Characteristics of Plasma Treated Electrospun Polycaprolactone (PCL) Nanofiber Scaffold for Bone Tissue Engineering

Ko, Yeong-Mu; Choi, Do‐Young; Jung, Sang‐Chul; Kim, Byung–Hoon

doi:10.1166/jnn.2015.8372

Cited by 53 publications

(38 citation statements)

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“…This property, along with good compatibility and easy processing (melting point at 60°C), makes PCL an interesting substrate for tissue engineering (20)(21)(22)(23)(24)(25). However, like other synthetic polymers, PCL also lacks surface wettability and functional surface groups improving the cell attachment that are essential in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

et al. 2016

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Abstract. In this study, biodegradable poly(ε-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO 2 -i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO 2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and Xray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO 2 -i-PCL due to collagen coating and incorporation of TiO 2 , respectively. EDX and XPS studies revealed distribution of entrapped TiO 2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.

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Section: Introductionmentioning

confidence: 99%

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

et al. 2016

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“…The observed reduction in PCL fiber diameters may be due to the etching effects of plasma treatment on polymers [39,40]. Recently, Ko et al reported a similar phenomenon in plasma-treated PCL fibers [39]. Supplementary Fig.…”

Section: Fiber Surface Morphology and Hydrophobicitymentioning

confidence: 96%

“…Compared to plasma-treated PCL and CSS fibers, however, the non-treated PCL fibers had large diameters, which would decrease the overall surface area available for protein immobilization. The observed reduction in PCL fiber diameters may be due to the etching effects of plasma treatment on polymers [39,40]. Recently, Ko et al reported a similar phenomenon in plasma-treated PCL fibers [39].…”

Section: Fiber Surface Morphology and Hydrophobicitymentioning

confidence: 99%

Comparative study of antibody immobilization mediated by lipid and polymer fibers

Cohn

Leung

Zha

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Antibody immobilization and function retention are important to a variety of applications, including proteomics, drug discovery, diagnostics, and biosensors. The present study investigates antibody immobilization mediated by cholesteryl succinyl silane (CSS) fibers, in comparison to hydrophobic polycaprolactone (PCL) fibers and hydrophilic plasma-treated PCL fibers. When incubated with a model protein, the formation of protein aggregates is observed on hydrophobic PCL fibers but not on the more hydrophobic CSS fibers, indicating that CSS fibers immobilize proteins through mechanisms other than hydrophobic interaction. When exposed to a limited amount of antibody, CSS fibers immobilize more antibodies than plasma-treated PCL fibers and no fewer antibodies than PCL fibers. The function retention of antibodies immobilized on the fibers is analyzed using a cell-capture assay, which shows that the antibody-functionalized CSS fibrous matrices capture 6- or 7-fold more cells than the antibody-functionalized PCL or plasma-treated PCL fibrous matrices, respectively. Data collected from the study show that the lipid fiber-mediated immobilization of antibody not only maintains the advantages of physical immobilization such as easiness and rapidness of operation but also improves function retention.

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“…As the material for nanofiber formation, we used the synthetic polyester polycaprolactone known for its biodegradability within several months (Salgado et al 2012) and biocompatibility to hMSC (Valonen et al 2010;Ko et al 2015). The prepared nanofiber membrane from PCL had a basis weight of 36 g/m 2 , thickness of 200 µm and a fibre diameter of 110 ± 40 nm (Plate IX, Fig 1, and Fig 2).…”

Section: Preparation Of the Organic Carriermentioning

confidence: 99%

Three-dimensional bone tissue substitute based on a human mesenchymal stem cell culture on a nanofiber carrier and inorganic matrix

et al. 2016

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The aim was to construct a composite structure for bone tissue substitute on the basis of a degradable composite of an organic nanofiber carrier and an inorganic matrix in 3D, and to achieve subsequent colonisation by differentiated human mesenchymal stem cells (hMSC) towards osteocytes. We developed an active bone tissue substitute using nanofiber technology for a polycaprolactone (PCL) scaffold with the addition of hydroxyapatite and the colonisation of both components with hMSC with the ability of differentiation towards osteocytes. The constructed composition included the components necessary for bone healing (inorganic and cellular) and it also forms a spatially-oriented 3D structure. We used polycaprolactone Mw 70,000 with electrostatic spinning for the formation of nanofibers using a modified Nanospider TM method. For the inorganic component we used orthophosphate-calcium silicate with a crystal size of 1-2 mm which the nanofiber membrane was coated with. Both components were connected together with a tissue adhesive based of fibrin glue. Cultivated hMSC cells at a concentration of 1.2 × 10 4 /cm 2 were multiplied in vitro and then cultivated in the expansion medium. HMSC overgrew both the PCL membrane and the Si-CaP crystals. After colonisation with cultivated cells, this composite 3D structure can serve as a three-dimensional bone tissue replacement.

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Characteristics of Plasma Treated Electrospun Polycaprolactone (PCL) Nanofiber Scaffold for Bone Tissue Engineering

Cited by 53 publications

References 1 publication

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

Comparative study of antibody immobilization mediated by lipid and polymer fibers

Three-dimensional bone tissue substitute based on a human mesenchymal stem cell culture on a nanofiber carrier and inorganic matrix

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