Characteristics of receptor- and transducer-coupled activation of the intracellular signalling in sensory neuron revealed by atomic force microscopy

Khalisov, M. M.; Penniyaynen, V. A.; Esikova, N. A.; Ankudinov, A. B.; Крылов, Б. В.

doi:10.1134/s1063785017010072

Cited by 3 publications

(1 citation statement)

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“…The AFM sample preparation protocol one can find in [20]. Dorsal root ganglia neurons of 10-12-day chicken embryos were cultivated on polylysine in the presence of CA (10 -8 М) or ouabain (10 -10 М).…”

Section: Resultsmentioning

confidence: 99%

Peculiarities of living cell response to the external stimuli revealed via quasistatic mode of atomic force microscopy

Khalisov

Ankudinov

Penniyaynen

et al. 2017

IOP Conf. Ser.: Mater. Sci. Eng.

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IntroductionIn the last decades, atomic force microscopy (AFM) has become one of the common tools in cell biology research. Now AFM is widely used for living animal cell characterization [1,2]. This imaging method allows studying cell morphology and mechanical properties with submicrometer resolution and under physiologically relevant conditions. Various cellular processes are associated with mechanical properties of cells: shape maintenance, migration, differentiation, and division [3]. Several pathologies are known to change the cell mechanical phenotype [4]. Although the living cells are not perfectly elastic objects, in AFM studies their mechanical properties are mostly described by Young's modulus. The measurement of the Young's modulus of a single cell is based on the AFM nanoindentation technique. It implies that the AFM probe compresses the sample with a preset force, and the induced cell deformation is employed to calculate the Young's modulus according to the selected contact mechanics model [3].AFM Force Spectroscopy and Force Volume are the most widespread modes for living cell Young's modulus measurements. These modes are slow in operation and have low data flow rate. Recently developed quasistatic AFM modes, such as PeakForce QNM (Bruker), are much faster; therefore, they appear to be promising for living cell examination. PeakForce QNM and other similar modes force the AFM tip oscillate well below its resonance frequency and press against the sample surface for a short period of time. The manner of operation enables simultaneous topography and mechanical properties data acquisition. Since PeakForce QNM mode was integrated into commercial AFM systems not long ago, the information on its application to living cells is pretty scarce [5][6][7]. We utilized AFM in PeakForce QNM mode to study the mechanical properties of different types of living cells. The aim of this work was to examine and analyze the specifics of AFM investigation of living chicken embryo cardiac fibroblasts, rat erythrocytes, chicken embryo sensory neurons, and mouse microvascular endothelial cells under physiologically relevant conditions.

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Section: Resultsmentioning

confidence: 99%