Characteristics of new <i>Staphylococcus aureus</i>-RBC adhesion mechanism independent of fibrinogen and IgG under hydrodynamic shear conditions

Shin, Pilsoo; Pawar, Parag D.; Κωνσταντόπουλος, Κωνσταντίνος; Ross, Julia M.

doi:10.1152/ajpcell.00034.2005

Cited by 13 publications

(13 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although S. aureus strains depending on external thiamine supply have been observed [35], there are no reports of thiamine uptake capabilities to date, which would – in additional to de novo synthesis - feed the intracellular thiamine pool in S. aureus . Interestingly, S. aureus is known to be adhesive to erythrocytes - a source of thiamine [36], [37] - which might emphasise uptake of this nutrient from red blood cells.…”

Section: Resultsmentioning

confidence: 99%

The Vitamin B1 Metabolism of Staphylococcus aureus Is Controlled at Enzymatic and Transcriptional Levels

et al. 2009

View full text Add to dashboard Cite

Vitamin B1 is in its active form thiamine pyrophosphate (TPP), an essential cofactor for several key enzymes in the carbohydrate metabolism. Mammals must salvage this crucial nutrient from their diet in order to complement the deficiency of de novo synthesis. In the human pathogenic bacterium Staphylococcus aureus, two operons were identified which are involved in vitamin B1 metabolism. The first operon encodes for the thiaminase type II (TenA), 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase (ThiD), 5-(2-hydroxyethyl)-4-methylthiazole kinase (ThiM) and thiamine phosphate synthase (ThiE). The second operon encodes a phosphatase, an epimerase and the thiamine pyrophosphokinase (TPK). The open reading frames of the individual operons were cloned, their corresponding proteins were recombinantly expressed and biochemically analysed. The kinetic properties of the enzymes as well as the binding of TPP to the in vitro transcribed RNA of the proposed operons suggest that the vitamin B1 homeostasis in S. aureus is strongly regulated at transcriptional as well as enzymatic levels.

show abstract

Section: Resultsmentioning

confidence: 99%

The Vitamin B1 Metabolism of Staphylococcus aureus Is Controlled at Enzymatic and Transcriptional Levels

et al. 2009

View full text Add to dashboard Cite

show abstract

“…As for the majority of in vitro experiments, the transferability of our results is challenged by issues that need to be addressed in further studies. The experiments were performed using isolated PMNs instead of whole blood which artificially excludes disruptive factors, such as S. aureus binding to erythrocytes and platelets [48, 49]. The chosen assay medium can influence the functionality and viability of PMNs; however our choice, KRb, is supported by a recent publication by Alhede et al [50].…”

Section: Discussionmentioning

confidence: 99%

Distinct contribution of hyperbaric oxygen therapy to human neutrophil function and antibiotic efficacy against Staphylococcus aureus

Schwartz

Lerche

Christophersen

et al. 2021

APMIS

View full text Add to dashboard Cite

Staphylococcus aureus (SA) causes superficial and severe endovascular infections. The present in vitro study investigates the anti‐SA mechanisms of hyperbaric oxygen therapy (HBOT) on direct bacterial killing, antibiotic potentiation, and polymorphonuclear leukocyte (PMN) enhancement. SA was exposed to isolated human PMNs, tobramycin, ciprofloxacin, or benzylpenicillin. HBOT was used as one 90‐min session. Bacterial survival was evaluated after 4 h by quantitative bacteriology. PMN functionality as reactive oxygen species (ROS) production was measured by means of dihydrorhodamine 123 analysis. We showed that HBOT exhibits significant direct anti‐SA effects. HBOT increased the anti‐SA effects of PMNs by 18% after PMA stimulation (p = 0.0004) and by 15% in response to SA (p = 0.36). HBOT showed an additive effect as growth reductions of 26% to sub‐MICs of tobramycin (p = 0.0057), 44% to sub‐MICs of ciprofloxacin (p = 0.0001), and 26% to sub‐MICs of penicillin (p = 0.038). The present in vitro study provides evidence that HBOT has differential mechanisms mediating its anti‐SA effects. Our observation supports the clinical possibility for adjunctive HBOT to augment the host immune response and optimize the efficacy of antibiotic treatments.

show abstract

“…The head domain, once assembled, then adheres to the host ECM via, for example, collagen, vitronectin or fibronectin [ 58 ]. Recent work showed that different adhesins bind differently to ECM components and that binding is dramatically influenced by shear forces [ 56 , 61 , 62 , 63 ]. In general, improving our understanding of adhesin–receptor interaction requires more detailed insights into their structural aspects [ 64 ].…”

Section: Microbial Adhesin–receptor Pairsmentioning

confidence: 99%

Host-Pathogen Adhesion as the Basis of Innovative Diagnostics for Emerging Pathogens

Belkum¹,

Almeida²,

Bardiaux

et al. 2021

Diagnostics

View full text Add to dashboard Cite

Infectious diseases are an existential health threat, potentiated by emerging and re-emerging viruses and increasing bacterial antibiotic resistance. Targeted treatment of infectious diseases requires precision diagnostics, especially in cases where broad-range therapeutics such as antibiotics fail. There is thus an increasing need for new approaches to develop sensitive and specific in vitro diagnostic (IVD) tests. Basic science and translational research are needed to identify key microbial molecules as diagnostic targets, to identify relevant host counterparts, and to use this knowledge in developing or improving IVD. In this regard, an overlooked feature is the capacity of pathogens to adhere specifically to host cells and tissues. The molecular entities relevant for pathogen–surface interaction are the so-called adhesins. Adhesins vary from protein compounds to (poly-)saccharides or lipid structures that interact with eukaryotic host cell matrix molecules and receptors. Such interactions co-define the specificity and sensitivity of a diagnostic test. Currently, adhesin-receptor binding is typically used in the pre-analytical phase of IVD tests, focusing on pathogen enrichment. Further exploration of adhesin–ligand interaction, supported by present high-throughput “omics” technologies, might stimulate a new generation of broadly applicable pathogen detection and characterization tools. This review describes recent results of novel structure-defining technologies allowing for detailed molecular analysis of adhesins, their receptors and complexes. Since the host ligands evolve slowly, the corresponding adhesin interaction is under selective pressure to maintain a constant receptor binding domain. IVD should exploit such conserved binding sites and, in particular, use the human ligand to enrich the pathogen. We provide an inventory of methods based on adhesion factors and pathogen attachment mechanisms, which can also be of relevance to currently emerging pathogens, including SARS-CoV-2, the causative agent of COVID-19.

show abstract

Characteristics of new Staphylococcus aureus-RBC adhesion mechanism independent of fibrinogen and IgG under hydrodynamic shear conditions

Cited by 13 publications

References 51 publications

The Vitamin B1 Metabolism of Staphylococcus aureus Is Controlled at Enzymatic and Transcriptional Levels

The Vitamin B1 Metabolism of Staphylococcus aureus Is Controlled at Enzymatic and Transcriptional Levels

Distinct contribution of hyperbaric oxygen therapy to human neutrophil function and antibiotic efficacy against Staphylococcus aureus

Host-Pathogen Adhesion as the Basis of Innovative Diagnostics for Emerging Pathogens

Contact Info

Product

Resources

About