Characteristics of Stenotrophomonas maltophilia isolates from cystic fibrosis patients in Russia

Bocharova, Yu.A.; Savinova, Tatiana; Lyamin, A. V.; Kondratenko, O.; Поликарпова, С. В.; Жилина, С. В.; Fedorova, Natalia; Semykin, S. Yu.; Chaplin, Andrei V.; Korostin, Dmitriy; Mayanskiy, Nikolay; Chebotar, I. V.

doi:10.51620/0869-2084-2022-67-5-315-320

Cited by 2 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S. maltophilia is an inherently multi-drug resistant organism, and persistent infection has been associated with increased risk of pulmonary exacerbation, lung transplantation and death [67]. Here, we observed persistent colonisation with S. maltophilia ST4 in one patient, which has been shown to be a common ST among CF patients [68]. H. influenzae is considered a commensal of the nasopharynx, and, while it is a common coloniser of the CF airway in childhood, its role in CF disease is controversial as there is limited evidence of the impact of chronic H. influenzae colonisation on clinical course.…”

Section: Discussionmentioning

confidence: 59%

Insights into the Adolescent Cystic Fibrosis Airway Microbiome Using Shotgun Metagenomics

McDermott,

Walsh,

Crispie

et al. 2024

IJMS

View full text Add to dashboard Cite

Cystic fibrosis (CF) is an inherited genetic disorder which manifests primarily in airway disease. Recent advances in molecular technologies have unearthed the diverse polymicrobial nature of the CF airway. Numerous studies have characterised the genus-level composition of this airway community using targeted 16S rDNA sequencing. Here, we employed whole-genome shotgun metagenomics to provide a more comprehensive understanding of the early CF airway microbiome. We collected 48 sputum samples from 11 adolescents and children with CF over a 12-month period and performed shotgun metagenomics on the Illumina NextSeq platform. We carried out functional and taxonomic analysis of the lung microbiome at the species and strain levels. Correlations between microbial diversity measures and independent demographic and clinical variables were performed. Shotgun metagenomics detected a greater diversity of bacteria than culture-based methods. A large proportion of the top 25 most-dominant species were anaerobes. Samples dominated by Staphylococcus aureus and Prevotella melaninogenica had significantly higher microbiome diversity, while no CF pathogen was associated with reduced microbial diversity. There was a diverse resistome present in all samples in this study, with 57.8% agreement between shotgun metagenomics and culture-based methods for detection of resistance. Pathogenic sequence types (STs) of S. aureus, Pseudomonas aeruginosa, Haemophilus influenzae and Stenotrophomonas maltophilia were observed to persist in young CF patients, while STs of S. aureus were both persistent and shared between patients. This study provides new insight into the temporal changes in strain level composition of the microbiome and the landscape of the resistome in young people with CF. Shotgun metagenomics could provide a very useful one-stop assay for detecting pathogens, emergence of resistance and conversion to persistent colonisation in early CF disease.

show abstract

Section: Discussionmentioning

confidence: 59%

Insights into the Adolescent Cystic Fibrosis Airway Microbiome Using Shotgun Metagenomics

McDermott,

Walsh,

Crispie

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…There is a concern about the rising antimicrobial resistance of SXT and FQs among S. maltophilia strains. Recent data reveals that the rate of SXT resistance ranges from 9.7% to 21.6% ( 13 , 14 , 23 , 24 ). Levofloxacin resistance is also concerning due to the increased use of FQs and changes from 9.7% to 24% in different studies ( 14 , 23 , 24 ).…”

Section: Discussionmentioning

confidence: 99%

“…Recent data reveals that the rate of SXT resistance ranges from 9.7% to 21.6% ( 13 , 14 , 23 , 24 ). Levofloxacin resistance is also concerning due to the increased use of FQs and changes from 9.7% to 24% in different studies ( 14 , 23 , 24 ). In our study, SXT and levofloxacin resistance was not high and seemed lower than many other reports (10.9% and 12.5%, respectively).…”

Section: Discussionmentioning

confidence: 99%

Stenotrophomonas maltophilia Bacteremia: From Diagnosis to Treatment

Yıldız

Habibi

et al. 2022

Infect Dis Clin Microbiol

View full text Add to dashboard Cite

Objective: There are many difficulties in diagnosing and treating Stenotrophomonas maltophilia bacteremia. In this study, we aimed to evaluate “true” and “false-positive bacteremia” and assess mortality risk factors and the impact of different treatment regimens. Materials and Methods: Hospitalized adult patients with S. maltophilia-positive blood cultures were assessed by a two-stage analysis. First, the clinical significance of blood cultures was assessed, and patients were divided into “true” and “false-positive bacteremia” groups. Then, excluding false positives, we analyzed the antimicrobial regimens and the factors associated with 28-day mortality in true bacteremia cases performing univariate and multivariate analyses. Results: The study included 127 out of 138 patients with S. maltophilia bacteremia. True bacteremia was identified in 51.2% and false-positive bacteremia in 48.8% of patients. In the true bacteremia group, hypotension, nosocomial bacteremia, concomitant infections, a source of bacteremia, two positive blood culture sets, and 28-day mortality were more common. The 28-day mortality was 50.7% among true bacteremia cases. In multivariate analysis, age and solid tumor were the independent predictors of 28-day mortality. Early effective antimicrobial therapy and different antimicrobial regimens, including trimethoprim-sulfamethoxazole (SXT), fluoroquinolones (FQs), and tigecycline (TGC), did not have any significant impact on survival. Conclusion: Patients with S. maltophilia bacteremia should first be assessed regarding clinical significance. Clinical findings, the presence of multiple positive blood culture sets and the primary sources of bacteremia are useful parameters while discriminating true from false-positive bacteremia. Patients with advanced age and solid tumors should be followed carefully in terms of mortality. Antimicrobial regimens, including SXT, FQs, or TGC, can be preferred in patients with S. maltophilia bacteremia considering antimicrobial resistance and adverse effects or toxicity. Keywords: Stenotrophomonas maltophilia, true bacteremia, false-positive bacteremia, blood contamination, mortality, treatment

show abstract

Characteristics of Stenotrophomonas maltophilia isolates from cystic fibrosis patients in Russia

Cited by 2 publications

References 20 publications

Insights into the Adolescent Cystic Fibrosis Airway Microbiome Using Shotgun Metagenomics

Insights into the Adolescent Cystic Fibrosis Airway Microbiome Using Shotgun Metagenomics

Stenotrophomonas maltophilia Bacteremia: From Diagnosis to Treatment

Contact Info

Product

Resources

About