Characteristics of intestinal microbiota in infants with late-onset breast milk jaundice

Abstract

IntroductionIn this paper, microbiota analysis was determined to analyze the structure and difference of intestinal microbiota between LBMJ (late-onset breast milk jaundice) infants and healthy individuals.MethodsWe collected fresh fecal samples from 13 infants with LBMJ and 13 healthy individuals, then determined the intestinal microbiota by 16 s rRNA sequencing. The differences of microbiota structure, diversity and functional characteristics between the two groups were analyzed, and the correlation between dominant genus and TcB (transcutaneous bilirubin) value was calculated.ResultsIn this study, there were no significant differences in maternal demographic characteristics, neonatal status and macronutrients in breast milk between the two groups (p > 0.05). There are differences in the structure of intestinal microbiota between LBMJ and the control group. At the genus level, the relative abundance of Klebsiella in the case group is high (p < 0.05). At the same time, correlation analysis indicates that the abundance of Klebsiella is positively correlated with TcB value. The intestinal microbiota richness and diversity (Alpha diversity and Beta diversity) of the two groups were significantly different (p < 0.05). LEfSe analysis showed that 25 genera including Klebsiella was significantly enriched in the LBMJ infants, and the other 17 species are enriched in the control group. Functional prediction analysis indicated that 42 metabolic pathways may be related to the occurrence of LBMJ.ConclusionIn conclusion, characteristic changes are seen in intestinal microbiota compositions between LBMJ infants and the healthy controls. Klebsiella is closely associated with the severity of the disease, which may be due to enhanced β-glucuronidase activity.

Beneficial changes in gut microbiota after phototherapy for neonatal hyperbilirubinemia

“…These data indicate that the gut microbiota are important participants in the bilirubin metabolism. An increase in diversity and abundance is a common manifestation of gut microbiota disorders in NH (17,18). The present study found that 24 h of phototherapy markedly reduced the diversity and abundance of intestinal flora, suggesting that phototherapy may also partially reverse the microflora changes caused by NH.…”

“…S. salivarius can release bacteriocins (salivary A5 and B) (23-25), thereby antagonizing opportunistic or pathogenic bacteria. As aforementioned, the diversity and abundance of the gut microbiota in newborns with hyperbilirubinemia are increased and contain a number of opportunistic pathogens (17,18). Therefore, the increase in the abundance of S. salivarius after phototherapy may partially improve intestinal defense function.…”

Breast Milk Jaundice

“…Кроме того, младенцы сравниваемых групп различались по уровням глицериновой и янтарной кислот, а также фенилаланина [31]. Дети с желтухой грудного молока отличаются от детей без желтухи альфа-и бетаразнообразием микробиоты, и в частности большим количеством микробов рода Klebsiella, что, по мнению авторов исследования, ведет к увеличению активности ␤-глюкуронидазы в кишечнике и таким образом способствует развитию желтухи грудного молока [40]. Следует учитывать, что в последнее исследование включены дети с поздней манифестацией желтухи грудного молока, то есть особенности микробиоты могли быть частично обусловлены возрастом детей.…”