Characteristics of Mouse Lymphoid Cells Proliferating in vitro by Recombinant Human Interleukin 2 (TGP‐3): Comparison between Normal and Nude Mice

Abstract

Various lymphoid cells obtained from BALB/c and BALB/c nu/nu mice were cultured in vitro with recombinant human interleukin 2 (rIL 2), and the characteristics of responder cells to rIL 2 were analyzed. Spleen cells, lymph node cells, and thymocytes except for bone marrow cells obtained from BALB/c mice remarkably proliferated in response to rIL 2. On the other hand, among lymphoid cells obtained from BALB/c nu/nu mice, only lymph node cells showed significant proliferation by rIL 2. Flow cytometric analyses revealed that mainly two types of lymphoid cells were proliferating in response to rIL 2 in BALI* mice, i.e., Thy 1+, Lyt 1-, Lyt 2-and Thy 1+, Lyt 1-, Lyt 2+ cells. On the other hand, most of the proliferating cells were Thy 1+, Lyt 1-, Lyt 2-cells in BALB/c nu/nu mice. Treatment with various antibodies plus complement revealed that the majority of IL 2-responsive cells in BALB/c mice were Thy 1+, Lyt 1+, and Lyt 2+, although a minor part of them were Thy 1-, Lyt 1-, and Lyt 2-. On the other hand, a predominant type of the IL 2-responsive cells in BALB/c nu/nu mice were Thy 1-, Lyt 1-, and Lyt 2-, though some were Thy 1+. Nonspecific killer activity against tumor cells increased to variable extents in all of the lymphoid cells of both strains after culture with rIL 2. Our results indicate that mouse responder cells to rIL 2 have the following characteristics. First, the responder cells exist abundantly among spleen, lymph nodes, and thymus in normal mice, though their cell lineages are heterogeneous; one is of T cell lineage and the other of natural killer (NK) cell lineage. Second, nude mice are defective in the responder cells of T cell lineage but not of NK cell lineage. Moreover, the responder cells in nude mice predominantly accumulate in the lymph nodes but not other lymphoid organs.Since Morgan et al first reported a lymphokine as a growth factor to T cells (16), numerous findings on interleukin 2 (IL 2) have appeared (11,27). Nowadays, the importance of IL 2 in the immune system is widely accepted, although the nature of responder cells to IL 2 remains to be fully characterized. In early studies, IL 2 was thought to support only the growth of T cells stimulated with lectins or antigens (5, 16), but recent studies have demonstrated that IL 2 regulates proliferation and differentiation in not only T cells but also other cells such as B cells, NK cells, and 761

Nanoparticles Containing Curcumin Useful for Suppressing Macrophages In Vivo in Mice

“…We subsequently examined which type of cells incorporated curcumin/liposome, by means of cell surface antigens [CD90.2 (Thy 1.2), F4/80, CD36, and CD11b]. Thy1 is a representative T cell maker [ 23 ]. On the other hand, F4/80, CD36, and CD11b are known as macrophage cell surface makers [ 25 , 26 ].…”

“…Peritoneal cells were pooled from three mice in each group 48 h after the last injection. Cell staining and flow cytometric analyses were performed with Guava easyaCyte (Merck) according to the method as described previously [ 23 ]. Peritoneal cells (1 × 10 7 /ml) were stained with Alexa Fluor 488 anti-mouse CD90.2 (Thy-1.2) Antibody(BioLegend)at 2.5 μg/ml, fluorescein isothiocyanate (FITC) rat anti-mouse F4/80(AbD serotec) at 10 μg/ml, Alexa Fluor 488 anti-mouse CD36 Antibody (BioLegend) at 2.5μg/ml, and PerCP/Cy5.5 anti-mouse/human CD11b (BioLegend) at 2.5 μg/ ml, respectively.…”

Loss-of-function Mutations K11E or E271K Lead to Novel Tumor Suppression, Implicate Nucleolar Helicase DDX24 Oncogenicity

“…Unconventional T cells including natural killer T cells, mucosal-associated invariant T cells and γδ T cell were thymus-independent and made up ~10% of circulating T cells 45 . Given nude mice were not defective in the responder cells of NK cell lineage 46 , we speculated that K11E-DDX24 or E271K-DDX24 may enhance non-specific immune killing to inhibit cell proliferation and tumor growth.…”