Characteristics of newly‐formed cementum following emdogain application

Abstract

Periodontal regenerative techniques have been proposed; however, the outcomes remain debatable. The present investigation assessed the regenerated cementum following enamel matrix derivative application in dehiscence-type defects. Buccal osseous dehiscences were surgically created on the maxillary cuspid, and the second and fourth premolars in five female beagle dogs. The treatment group (n=15 sites) received the enamel matrix derived application, whereas the control groups (n=15) did not. The dogs were sacrificed 4 months following treatment and the specimens were histologically and histometrically examined. The newly formed cementum was uneven in thickness and mineralization, overlapped the old cementum and exhibited functional orientation, cementocyte lacunae and collagen fibril bundles. Most of the histological specimens showed the presence of a gap between the newly formed cementum and the underlying dentin. Control sites did not exhibit any cementum formation. The present study concluded that newly formed cementum is of cellular type and exhibits multiple characteristics.

Effect of enamel matrix derivative protein on the healing of standardized epithelial wounds: a histomorphometric analysis in vivo

“…Amelogenin, a 20 kDa hydrophobic protein, is the chief ingredient in the EMD. Studies have shown that amelogenin promotes healing and regeneration of the supporting structures of teeth including cementum (3,4). Similar results were reported by Berlucchi et al (5).…”

“…Previous studies (3,9,10) have largely focused on the ability of EMD to repair and regenerate periodontal tissues; however, results from the present experimental study showed that the benefits of EMD are not only restricted to oral rehabilitation but can augment healing of external skin wounds as well. After a vigilant review of the pertinent indexed literature, we observed that not many studies have addressed the impact of EMD on skin wounds.…”

Effects of conservatively treated diseased cementum with or without EMD on in vitro cementoblast differentiation and in vivo cementum‐like tissue formation of human periodontal ligament cells

“…However, surprisingly, there was no NFC formation on dentin slices, whether they had been treated with EMD or not. This is not consistent with some previous studies in which EMD induced new cementum formation on dentin surfaces following GTR . In our previous investigation on healthy root slices, it was also found that there was NFC formation on dentin slices, although the number of specimens with NFC formation was much lower than that on cementum slices .…”

Effects of cathepsin K on Emdogain-induced hard tissue formation by human periodontal ligament stem cells

“…The results revealed that EMD could promote PDL regeneration, and acellular cementum could be observed under the newly formed fibres. Another study treated alveolar bone defect areas with EMD (Al‐Hezaimi et al ., ); cementoblasts were observed in the newly formed cementum.…”

“…It has been used in the clinical treatment of periodontitis since 1997 (Heijl et al ., ). Histological studies in both animals and humans have provided evidence for alveolar bone and cementum regeneration following treatment with EMD (Al‐Hezaimi et al ., ; Baltacioglu et al ., ; Bosshardt et al ., ; Watanabe et al ., ), and there is substantial evidence that EMD can regulate the expression of genes crucial for both cementogenic and osteogenic differentiation (Tokiyasu et al ., ). Interestingly, the inhibition of the amelogenin fraction within the homogenized enamel matrix that is responsible for the biological effect of EMD in inducing hard tissue (alveolar bone and cementum) regeneration, the volume of newly formed bone and cementum decreased significantly in a buccal dehiscence model in monkeys (Hammarstrom et al ., ).…”