETCM). Materials and methods. The study was conducted with varying levels of sex hormones on 112 male and female rats. Chronic ETCM was induced by administering a 50.0% oil solution of CCl4 at a dose of 8 ml/kg body weight of the animals twice a week, in combination with a 5.0% ethanol solution for drinking over a period of 45 days. The content of TBA-RP in liver homogenates was determined spectrophotometrically by the method described by Asakawa T. et al. Catalase activity in liver homogenates was determined spectrophotometrically according to the method of Korolyuk M.A. and co-authors. Results. The most pronounced increase in lipid peroxidation processes was observed in females with chronic ETCM-induced liver damage and administration of antiulcer drugs following ovariectomy, resulting in a TBA-RS content of 36.1±2.79 μmol/kg of tissue. Administration of E/C/M in animals with chronic liver damage led to a suppression of the antioxidant system, as evidenced by a decrease in catalase activity in liver tissues. Conclusion. The combined use of anti-ulcer drugs and CPE on the background of chronic ETCM mitigated the activation of lipid peroxidation processes, which was indicated by a statistically significant (p < 0.001) 2.7-fold lower content of TBA-RP in liver homogenates. Additionally, it was established that the administration of CPE was accompanied by a statistically significant increase in catalase activity in females, more prominently than in males. In females without changes in hormonal status, the introduction of CPE resulted in a growth (p < 0.001) of catalase activity by 75.0%, with the most significant increase observed in females after ovariectomy – catalase activity statistically significantly (p < 0.001) increased by 2.6 times compared to the indicators of females not administered with CPE. The administration of CPE in female rats without altering hormonal status was accompanied by a twofold (p < 0.01) increase in the antioxidant-prooxidant index compared to male rats, indicating more pronounced antioxidant properties of CPE in female rats.

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Modern approaches to the treatment of peptic ulcer disease and prospects for the use of biological therapy

Кошурба

Hladkykh

Chyzh³

2023

Modern Medical Technology

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Digestive diseases are the sixth leading cause of death in the world and accounted for more than 2.5 million deaths in 2019. In Ukraine, mortality from diseases of the digestive system is recorded as one of the highest in the world, and the leading place in the general structure of diseases of the digestive system is occupied by peptic ulcer disease (РUD). The aim of the study. Summarize current information on the etiology and the pathogenesis of РUD and characterize modern approaches to the treatment of patients with РUD and the prospects of biological therapy. Materials and methods. Publications were selected based on the PubMed, Clinical Key Elsevier, Cochrane Library, eBook Business Collection, and Google Scholar databases, which covered information on the etiology, pathogenesis, and approaches to the treatment of РUD. Results. Standard first-line anti-Helicobacter therapy consists of a proton pump inhibitor and two antibiotics, such as clarithromycin and amoxicillin or metronidazole. A promising direction in the treatment of РUD is the use of biological therapy. According to literature sources, the prophylactic use of cryoextract of the placenta in indomethacin gastric lesions has an anti-ulcer effect at the level of 69.1 % and 92.1 % in diclofenac sodium gastric lesions. In addition, the specified cryoextract is capable of leveling the gastrotoxic effect of acetylsalicylic acid, ibuprofen, stress factor and chemical ulcerogens. Conclusions. Today, there is a great need for cell therapy that could be put into practice in clinically relevant volumes. The most promising directions of biological therapy in gastroenterology are considered to be the use of mesenchymal stem cells and agents obtained from the fetoplacental complex.

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Characteristics of the antiulcerogenic activity of cryopreserved placenta extract in acute and chronic lesions of the stomach

Cited by 11 publications

References 11 publications

The Characteristic of the Gastric Secretion After Gastric Cryodenervation and Cryopreserved Placenta Extract Administration

The Characteristic of the Gastric Secretion After Gastric Cryodenervation and Cryopreserved Placenta Extract Administration

Gender Differences in the Effect of Antiulcer Drugs and Placenta Cryoextract on the Intensity of Lipid Peroxidation and the Activity of the Antioxidant System in Experimental Hepatitis With Ethanol-Induced Cirrhosis

Modern approaches to the treatment of peptic ulcer disease and prospects for the use of biological therapy

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