Characteristics of Pediococcus pentosaceus Q6 isolated from Elymus nutans growing on the Tibetan Plateau and its application for silage preparation at low temperature

Abstract

The selection of P. pentosaceus inoculants for improving silage quality at low temperature, which provides a candidate strain to make high-quality silage in regions with frigid climate.

Microbial Community Dynamics and Natural Fermentation Profiles of Ensiled Alpine Grass Elymus nutans Prepared From Different Regions of the Qinghai-Tibetan Plateau

“…The dominant bacteria in the fresh grasses from TZ, GL, DX, and NQ were Pseudomonas, Lactobacillu, W. cibaria, and L. mesenteroides, respectively, which was also different from previous studies on fresh whole crop corn, grass, and legume forages (Ni et al, 2017;Guan et al, 2018;Liu et al, 2019;Xu et al, 2019;Yan et al, 2019). Moreover, the LAB composition in E. nutans silage is totally different that in whole crop corn silage (Xu et al, 2019). Results from the present study showed that the bacteria were mainly comprised by P. pentosaceus, Lactobacillus sp., L. mesenteroides, and L. coryniformis after 60 days of fermentation in the silage samples from TZ, while L. brevis was the dominant species in the silage samples from DX after the 14, 60, and 90 days fermentation.…”

“…Therefore, the LAB succession at different fermentation stages of E. nutans was also significantly different. The dominant bacteria in the fresh grasses from TZ, GL, DX, and NQ were Pseudomonas, Lactobacillu, W. cibaria, and L. mesenteroides, respectively, which was also different from previous studies on fresh whole crop corn, grass, and legume forages (Ni et al, 2017;Guan et al, 2018;Liu et al, 2019;Xu et al, 2019;Yan et al, 2019). Moreover, the LAB composition in E. nutans silage is totally different that in whole crop corn silage (Xu et al, 2019).…”

“…Due to the extremely harsh environment on the plateau, forage yields in the alpine grassland are normally quite low (Thomas et al, 2016). Additionally, the increased number of domestic animals and overgrazing in the past decades even aggravated the feed shortage (Xu et al, 2019). Therefore, efficient forage preservation is considered as an important farming strategy to alleviate feed shortage of the traditional year-round grazing system on the QTP.…”

“…However, little information, to our best knowledge, is available on the epiphytic microbial community of forages from the grassland in different areas of the QTP, and its subsequent influences on silage fermentation and quality of native grasses. As a typical and important alpine forage grass, Elymus nutans is widely distributed in the QTP with high nutritional value and palatability for livestock (Chen et al, 2013;Xu et al, 2019).…”

Pediococcus pentosaceus, a future additive or probiotic candidate

“…As a major advance in the application of animal husbandry, P. pentosaceus DSM 16,244 was certified as safe additive for all animal species by the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) [67]. With regard to crops, Elymus nutansderived P. pentosaceus Q6 was beneficial for the storage quality of Elymus nutans silages at low temperature [16]. Thus, P. pentosaceus Q6 might be a cold-resistant candidate for high-quality silage storage.…”