Characteristics of Polish Isolates of Fusarium sambucinum: Molecular Identification, Pathogenicity, Diversity and Reaction to Control Agents

Baturo‐Cieśniewska, Anna; Lenc, L.; Grabowski, A.; Łukanowski, Aleksander

doi:10.1007/s12230-014-9410-z

Cited by 12 publications

(10 citation statements)

References 54 publications

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“…Du et al, (2012); in their study to identify the Fusarium species obtained from potato tubers infected with dry rot; used 2 primers of the specific TEF-1α gene and obtained ~700 bp band. Diagnostic studies, which obtained 315 bp band by using specific primers of F. sambucinum, were found to be compatible with the diagnostic studies of Ciesniewska et al (2015) and Mishra et al (2003) using species-specific primers of F. sambucinum.…”

Section: Discussionsupporting

confidence: 66%

“…sambucinum, were found to be compatible with the diagnostic studies of Ciesniewska et al (2015) and Mishra et al (2003) using species-specific primers of F. sambucinum.…”

Section: Discussionsupporting

confidence: 61%

“…Firstly, tubers were washed to remove excess soil, then the pieces with healthy and diseased tissue were cut and disinfected in 1% sodium hypochlorite (NaOCl) solution for 3 minutes and rinsed with sterile distilled water and dried. All the sterilized pieces were placed onto PDA and WA, the plates were incubated at 22 ± 2 °C for approximately 7 days (Ciesniewska et al, 2015). After the incubation period, the resulting Fusarium colonies were transferred to fresh PDA plates and maintained at 4 °C for further studies.…”

Section: Sample Collectionmentioning

confidence: 99%

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Characterization of Fusariumsambucinum isolates associated with potato dry rot and evaluation of cultivar susceptibility and fungicides

Yikilmazsoy¹,

Tosun²

2021

Turk J Agric For

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and Salas, 2001). The disease occurs in warehouses. 1 Dry rot disease affects both tubers in the warehouse and seed tuber parts in the field economically (Wharton et al., 2007). The disease begins primarily on the tubers in the form of dark and sunken superficial spots. In tuber flesh, necrotic areas are observed in colors ranging from light brown to black (Figure 1). When the rot progresses, gaps form in the tuber and tuber surface wrinkles.The disease reduces yield, seed quality and marketable product. It causes product losses directly or indirectly. Most tuber infections consist of wounds that appear during planting, mechanical harvesting or classification by size before storage. It is estimated that the crop losses due to dry rot range from 6 to 25%, and during long-term storage, 60% of qualitative tubers can be affected by dry Abstract: Potato dry rot disease (Fusarium spp.) is one of the most important fungal storage rots on potato tubers after harvesting. The aims of this study were to identify the Fusarium species that cause dry rot in potato storages, evaluate the most virulent Fusarium species in vitro and in vivo for fungicide sensitivity and determine the susceptibility of the cultivars. In this context, as a result of surveys carried out in potato storages of Ödemiş and Torbalı counties in İzmir province in 2015-2016; Fusarium species obtained from diseased potato tubers were identified as Fusarium sambucinum, F. oxysporum, F. avenaceum and F. equiseti, using morphological and molecular methods. The most virulent species was determined according to the pathogenicity tests. To determine the susceptibility to disease, 13 potato cultivars, commonly stored in the region throughout the working period, were tested against the most virulent Fusarium species. The pathogenicity tests showed variation between the rank order of susceptibility of the cultivars to the most virulent pathogen. Fungicide sensitivity tests have been conducted with the most susceptible among these cultivars. There are not any plant protection products licensed against this disease in Turkey. On fungicide sensitivity tests, fludioxonil, flutolanil, thiophanate methyl, imazalil, fludioxonil + sedaxane, fluxapyroxad and tolclophos-methyl + thiram efficacy were investigated on the mycelial growth of F. sambucinum. In vitro assays; fludioxonil, fludioxonil + sedaxane and imazalil were the most effective fungicides preventing mycelial growth of the pathogen. In addition to chemicals that are effective in vitro tests, efficacy of Bacillus subtilis QST 713 strain 1.34%, Pseudomonas fluorescens strain Pf1 1.5% and 42% carboxylic acid were also evaluated. In vivo tests were conducted with Russet Burbank, Lady Olympia, Granola cultivars and fungicides were applied in two different methods including before and after inoculation. Results of this study indicated that the treatments with fludioxonil + sedaxane had the highest efficacy and treatments before inoculation was more effective.

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Section: Discussionsupporting

confidence: 66%

“…sambucinum, were found to be compatible with the diagnostic studies of Ciesniewska et al (2015) and Mishra et al (2003) using species-specific primers of F. sambucinum.…”

Section: Discussionsupporting

confidence: 61%

Section: Sample Collectionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Fusariumsambucinum isolates associated with potato dry rot and evaluation of cultivar susceptibility and fungicides

Yikilmazsoy¹,

Tosun²

2021

Turk J Agric For

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“…Fusarium sambucinum Fuckel, F. avenaceum (Fries) Sacc, F. culmorum (W. G. Smith) Saccardo, and F. graminearum Schwabe were reported to be pathogenic on potato tubers in Poland (Baturo-Cieśniewska et al 2015;Stefańczyk et al 2016). However, F. oxysporum that was not pathogenic on potato tubers in laboratory conditions, was the most frequently isolated from diseased tubers by Stefańczyk et al (2016).…”

Section: Introductionmentioning

confidence: 99%

Identification and pathogenicity of Fusarium spp. associated with tuber dry rot and wilt of potato in Algeria

et al. 2021

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Fusarium is one of the most important genera of phytopathogenic fungi, causing potato wilt in the field and potato tuber dry rot during storage. The objectives of this study were to identify Fusarium species associated with both potato diseases in different growing regions in Algeria, and to assess their pathogenicity. Among the 152 isolates collected from symptomatic potato plants and tubers in different provinces in Algeria, 13 species of Fusarium and Neocosmospora were identified. Among these three species were isolated only from plants showing symptoms of Fusarium potato wilt (F. oxysporum, F. venenatum, Neocosmospora solani). Two species (F. culmorum, N. tonkinensis) and an isolate of Neocosmospora sp. were found exclusively in tubers with potato dry rot and the remaining ones (F. redolens, F. cf. tricinctum, F. sambucinum, F. cf. incarnatum-equiseti, F. nygamai, F. brachygibbosum and N. falciformis) were associated with both sample types. Fusarium sambucinum was the most frequent species (52.6% of isolates). Fusarium oxysporum and F. nygamai isolates were the most aggressive in the potato wilt pathogenicity test, and F. sambucinum isolates were the most aggressive in the potato tuber pathogenicity test. This is the first study identifying and characterizing potato dry rot and potato wilt pathogens in Algeria.

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“…Dry rot significantly decreases crop yields, and especially yields of potatoes [21]. In studies by our research team, F. sambucinum was observed to cause dry rot of Zanthoxylum bungeanum stems [22,23,24].…”

Section: Introductionmentioning

confidence: 99%

Structural Characterization of Oligochitosan Elicitor from Fusarium sambucinum and Its Elicitation of Defensive Responses in Zanthoxylum bungeanum

Linhardt

Cao

2016

IJMS

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Oligosaccharide elicitors from pathogens have been shown to play major roles in host plant defense responses involving plant–pathogen chemoperception and interaction. In the present study, chitosan and oligochitosan were prepared from pathogen Fusarium sambucinum, and their effects on infection of Zanthoxylum bungeanum stems were investigated. Results showed that oligochitosan inhibited the infection of the pathogen, and that the oligochitosan fraction with a degree of polymerization (DP) between 5 and 6 showed the optimal effect. Oligochitosan DP5 was purified from fraction DP5-6 and was structurally characterized using electrospray ionization mass spectrometry, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. Oligochitosan DP5 showed significant inhibition against the infection of the pathogenic fungi on host plant stems. An investigation of the mechanism underlying this effect showed that oligochitosan DP5 increased the activities of defensive enzymes and accumulation of phenolics in host Z. bungeanum. These results suggest that oligochitosan from pathogenic fungi can mediate the infection of host plants with a pathogen by acting as an elicitor that triggers the defense system of a plant. This information will be valuable for further exploration of the interactions between the pathogen F. sambucinum and host plant Z. bungeanum.

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Characteristics of Polish Isolates of Fusarium sambucinum: Molecular Identification, Pathogenicity, Diversity and Reaction to Control Agents

Cited by 12 publications

References 54 publications

Characterization of Fusariumsambucinum isolates associated with potato dry rot and evaluation of cultivar susceptibility and fungicides

Characterization of Fusariumsambucinum isolates associated with potato dry rot and evaluation of cultivar susceptibility and fungicides

Identification and pathogenicity of Fusarium spp. associated with tuber dry rot and wilt of potato in Algeria

Structural Characterization of Oligochitosan Elicitor from Fusarium sambucinum and Its Elicitation of Defensive Responses in Zanthoxylum bungeanum

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