Characteristics of papillary collecting duct cells in primary culture

Husted, Russell F.; Hayashi, Matsuhiko; Stokes, John B.

doi:10.1152/ajprenal.1988.255.6.f1160

Cited by 39 publications

(52 citation statements)

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“…CD cells were isolated from β1 flox/flox mice following the methodology described by Husted et al (Husted et al, 1988), and β1 was deleted by infecting the cells with an adenocre virus in vitro. To verify adequate deletion of β1 integrin, the cells were subjected to flow cytometry as described below.…”

Section: Generation Of Integrin β1-null Cell Linementioning

confidence: 99%

β1 integrin is necessary for ureteric bud branching morphogenesis and maintenance of collecting duct structural integrity

et al. 2009

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The kidney collecting system develops from branching morphogenesis of the ureteric bud (UB). This process requires signaling by growth factors such as glial cell line derived neurotrophic factor (GDNF) and fibroblast growth factors (FGFs) as well as cell extracellular matrix interactions mediated by integrins. The importance of integrin signaling in UB development was investigated by deleting integrin β1 at initiation (E10.5) and late (E18.5) stages of development. Deletion at E10.5 resulted in a severe branching morphogenesis phenotype. Deletion at E18.5 did not alter renal development but predisposed the collecting system to severe injury following ureteric obstruction. β1 integrin was required for renal tubular epithelial cells to mediate GDNF-and FGF-dependent signaling despite normal receptor localization and activation in vitro. Aberrations in the same signaling molecules were present in the β1-null UBs in vivo. Thus β1 integrins can regulate organ branching morphogenesis during development by mediating growthfactor-dependent signaling in addition to their well-defined role as adhesion receptors.

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Section: Generation Of Integrin β1-null Cell Linementioning

confidence: 99%

β1 integrin is necessary for ureteric bud branching morphogenesis and maintenance of collecting duct structural integrity

et al. 2009

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“…CD cells were isolated from ILK1 flox mice following the methodology described by Husted et al (Husted et al, 1988) and ILK was deleted by infecting the cells with an adenocre virus in vitro. Deletion of ILK was verified by immunoblotting.…”

Section: Generation Of Ilk-null Cell Linementioning

confidence: 99%

Integrin-linked kinase regulates p38 MAPK-dependent cell cycle arrest in ureteric bud development

Smeeton

Zhang

Bulus³

et al. 2010

Development

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SUMMARYThe integrin-linked kinase (ILK), pinch and parvin ternary complex connects the cytoplasmic tails of 1 integrins to the actin cytoskeleton. We recently showed that constitutive expression of ILK and alpha parvin in both the ureteric bud and the metanephric mesenchyme of the kidney is required for kidney development. In this study, we define the selective role of ILK in the ureteric bud of the mouse kidney in renal development by deleting it in the ureteric cell lineage before the onset of branching morphogenesis (E10.5). Although deleting ILK resulted in only a moderate decrease in branching, the mice died at 8 weeks of age from obstruction due to the unprecedented finding of intraluminal collecting duct cellular proliferation. ILK deletion in the ureteric bud resulted in the inability of collecting duct cells to undergo contact inhibition and to activate p38 mitogen-activated protein kinase (MAPK) in vivo and in vitro. p38 MAPK activation was not dependent on the kinase activity of ILK. Thus, we conclude that ILK plays a crucial role in activating p38 MAPK, which regulates cell cycle arrest of epithelial cells in renal tubulogenesis.

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“…For each isolation used for mRNA analysis, parallel cultures were made in the large and the small Millicell filters. The large filters were used for RNA isolation and the small filters used for the electrical measurements to be sure that the values were typical of those we have previously reported (1,2,35,36 (40). Cell-attached patches were used to detect channel activity in intact cells.…”

Section: Introductionmentioning

confidence: 99%

rENaC is the predominant Na+ channel in the apical membrane of the rat renal inner medullary collecting duct.

Volk

Sigmund²,

Snyder³

et al. 1995

J. Clin. Invest.

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The terminal nephron segment, the inner medullary collecting duct (IMCD), absorbs Na+ by an electrogenic process that involves the entry through an apical (luminal) membrane Na+ channel. To understand the nature of this Na+ channel, we employed the patch clamp technique on the apical membrane of primary cultures of rat IMCD cells grown on permeable supports. We found that all ion channels detected in the cell-attached configuration were highly selective for Na+ (Li') over K+. The open/closed transitions showed slow kinetics, had a slope conductance of 6-11 pS, and were sensitive to amiloride and benzamil. Nonselective cation channels with a higher conductance (25-30 pS), known to be present in IMCD cells, were not detected in the cell-attached configuration, but were readily detected in excised patches. The highly selective channels had properties similar to the recently described rat epithelial Na + channel complex, rENaC. We therefore asked whether rENaC mRNA was present in the IMCD. We detected mRNA for all three rENaC subunits in rat renal papilla and also in primary cultures of the IMCD. Either glucocorticoid hormone or mineralocorticoid hormone increased the amount of a-rENaC subunit mRNA but had no effect on the mRNA level of the 8-rENaC or y-rENaC subunits. From these data, taken in the context of other studies on the characteristics of Na+ selective channels and the distribution of rENaC mRNA, we conclude that steroid stimulated Na + absorption by the IMCD is mediated primarily by Na + channels having properties of the rENaC subunit complex. (J. Clin. Invest. 1995. 96:2748-2757

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Characteristics of papillary collecting duct cells in primary culture

Cited by 39 publications

References 0 publications

β1 integrin is necessary for ureteric bud branching morphogenesis and maintenance of collecting duct structural integrity

β1 integrin is necessary for ureteric bud branching morphogenesis and maintenance of collecting duct structural integrity

Integrin-linked kinase regulates p38 MAPK-dependent cell cycle arrest in ureteric bud development

rENaC is the predominant Na+ channel in the apical membrane of the rat renal inner medullary collecting duct.

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