Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan Plateau

Abstract

Strain Y74T was an isolate from the sandy soil in the town of Huatugou, Qinghai–Tibet Plateau, China. An analysis of this strain's phenotypic, chemotaxonomic, and genomic characteristics established the relationship of the isolate with the genus Planococcus. Strain Y74T was able to grow between 4 and 42°C (with an optimum temperature of 28°C) at pH values of 6–8.5 and in 0%–7% (w/v) NaCl. The dominant quinones were MK‐8 and MK‐7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The majority of the fatty acid content was anteiso‐C15:0 (28.8%) followed by C16:1 ω7c alcohol (20.9%) and iso‐C14:0 (13.4%). The 16S rRNA gene sequence similarity analysis demonstrated a stable branch formed by strain Y74T and Planococcus halotolerans SCU63T (99.66%). The digital DNA–DNA hybridization between these two strains was 57.2%. The G + C content in the DNA of Y74T was 44.5 mol%. In addition, the morphological, physiological, and chemotaxonomic pattern clearly differentiated the isolates from their known relatives. In conclusion, the strain Y74T (=JCM 32826T = CICC24461T) represents a novel member of the genus Planococcus, for which the name Planococcus antioxidans sp. nov. is proposed. Strain Y74T was found to have potent antioxidant activity via its hydrogen peroxide tolerance and its 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical‐scavenging activity. The DPPH radical‐scavenging activity was determined to be 40.2 ± 0.7%. The genomic analysis indicated that six peroxidases genes, one superoxide dismutase gene, and one dprA (DNA‐protecting protein) are present in the genome of Y74T.

Streptomyces gobiensis sp. nov., an antimicrobial producing actinobacterium isolated from soil under black Gobi rocks

“…Genomic DNA extraction was performed as described previously [19]. The full-length 16S rRNA gene sequence was extracted from the genome annotated in the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) [20][21][22] and deposited as MZ497413.1 in the GenBank/EMBL/DDBJ databases.…”

Mesobacillus harenae sp. nov., isolated from the sandy soil of a cold desert

“…We compared the 16S rRNA gene sequence of strain Y40 T with those of type strains in the EzTaxon database [16] ( Mesobacillus zeae JJ-247 T , Mesobacillus foraminis CV53 T , Mesobacillus maritimus KS16-9 T , Neobacillus soli NBRC 102451 T , Cytobacillus gottheilii WCC 4585 T and Neobacillus vireti LMG 21834 T ) and the similarities were 98.0, 97.7, 97.4, 97.1, 97.1 and 97.1 %, respectively. The highly related 16S rRNA gene sequences of the type strains from the genera Nesobacillus , Peribacillus , Neobacillus , Cytobacillus and Bacillus were chosen for phylogenetic analysis, along with Bacillus subtilis DSM10 T which was used as an outgroup.…”

“…The Qaidam Basin is located in the northeast Qinghai-Tibet Plateau, which is a very harsh environment with an arid climate and high solar radiation. These special characteristics have resulted in a large number of new untapped microbial resources in the region [14][15][16].…”

Whole genome sequencing and comparative genomic analyses of Planococcus alpniumensis MSAK28401T, a new species isolated from Antarctic krill

“…These changes indicated that genus Planococcus and Planomicrobium have a close phylogenetic relationship. Usually, the main splitting points of the 16S rRNA sequence between the genera Planococcus and Planomicrobium were located at sites 183 and 190 ( E. coli counting), which in the Planococcus are T and A, whereas in the Planomicrobium are C and G [ 8 ].…”

“…Planococcus has the following known features: Gram-positive, multicellular morphology (cocci, short rod, or rod), aerobic, and no sporulation [ 8 ]. Representative strains of genus Planococcus usually grow in cold and/or saline-alkali soil with high salt concentrations, e.g., Arctic, Antarctic, and marine environments [ 9 – 11 ].…”