Characteristics of Neuropeptide-Cytokine Immunity Links in Patients With Combined Cardiovascular Pathology, Proceeding With Anxiety/Depression Disorder

Герцев, А. В.; Закревский, Ю. Н.

doi:10.15789/1563-0625-2018-2-277-284

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“…Цитокины -семейство полипептидных молекул, которые синтезируются клетками различных тканей организма, наиболее активными продуцентами этих молекул являются иммунокомпетентные клетки. ЦК регулируют ряд нормальных физиологических функций, защитные реакции при внедрении различных патогенов, а также развитие большинства патологических процессов, включая канцерогенез, сердечно-сосудистые нарушения, аутоиммунную патологию, аллергические реакции и другие [4,31,40,49]. В отношении регуляции воспалительного процесса ЦК разделяют на провоспалительные (инициирующие и усиливающие воспалительную реакцию) и противовоспалительные (подавляющие воспаление).…”

Section: цитокины при ювенильных артритахunclassified

Сoncentration of Anti-Inflamatory Cytokines in Cell Culture Supernatants in Children With Juvenile Idiopathic Arthritis

2019

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Juvenile idiopathic arthritis is a chronic inflammatory disease of the joints in children, mainly of autoimmune or auto-inflammatory nature. It is a heterogeneous group, which includes different subtypes of the disease. Different mechanisms may play role in the pathogenesis of distinct subtypes of juvenile arthritis. However, a long-term imbalance of pro- and anti-inflammatory cytokines is important for all subtypes of disease. The aim of the present study was to determine spontaneous and stimulated anti-inflammatory cytokines production by peripheral blood cells from the children with juvenile idiopathic arthritis. Patients of 2 to 17 years old with different subtypes of juvenile idiopathic arthritis (n = 99) and healthy children without signs of autoimmune diseases (control, n = 31) were examined. Spontaneous and phytohemagglutinin-stimulated concentrations of IL-1ra, IL-4, IL-10, TGF-β in supernatants of whole-blood cultures were determined by ELISA. Differences in the spontaneous and mitogen-stimulated secretion of the cytokines in patients with different subtypes of juvenile arthritis have not been revealed. The spontaneous IL-1ra, IL-4 and IL-10 production by blood cells in the common group of patients with juvenile idiopathic arthritis was similar to the controls. The median value of spontaneous TGF-β concentration in the patients was below the detection level, whereas blood cells of healthy children had a higher potential of spontaneous TGF-β production. IL-4 and IL-10 production after incubation of peripheral blood cells with phytohemagglutinin in patients and in the control group did not differ from the controls, while IL-1ra and TGF-β synthesis was significantly lower than in healthy children.The spontaneous and/or stimulated production of IL-1ra, TGF-β by blood cells in children with juvenile idiopathic arthritis reflects the pathogenic significance of these cytokines in disease. Stimulation of cells can reveal a latent deficiency in the synthesis of cytokines, which is not evident when determining its concentration in serum or supernatants of spontaneous whole-blood cultures.

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Section: цитокины при ювенильных артритахunclassified