Characteristics of
            <i>Helicobacter pylori</i>
            Infection in Jamaican Adults with Gastrointestinal Symptoms

Hisada, Michie; Lee, Michael G.; Hanchard, B; Owens, Marilyn; Song, Qunsheng; Doorn, Leen–Jan van; Cutler, Alan F.; Gold, Benjamin D.

doi:10.1128/jcm.39.1.212-216.2001

Cited by 13 publications

(14 citation statements)

References 26 publications

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“…Our study had two major limitations. First, although our serologic assay was validated and optimized for use in Jamaican adults and children [12,13], some misclassifications of infection status may have occurred because serologic assessment alone is not sufficient for distinguishing active infection from recent past infection. Any misclassification of H. pylori status would be unrelated to genotype, resulting in estimates biased towards the null.…”

Section: Discussionmentioning

confidence: 99%

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Polymorphisms in Cytokine Genes and Risk of Helicobacter pylori Infection among Jamaican Children

et al. 2006

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Section: Discussionmentioning

confidence: 99%

“…A previously validated enzyme-linked immunosorbent assay (ELISA) detected anti -H. pylori IgG antibodies in plasma [12]. Individuals were considered H. pylori seropositive if the ELISA optical density (OD) was > 1.3, seronegative if OD ≤ 0.9, and seroindeterminate if 0.9 < OD ≤ 1.3.…”

Section: Laboratory Methodsmentioning

confidence: 99%

Polymorphisms in Cytokine Genes and Risk of Helicobacter pylori Infection among Jamaican Children

et al. 2006

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“…That nearly all (95.8%) patients were H. pylori positive (and carried cagA-positive strains) is typical for a preindustrial society (5,15,23). Our collection of strains, with multiple isolates from the same individuals, should be particularly useful in evaluating host adaptations since the isolates originated in this nonacculturated population.…”

Section: Discussionmentioning

confidence: 99%

Responses of Endoscopy Patients in Ladakh, India, to Helicobacter pylori Whole-Cell and CagA Antigens

Romero–Gallo

Pérez-Pérez

Novick

et al. 2002

Clin Vaccine Immunol

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Although Helicobacter pylori is a cosmopolitan colonizer of the human stomach, the responses among persons in remote populations from whom H. pylori was cultured have not been studied. We report on studies of 189 persons in the Ladakh region of India in whom serum immunoglobulin G responses to H. pylori whole-cell and CagA antigens were measured. H. pylori was isolated from 68 of these patients. An H. pylori whole-cell antigen derived from Ladakhi strains outperformed a similar antigen from U.S. strains, as determined by antigenspecific enzyme-linked immunosorbent assays. In total, 95% of the population was seropositive, including individuals responding only to the CagA antigen. Correlation with culture results showed that these were true positives and, therefore, that the H. pylori whole-cell serology was falsely negative in some cases. In addition to establishing a collection of H. pylori isolates from a remote area in the world, we show that use of H. pylori whole-cell and CagA serology together increases the sensitivity for the detection of colonization.Although humans in all parts of the world may be colonized with Helicobacter pylori (2, 11), most detailed studies correlating serology with bacterial culture have been conducted in industrialized countries or in urban centers in developing countries (5, 23). In part, this pattern stems from the relative difficulty of acquiring proper specimens from indigenous peoples in remote areas, where much of the infrastructure that permits clinical investigation and subsequent biomedical research is lacking (4).Ladakh, India, is a remote trans-Himalayan region that is sparsely populated, owing to its high elevation (Ն3,500 m) and lack of rainfall. Its population of about 220,000 consists of individuals of Tibetan-Buddhist ethnicity (about 70%) and of Muslims originating in the Baltistan area of Pakistan and other areas of India (about 30%). The majority of the Muslim subjects (Argons) are the descendants of Kashmiri Muslim fathers and Ladakhi Buddhist mothers. Anecdotally, residents of Leh, the capitol of Ladakh, have a high incidence of upper gastrointestinal (GI) tract complaints, and before the advent of antacids and histamine receptor-blocking agents, they were believed to have had a high incidence of peptic ulcers and upper GI tract bleeding. Given that most individuals in such an environment would be expected to carry H. pylori (11), we conducted field studies of H. pylori carriage and serology in Ladakh.In an initial serological survey, performed during the summer of 1995, approximately 380 randomly selected individuals were screened for H. pylori antibodies by using a serological field test kit (Quidel, Inc., San Diego, Calif.). Included were residents of small rural communities and of Leh, including Tibetan orphans. The results of this survey were that about 95% of Ladakhis were H. pylori seropositive whether or not they had any potentially relevant symptoms referable to the upper GI tract (S. Wall, N. Shah, and R. P. Novick, unpublished data).On the basis of ...

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“…This assay has been validated using serum specimens from adult and paediatric patients from North and South America, Alaska, Africa and the Caribbean who have had both upper endoscopy with biopsy and urea breath test [17][18][19]. In a research setting such as this study, the ELISA demonstrates a sensitivity and specificity of >92%, respectively, and positive and negative predictive values of >93%.…”

Section: Heliobacter Pylorimentioning

confidence: 90%

“…In a nested case-control study, one vial of frozen plasma was selected from the biospecimen repository for each upper gastrointestinal bleeding case and an equal number of matched controls for detection of H. pylori IgG antibodies, using a previously reported, research-based, well-validated enzyme immunoassay with whole cell proteins from an H. pylori isolate [15,16]. This assay has been validated using serum specimens from adult and paediatric patients from North and South America, Alaska, Africa and the Caribbean who have had both upper endoscopy with biopsy and urea breath test [17][18][19]. In a research setting such as this study, the ELISA demonstrates a sensitivity and specificity of >92%, respectively, and positive and negative predictive values of >93%.…”

Section: Heliobacter Pylorimentioning

confidence: 99%

Upper gastrointestinal bleeding in haemophiliacs: incidence and relation to use of non‐steroidal anti‐inflammatory drugs

et al. 2007

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This multicentre study sought to estimate the incidence of upper gastrointestinal (UGI) bleeding in haemophiliacs and its relationship to use of non-steroidal anti-inflammatory drugs (NSAIDs). Cox models were used to estimate relative hazards (RH) with 95% confidence intervals (CI) for postulated risk factors. Conditional logistic regression and stored sera were used to assess UGI bleeding risk with Heliobacter pylori seropositivity in cases compared with closely matched controls. During a mean of 17.4 months (range 2-34), 2285 participants, ages 13-89 (mean 36.5) were followed for 3309 person-years (py). Forty-two experienced a UGI bleeding event (incidence 1.3 per 100 py), most from ulcer (11), gastritis (four), varices (five) and Mallory Weiss tears (eight). RH was significantly increased with traditional NSAID use for <1 month (OR: 3.66; 95% CI: 1.1-11.9), but not with coxibs use. RH was significantly and independently increased with age >46 years (3.5; 95% CI: 1.1-10.6) and hepatic decompensation (4.4; 95% CI: 1.7-11.6). Likelihood of bleeding was substantially but not significantly increased (OR: 4.6; 95% CI: 0.3-83.9) with H. pylori seropositivity. These findings suggest that coxibs are a safer alternative than traditional NSAIDs in the treatment of haemophilic arthropathy.

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Characteristics of Helicobacter pylori Infection in Jamaican Adults with Gastrointestinal Symptoms

Cited by 13 publications

References 26 publications

Polymorphisms in Cytokine Genes and Risk of Helicobacter pylori Infection among Jamaican Children

Polymorphisms in Cytokine Genes and Risk of Helicobacter pylori Infection among Jamaican Children

Responses of Endoscopy Patients in Ladakh, India, to Helicobacter pylori Whole-Cell and CagA Antigens

Upper gastrointestinal bleeding in haemophiliacs: incidence and relation to use of non‐steroidal anti‐inflammatory drugs

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