Characteristics of refold acid urease immobilized covalently by graphene oxide-chitosan composite beads

Liu, Yang; Liu, Xiaofeng; Zhou, Nandi; Tian, Yaping

doi:10.1016/j.jbiosc.2018.06.012

Cited by 14 publications

(7 citation statements)

References 36 publications

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“…Our results are comparable to previous reports on immobilized urease activity. [54][55][56] Similarly, a study of the performance of our immobilized urease across a range of temperatures revealed consistent ureahydrolyzing action across 20-45 °C with a drop-in activity only beyond 50 °C (Fig. 9).…”

Section: Ph and Temperature Effects And Reusability Of Immobilized Ur...supporting

confidence: 52%

“…We next investigated the kinetics of urease nanoconstructs towards SUr and HUr. Based on the Lineweaver-Burk formalism of Michaelis-Menten kinetics eqn (3), 54 the kinetic parameters of the urease-catalyzed hydrolysis of urea for both synthetic and real fresh urine could be determined. 55…”

Section: Kinetics Of Immobilized Urease Towards Sur and Hurmentioning

confidence: 99%

“…The v max value is indicative of the enzyme's theoretical maximal rate. 54,57 The maximum velocity of urease can be estimated from the intercept of the straight line (1/v max ), while the slope provides the ratio of the Michaelis constant to the maximum hydrolysis rate (k m /v max ). The Lineweaver-Burk method results in comparable k m of urease immobilized GA/CS/ MIONPs against SUr (14.86 mM) and HUr (12.36 mM) (Fig.…”

Section: Kinetics Of Immobilized Urease Towards Sur and Hurmentioning

confidence: 99%

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Ammonium release in synthetic and human urine by a urease immobilized nanoconstruct

Diasi,

Singh,

Mahapatra

et al. 2024

RSC Adv.

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Section: Ph and Temperature Effects And Reusability Of Immobilized Ur...supporting

confidence: 52%

Section: Kinetics Of Immobilized Urease Towards Sur and Hurmentioning

confidence: 99%

Section: Kinetics Of Immobilized Urease Towards Sur and Hurmentioning

confidence: 99%

See 1 more Smart Citation

Ammonium release in synthetic and human urine by a urease immobilized nanoconstruct

Diasi,

Singh,

Mahapatra

et al. 2024

RSC Adv.

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“…We observe that certain plasmids were used for specific purposes; a similar observation to that of the specialized strains discussed previously. The pCold(I–III) plasmid set was used for expression at cold temperatures i.e., whereby expression is induced by the cold-shock response [ 83 , 101 , 102 ]; this plasmid was used in conjunction with ArcticExpress (DE3) for suitable expression at temperatures below 13 °C. pACYC-Duet-1, with its two multiple cloning site locations, was utilized for the co-expression of native chaperones proteins from Pyrococcus furiosus as a strategy to promote proper folding of an α-amylase [ 103 ].…”

Section: Current Experimental Design Practicesmentioning

confidence: 99%

Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications

2021

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Recombinant enzyme expression in Escherichia coli is one of the most popular methods to produce bulk concentrations of protein product. However, this method is often limited by the inadvertent formation of inclusion bodies. Our analysis systematically reviews literature from 2010 to 2021 and details the methods and strategies researchers have utilized for expression of difficult to express (DtE), industrially relevant recombinant enzymes in E. coli expression strains. Our review identifies an absence of a coherent strategy with disparate practices being used to promote solubility. We discuss the potential to approach recombinant expression systematically, with the aid of modern bioinformatics, modelling, and ‘omics’ based systems-level analysis techniques to provide a structured, holistic approach. Our analysis also identifies potential gaps in the methods used to report metadata in publications and the impact on the reproducibility and growth of the research in this field.

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“…(1) Urease can degrade the urea content. Urease is often added to hydrolyze the urea during the production of grape wine and Japanese sake ( Yang et al, 2019 ). The urea content in rice wine could be reduced by more than 90% using urease ( Liu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301

Liang

Ren

et al. 2022

Front. Microbiol.

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There is a potential safety risk with ethyl carbamate (EC) in Hongqu Huangjiu production; 90% of the EC in rice wine is produced by the reaction of the urea with the alcohol of Saccharomyces cerevisiae. In our previous experiments, we screened and obtained a S. cerevisiae strain JH301 that offered low urea production. However, the key genes responsible for low urea production of strain JH301 remain unclear. Here, the whole genome sequencing of S. cerevisiae strain JH301 was accomplished via a next-generation high-throughput sequencing and long-read sequencing technology. There are six main pathways related to the urea metabolism of strain JH301 based on KEGG pathway mapping. Three species-specific genes are related to the urea metabolism pathways and were found in comparative genome analysis between strains JH301 and S288c during Hongqu Huangjiu production for the first time. Finally, the ARG80 gene was found to be likely a key gene responsible for low urea production of S. cerevisiae strain JH301, as determined by PCR and qRT-PCR check analyses from DNA and RNA levers. In conclusion, the results are useful for a scientific understanding of the mechanism of low urea production by Saccharomyces cerevisiae during Hongqu Huangjiu fermentation. It also is important to control the urea and EC contents in Hongqu Huangjiu production.

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Characteristics of refold acid urease immobilized covalently by graphene oxide-chitosan composite beads

Cited by 14 publications

References 36 publications

Ammonium release in synthetic and human urine by a urease immobilized nanoconstruct

Ammonium release in synthetic and human urine by a urease immobilized nanoconstruct

Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications

Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301

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