Characteristics of Propionibacterium and Arachnia isolated from carious dentine.

Hoshino, Eiichi; Horigome, Toshiyuki; Kagawa, Ryoko; Kaketa, Akira; Okuda, Reiichi

doi:10.2330/joralbiosci1965.26.276

Cited by 9 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation was rarely reported in previous studies. As a propionic acid-producing bacterium, Propionibacterium was once isolated from carious dentin and could decompose collagen and cause dentin caries ( Hoshino et al, 1984 ). Another team has recently detected Propionibacterium in the microbial community from the apical area of persistent apical periodontitis ( Zhou & Wang, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Taxonomic and functional alterations in the salivary microbiota of children with and without severe early childhood caries (S-ECC) at the age of 3

Tang

Xü²,

Zhou

et al. 2022

PeerJ

View full text Add to dashboard Cite

Background Primary dental caries is the most prevalent oral disease among preschool children, which can cause severe damage to teeth and even affect the mental well-being of children. Various studies have demonstrated that the oral microbiome plays a pivotal role in the onset and development of dental caries. However, it remains uncertain about the key microbial markers associated with caries, owing to the limited evidence. Methods Fifteen S-ECC children and fifteen healthy controls were selected from three-year-old children in this study. Their clinical data and oral saliva samples were collected. Shotgun sequencing was conducted to investigate the microbial differences and the relevant functions between the two groups. Results We observed no apparent difference in oral microbial community diversity between the two groups. Still, at the genus/species levels, several characteristic genera/species such as Propionibacterium, Propionibacterium acidifaciens, Prevotella denticola, Streptococcus mutans and Actinomyces sp. oral taxon 448/414 increased significantly in S-ECC children, compared with the oral health group. Furthermore, we found that functional pathways involving glycolysis and acid production, such as starch and sucrose metabolism, fructose and mannose metabolism, glycolysis/gluconeogenesis, were prominently up-regulated in the high-caries group. Conclusions Our study showed that dental caries in children were associated with the alterations in the oral microbiota at the composition and functional levels, which may potentially inspire the exploration of microbial diagnosis or therapeutic treatments.

show abstract

Section: Discussionmentioning

confidence: 99%

Taxonomic and functional alterations in the salivary microbiota of children with and without severe early childhood caries (S-ECC) at the age of 3

Tang

Xü²,

Zhou

et al. 2022

PeerJ

View full text Add to dashboard Cite

show abstract

“…Bacterial strains were maintained on Brain Heart Infusion (BHI: Becton Dickinson, Cockeysville, MD, USA)-blood (sheep) agar plates (7,8). P. acnes was cultured in 100 ml of BHI broth (9,10) under aerobic conditions at 378C, and harvested at the logarithmic growth phase (optical density (OD) at 660 nm 0/2.0 Á/2.5) as live organisms. P. anaerobius cells were cultured anaerobically in Trypticase Soy Broth (TSB: Becton Dickinson) (11) in an anaerobic glovebox (AZ-Hard, Hirasawa Works, Tokyo, Japan) containing a nitrogen atmosphere with 10% H 2 and 10% CO 2 , and harvested at the logarithmic growth phase (OD at 660 nm 0/1.0) as live organisms.…”

Section: Methodsmentioning

confidence: 99%

Aerotolerance of Peptostreptococcus anaerobius biofilms

Fukuda

Takenaka

Kota

et al. 2004

Microbial Ecology in Health and Disease

Self Cite

View full text Add to dashboard Cite

The aim of this study was to establish a method for quantitative differential counting of live/dead anaerobic microorganisms. Artificial biofilms of Peptostreptococcus anaerobius 4330-1, one of the strictest anaerobes, were made on filters of 0.4 mm pore size. A commercial Live/ Dead BacLight Bacterial Viability Kit using propidium iodide (PI) and SYTO9 fluorescent staining was found to be suitable for the facultatively anaerobic organism Propionibacterium acnes. However, P. anaerobius seemed to reduce SYTO9 and change fluorescent intensity, which made the kit unsuitable for this anaerobe. As an alternative method suitable for even P. anaerobius, a combination of phasecontrast microscopy and PI staining was adapted. Bacterial numbers could be counted in phase-contrast microscopic fields, and PI-positive organisms were counted as dead organisms in the same field. Only 3.8% of P. anaerobius cells in artificial biofilms were found to be PIpositive after exposure to air for 24 h, while 44% of planktonic P. anaerobius cells were PI-positive after exposure to air for 24 h. Similarly, 38% of P. anaerobius cells were PI-positive when organisms were collected onto filters to make bacterial masses and thereafter exposed to air for 24 h. These results indicate that P. anaerobius is highly aerotolerant in biofilms.

show abstract

Predominant Obligate Anaerobes in Human Carious Dentin

1985

View full text Add to dashboard Cite

With the adoption of an anaerobic glove box system, we isolated and identified the predominant micro-organisms in lesions of carious dentin from four permanent third molars. The overwhelming majority of the micro-organisms isolated from both shallow and deep layers of carious dentin were obligate anaerobes, suggesting that conditions in the dentin were strictly anaerobic. The predominant obligate anaerobes were Gram-positive rods which were identified as members of the following genera: Propionibacterium, Eubacterium, Arachnia, Lactobacillus, Bifidobacterium, and Actinomyces. Clostridia, Gram-negative rods (Bacteroides and Fusobacterium) and Gram-positive cocci (Peptococcus, Peptostreptococcus, and Streptococcus) were also isolated, although they constituted a minor part of the flora.

show abstract

Characteristics of Propionibacterium and Arachnia isolated from carious dentine.

Cited by 9 publications

References 11 publications

Taxonomic and functional alterations in the salivary microbiota of children with and without severe early childhood caries (S-ECC) at the age of 3

Taxonomic and functional alterations in the salivary microbiota of children with and without severe early childhood caries (S-ECC) at the age of 3

Aerotolerance of Peptostreptococcus anaerobius biofilms

Predominant Obligate Anaerobes in Human Carious Dentin

Contact Info

Product

Resources

About