Characteristics of Lactoferrin Receptor in Bovine Intestine: Higher Binding Activity to the Epithelium Overlying Peyer's Patches

Abstract

Several lines of evidence have recently demonstrated the occurrence of specific lactoferrin (Lf) receptors in different cells. We report here, for the first time, the characteristics of binding, and distribution of Lf receptors in the bovine intestinal tract with special emphasis on the epithelium overlying Peyer's patches (EOPP). Brush-border membrane vesicles (BBMV) were prepared from the mucosa of duodenum, jejunum, ileum, colon, EOPP in jejunum and EOPP in ileum. Receptor binding assays were carried out using 125I-labelled bovine Lf. Specific and saturable Lf receptors were found in BBMV of all the intestinal segments examined. Non-linear regression and Scatchard plot analyses clearly revealed that EOPP had the highest binding maximal (Bmax), and lowest in colon. The maximum dissociation constant (Kd) 3.74 microm was in the ileum. We found that bovine transferrin competed with Lf for the same binding site of receptors. In contrast, no binding of bovine serum albumin occurred. It was concluded that Lf receptors in the mucosal lining are attributable to mediate multifunctional activities of Lf in the gut, especially in the EOPP.

Receptor-Mediated Transport of Lactoferrin into the Cerebrospinal Fluid via Plasma in Young Calves

“…Macromolecules are transported from the gut to the systemic circulation either by specific receptor-mediated transcytosis or nonspecific transcytosis [29]. Nevertheless, IgG is not absorbed after the "Gut closure" in the intestines, but Lf-R are present in the intestines of different species, such as mice [18], piglets [13], monkeys [5], humans [20] and cows [34]. Together these studies suggest that Lf absorption in the gut involves receptor-mediated transcytosis.…”

“…Iodination of Lf: Labeling of Lf with 125 I was performed in the presence of iodogen as a catalyst, by the method of Fraker and Speck [12] and described by Talukder et al [34].…”

“…To explore the transportation phenomenon, a binding assay for Lf-R was considered. In addition, intestinal mucosal brush border membrane vesicles (BBMV) contained Lf-R in different species, such as piglets [13], humans [20] and adult cows [34]. Therefore, an Lf-binding assay of the choroid plexus was performed.…”

Oral Lactoferrin Results in T Cell–Dependent Tumor Inhibition of Head and Neck Squamous Cell Carcinoma In vivo

“…The lactoferrin receptor is expressed in many mammalian tissues, including intestine, heart, spleen, liver, monocytes, lymphocytes, platelets, and salivary glands (27 -30). The lactoferrin receptors found in the intestinal epithelium overlying peyers patches have the highest specific binding capability when compared with lactoferrin receptors at other sites (30). The murine lactoferrin receptor has 87% homology with the human receptor and shows binding specific for human, mouse, and bovine lactoferrin (28,31,32).…”

Trans-Endothelial and Trans-Epithelial Transfer of Lactoferrin into the Brain through BBB and BCSFB in Adult Rats

“…As well, orally administered LF was reported to be absorbed into systemic circulation not only in neonates [6,14], but also in weaned pigs [5] and young cattle [16]. Talukder et al [15] reported the detection of a specific LF-receptor in adult cow intestines, including the duodenum, jejunum, ileum, and colon, and Peyer's patches in the jejunum and ileum. Interestingly, the density of LFreceptor was higher in Peyer's patches than in other regions of the intestine.…”