Characteristics of nonalcoholic fatty liver disease induced in wistar rats following four different diets

Abstract

BACKGROUND/OBJECTIVESThe prevalence of nonalcoholic fatty liver disease (NAFLD) has increased worldwide in parallel with overnutrition characterized by high-fat and high-carbohydrate intake. Our objective was to establish, in 16 weeks, a model of NAFLD in Wistar pathogen-free rats following four dietary types.MATERIALS/METHODSForty (6 weeks old) healthy Wistar male rats, weighing an average of 150 g were randomly divided into four groups of ten and assigned a diet with the same quantity (15 g/rat/day), but with different composition. The moderate-fat (MF) group was fed a moderate-fat diet (31.5% fat and 50% carbohydrates), the high-fat (HF) group was fed a fat-rich diet (51% fat), the high-sucrose (HS) group and the high-fructose (HFr) group were fed a carbohydrate-rich diet (61%). The carbohydrate contents of the HS group was composed of 60.3% sucrose while that of the HFr group was composed of 59.3% fructose.RESULTSAt week 16, the HF group had the highest percentage of cells enriched in fat (40%) and the highest weight and liver weight (P < 0.05). The HFr group showed significantly higher levels of serum triglycerides, alanine aminotransferase and adiponectin at week 16 as compared to week 1 (P < 0.05).CONCLUSIONSThe 15 g/rat/day diet composed of 51% fat or 61% carbohydrates enriched mainly in fructose may induce characteristics of NAFLD in rats.

Comparison of Three Different Diet-Induced Non Alcoholic Fatty Liver Disease Protocols in Rats: A Pilot Study

“…Although the high fructose group showed significantly higher levels of serum alanine aminotransferase and triglycerides. 26 In this study, we did not observe any significant differences in final body weights and liver enzyme levels between groups that may be because of small sample size. Somewhat contrary to our results, Zhang et al (2014) who used a diet containing 15% fat, 15% sucrose and 2% cholesterol for 8 weeks, as a dietary model of NAFLD, reported increased food intake, body and liver weights as well as macrovesicular steatosis compared with control group.…”

Animal models of non‐alcoholic fatty liver disease: current perspectives and recent advances

“…For example, Wistar male rats were fed diets with the same quantity (15 g/rat per day) for 16 weeks but with different compositions, including high‐fat, moderate‐fat, high‐sucrose and high‐fructose groups. The high‐fat group had the highest body and liver weights, and the highest percentage of liver steatosis (40%) .…”

The role of cannabinoid receptor 1 in the development of oxidative/nitrosative stress in mice with non-alcoholic fatty liver disease

“…Razvoj oksidativnog stresa u našoj studiji potvrđen je porastom koncentracije MDA u jetri miševa koji su bili na dijeti bogatoj mastima (grafikon 2). Naši rezultati su u saglasnosti sa rezultatima studije koju su sproveli Fakhoury-Sayegh i sar., koji su na istom eksperimentalnom modelu pokazali da HFD dovodi do razvoja NAFLD i hepatocelularnog oštećenja (20). Uzimajući u obzir predloženu hipotezu "dva udara", rezultati studije koju su sproveli Jorgačević i sar.…”

“…S tim u vezi, Fakhoury-Sayegh i sar. pokazali su da se u toku NASH mitohondrijalna disfunkcija s vremenom samo pogoršava, i to usled strukturnih promena u mitohondrijalnoj membrani, kao i respiratornom lancu (24). Sledeći značajan izvor ROS u NAFLD predstavlja povećana ekspresija gena za CYP2E1.…”