Characteristics of slow-miniature endplate currents show a subunit composition

Vautrin, Jean; Kriebel, Mahlon E.

doi:10.1016/0306-4522(91)90201-x

Cited by 34 publications

(54 citation statements)

References 55 publications

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“…This is consistent with the multiple spikes on the differentiated rising phase of the larger and generally slower TTX-and QX-314-resistant synaptic Cl-currents (Fig. 4Bal, Ba2, Bdl and Bd42) (see also Vautrin & Kriebel, 1991 Most importantly, complex rising phases were often similar in both neurones (Fig. 4Bdl and Bd2), suggesting that they were not a characteristic of the postsynaptic detection of the transmitter packet, nor signal attenuation by cable properties of neurites.…”

Section: Discussionsupporting

confidence: 81%

“…Ropert et al (1990) have shown that there are more short intervals between GABAergic miniature Cl-currents in hippocampal slices than expected from random occurrence, suggesting shortterm interactions between spontaneous transmitter releases. Interdependent transmitter releases occurring at intervals shorter than the rise time of the elementary postsynaptic response integrate postsynaptically and produce complex synaptic signals with variable amplitudes and rise times (see Vautrin & Kriebel, 1991;Vautrin et al 1993). Indeed, Edwards et al (1990) have noticed that frequency distributions of hippocampal GABAergic miniature signal amplitudes may show peaks, indicating that they are caused by a variable number of elementary transmitter releases.…”

Section: Discussionmentioning

confidence: 99%

“…GABAA receptor-activated synaptic transient Clcurrents were routinely recorded in 3 day to 3 week cultures (Vautrin & Kriebel, 1991;Vautrin, Schaffner & Barker, 1993 inward regenerative currents resembling action potentials (Fig. 3A a and Ab).…”

Section: General Observationsmentioning

confidence: 98%

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Fast presynaptic GABAA receptor‐mediated Cl‐ conductance in cultured rat hippocampal neurones.

Vautrin

Schaffner

Barker

1994

The Journal of Physiology

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1. Hippocampal neurones cultured from the 18-day-old embryonic rat for 3 days to 3 weeks were recorded with Cl--filled patch pipettes. Spontaneous synaptic currents, which reversed at the equilibrium potential for Cl-ions (Ec1) and were blocked by the GABAA (y-aminobutyric acid) receptor antagonists bicuculline or picrotoxin, were recorded in every culture. At 25°C and -80 mV they decayed with a time constant > 20 ms that invariably increased at positive potentials. After 2 weeks, 50-75% of all neurones were GABA immunoreactive. 2. In pairs-recordings, coincident synaptic currents in both cells were either spontaneous or evoked by stimulation of one cell. In the presence of tetrodotoxin and using pipettes containing lidocaine (lignocaine) N-ethyl bromide, coincident spontaneous Cl-transients still occurred in both neurones far more frequently than expected by chance. 3. Holding the potential of one neurone at a positive value reversed the synaptic transients in that cell and, in half of the cells, increased the frequency of coincident events in both cells. 4. In neurones where depolarization increased the frequency of coinciding events and all regenerative current apparent at the soma was abolished, short depolarizing pulses occasionally evoked all-or-none, pre-and postsynaptic currents with matching transmission failures and identical delays in transmission. 5. The results suggest that the same pulse of GABA simultaneously activates GABAA receptor-coupled Cl-channels on both sides of the same synaptic cleft, producing immediate auto-transmission in the absence of collaterals or interneurones.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Fast presynaptic GABAA receptor‐mediated Cl‐ conductance in cultured rat hippocampal neurones.

Vautrin

Schaffner

Barker

1994

The Journal of Physiology

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“…This is especially true if "clusters" of MEPPs occurred (Fatt and Katz, 1952;Kriebel and Stolper, 1975;Vautrin and Kriebel, 1991) for whatever reason during a brief recording episode. Alternatively, perhaps younger mice exhibit a greater difference in MEPP frequency between the two genotypes.…”

Section: N-and R-type Camentioning

confidence: 99%

Acetylcholine Release at Neuromuscular Junctions of Adult Tottering Mice Is Controlled by N-(Ca_v2.2) and R-Type (Ca_v2.3) but Not L-Type (Ca_v1.2) Ca²⁺Channels

Pardo

Hajela

Atchison

2006

J Pharmacol Exp Ther

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“…There are no presynaptic specializations similar to the active zones of the endplate (Couteaux & Pecot-Dechavassine, 1973) in the Torpedo electric organ, and the synaptic vesicles are almost twice the size of those of the motor nerve terminals. potentials with a slow and sometimes irregular time course (Kriebel & Gross, 1974;Thesleff & Molgo, 1983;Vautrin & Kriebel, 1991). It has been proposed that spontaneous and evoked quanta are built up of a discrete number of subunits but this is still a matter of controversy (see Matteson, Kriebel & Llados, 1981;Erxleben & Kriebel, 1988b).…”

Section: Introductionmentioning

confidence: 99%

Space and time characteristics of transmitter release at the nerve‐electroplaque junction of Torpedo.

Girod¹,

Corrèges²,

Jacquet³

et al. 1993

The Journal of Physiology

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MEPPs. 6. Composite MEPPs with multiple peaks as well as bursts of small MEPPs were often encountered, even during periods of low frequency. They were suggestive of a complete disorganization of quantal events.7. Fast, slow and composite MEPPs were analysed using the computer model. To simulate the entire variety of signals we had to assume that the MEPPs were generated by either synchronized or desynchronized emission of small quantities of transmitter. The typical relationship observed between amplitude and time course in the population of fast MEPPs suggested that the different amounts of transmitter composing a quantum were delivered synchronously close to each other (either at the same spot or at less than 200 nm apart); it is proposed that they acted on overlapping fields of receptors and that their responses summed up in a superadditive manner.8. Computer analysis of the slow-rising MEPPs was of particular interest since their rapid decay phase indicated that the postsynaptic links (cholinesterase and receptors kinetics) were apparently not altered in this subpopulation. More probably their slow and often irregular rate of rise arose from some desynchronization of the release process.9. It is concluded that at the nerve-electroplaque junction evoked transmitter release operates in the form of quanta containing ca 10000 acetylcholine molecules; the quanta activate independent but closely adjacent postsynaptic fields. Each quantum is apparently composed of a preferential number of subunits emitted at the same point, or very close to each other. The subunits are delivered synchronously in the majority of events (fast MEPPs) but subunit desynchronization occasionally occurs (slow-rising and composite MEPPs).

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Characteristics of slow-miniature endplate currents show a subunit composition

Cited by 34 publications

References 55 publications

Fast presynaptic GABAA receptor‐mediated Cl‐ conductance in cultured rat hippocampal neurones.

Fast presynaptic GABAA receptor‐mediated Cl‐ conductance in cultured rat hippocampal neurones.

Acetylcholine Release at Neuromuscular Junctions of Adult Tottering Mice Is Controlled by N-(Ca_v2.2) and R-Type (Ca_v2.3) but Not L-Type (Ca_v1.2) Ca²⁺Channels

Space and time characteristics of transmitter release at the nerve‐electroplaque junction of Torpedo.

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Characteristics of slow-miniature endplate currents show a subunit composition

Cited by 34 publications

References 55 publications

Fast presynaptic GABAA receptor‐mediated Cl‐ conductance in cultured rat hippocampal neurones.

Fast presynaptic GABAA receptor‐mediated Cl‐ conductance in cultured rat hippocampal neurones.

Acetylcholine Release at Neuromuscular Junctions of Adult Tottering Mice Is Controlled by N-(Cav2.2) and R-Type (Cav2.3) but Not L-Type (Cav1.2) Ca2+Channels

Space and time characteristics of transmitter release at the nerve‐electroplaque junction of Torpedo.

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Acetylcholine Release at Neuromuscular Junctions of Adult Tottering Mice Is Controlled by N-(Ca_v2.2) and R-Type (Ca_v2.3) but Not L-Type (Ca_v1.2) Ca²⁺Channels