Characteristics of methanogens isolated from bovine rumen

Miller, T.; Wolin, M. J.; Zhao, Hongxue; Bryant, M. P.

doi:10.1128/aem.51.1.201-202.1986

Cited by 70 publications

(20 citation statements)

References 6 publications

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“…In the present study, the observed promotion of the methanogenic population in diets supplemented with C 8 : 0 and C 10 : 0 may be due to the decline in numbers of protozoa, because these microorganisms also degrade Archaea to a certain extent (Newbold et al 1996). Moreover, Miller et al (1986) documented a stimulation of M. ruminantium growth by a mixture of isoacids, including isovalerate, which increased with C 8 : 0 treatment (Table 1).…”

Section: Fatty Acids and Microbial Populationssupporting

confidence: 53%

Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets

Dohme¹,

Machmüller²,

Wasserfallen³

et al. 2001

Lett Appl Microbiol

141

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The objective of the present study was to investigate the effects of seven different pure fatty acids on rumen fermentation using the rumen simulation technique (RUSITEC). The fatty acids were supplied to a complete ruminant diet at a proportion of 50 g x kg(-1) dietary dry matter and compared with an unsupplemented control. Methane release and methanogenic counts were suppressed by the fatty acids C12 : 0, C14 : 0 and C18 : 2 whereas C8 : 0, C10 : 0, C16 : 0 and C18 : 0 showed no corresponding effects. Apart from C12 : 0 and C18 : 2, C8 : 0 and C10 : 0 also adversely affected ciliate protozoa suggesting independence from the methane-suppressing effect of medium-chain fatty acids (MCFA). Although MCFA but not C18 : 2 reduced ruminal fibre degradation, the influence on other fermentation traits remained low. In conclusion, the supply of certain fatty acids to ruminant diets seems to have the potential to reduce methane release.

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Section: Fatty Acids and Microbial Populationssupporting

confidence: 53%

Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets

Dohme¹,

Machmüller²,

Wasserfallen³

et al. 2001

Lett Appl Microbiol

141

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“…users ml-l. The H2 users seen in positive tubes were all of the Methanobrevibacter morphotype (5). The dominant morphotype in the methanol MPN estimates was that of E. limosum, except that most of the tubes inoculated with the tion dilution contained clumps of the Methanosarcina morphotype.…”

Section: Resultsmentioning

confidence: 91%

Quin's oval and other microbiota in the rumens of molasses-fed sheep

Vicini¹,

Brulla²,

Davis³

et al. 1987

Appl Environ Microbiol

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Two rumen-cannulated wether sheep were fed a diet containing 1 kg of a liquid-molasses mixture, 80 g of soybean oil meal, and 100 g of chopped wheat straw once a day. In 6 weeks and thereafter, the microbiota adapted such that Quin's oval, a very large bacterium, was present in huge numbers (11.3 x 1010 and 1.3 x 1010 ml-' after 73 days). Direct microscopic counts were also done on small bacteria, moderate-sized Selenomonas spp., and small Entodinium spp., which were the only protozoa seen. After the necessary dilution of rumen contents to make the microbial cells visible, Quin's ovals were seen to be much smaller in sheep 1 than in sheep 2. Most-probable-number estimates indicated that Methanobrevibacter spp. were present at 107 ml-', Methanosarcina spp. were present at 103 ml-', and Eubacterium limosum-like bacteria were present at 105 to

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“…stadtmanae and Mbb. smithii were species commonly reported in various studies from beef cattle and dairy cows by either culture‐based methods (Miller et al. 1986; Miller and Lin 2002) or molecular identification (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Owing to their fastidious nature, only seven species of methanogens have been isolated from the rumen: Methanobacterium bryantii , Methanobacterium formicicum , Methanobrevibacter millerae , Methanobrevibacter olleyae , Methanobrevibacter ruminantium , Methanomicrobium mobile and Methanosarcina barkeri (Oppermann et al. 1957; Paynter and Hungate 1968; Miller et al. 1986; Jarvis et al.…”

Section: Introductionmentioning

confidence: 99%

Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive

Zhou

Chung

Beauchemin

et al. 2011

Journal of Applied Microbiology

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Aims: To investigate the relationship between ruminal methanogen community and host enteric methane (CH4) production in lactating dairy cows fed diets supplemented with an exogenous fibrolytic enzyme additive. Methods and Results: Ecology of ruminal methanogens from dairy cows fed with or without exogenous fibrolytic enzymes was examined using PCR–denaturing gradient gel electrophoresis (PCR–DGGE) analyses and quantitative real‐time PCR (qRT‐PCR). The density of methanogens was not affected by the enzyme additive or sampling times, and no relationship was observed between the total methanogen population and CH4 yield (as g per head per day or g kg−1 DMI). The PCR–DGGE profiles consisted of 26 distinctive bands, with two bands similar to Methanogenic archaeon CH1270 negatively correlated, and one band similar to Methanobrevibacter gottschalkii strain HO positively correlated, with CH4 yield. Three bands similar to Methanogenic archaeon CH1270 or Methanobrevibacter smithii ATCC 35061 appeared after enzyme was added. Conclusions: Supplementing a dairy cow diet with an exogenous fibrolytic enzyme additive increased CH4 yield and altered the composition of the rumen methanogen community, but not the overall density of methanogens. Significance and Impact of the Study: This is the first study to identify the correlation between methanogen ecology and host CH4 yield from lactating dairy cows.

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Characteristics of methanogens isolated from bovine rumen

Cited by 70 publications

References 6 publications

Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets

Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets

Quin's oval and other microbiota in the rumens of molasses-fed sheep

Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive

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