Characteristics of Human Turbinate-Derived Mesenchymal Stem Cells Are Not Affected by Allergic Condition of Donor

Abstract

The characteristics of mesenchymal stem cells (MSCs) derived from human turbinates (hTMSCs) have not been investigated in allergic rhinitis. We evaluated the influence of allergic state of the donor on the characteristics, proliferation, and differentiation potential of hTMSCs, compared with hTMSCs derived from non-allergic patients. hTMSCs were isolated from five non-allergic and five allergic patients. The expression of toll-like receptors (TLRs) in hTMSCs was measured by FACS, and cell proliferation was measured using a cell counting kit. Cytokine secretion was analyzed using multiplex immunoassays. The osteogenic, chondrogenic, and adipogenic differentiation potentials of hTMSCs were evaluated by histology and gene expression analysis. In allergic patients, FACS analysis showed that TLR3 and TLR4 were more highly expressed on the surface of hTMSCs than TLR2 and TLR5. The proliferation of hTMSCs was not influenced by the presence of TLR priming. The expression of IL-6, IL-8, IL-12, IP-10, and RANTES was upregulated after the TLR4 priming. The differentiation potential of hTMSCs was not influenced by TLR priming. These characteristics of hTMSCs were similar to those of hTMSCs from non-allergic patients. We conclude that the allergic condition of the donor does not influence TLR expression, proliferation, or immunomodulatory potential of hTMSCs.

Is mesenchymal stem cell effective for allergic rhinitis? A protocol for a systematic review and meta-analysis

“…The immune imbalance between Th1 and Th2 cells is the immunological basis of the incidence of AR, that is, the incidence of AR is mainly the advantage of Th2 cell immune response, 30 which leads to a series of nasal allergy symptoms by mediating the release of a variety of inflammatory mediators and cytokines. 31 Th2 cells initiate and maintain inflammation in allergic immune response by releasing large amounts of Th2 cytokines (interleukin (IL)-4, IL-5, IL-13, etc).…”

“…Therefore, regulating Th1/Th2 immune balance may play an important role in the treatment of allergic inflammation, which is also an important entry point in treatment. 30 Genç et al 33 co-cultured pulp-derived MSC (DFSC) with Peripheral blood mononuclear cell (PBMC) of patients with asthma, measured the proliferation of CD4+ T lymphocytes by carboxyfluorescein diacetate succinimidyl ester (CFSE) staining combined with flow cytometry, and found that DFSC could inhibit the proliferation of CD4+ T lymphocytes. The relative expression levels of IL-4 and GATA-3 mRNA were decreased, the relative expression levels of interferon-γ, T-bet and IL-10 were increased, and the percentage of Tregs was increased.…”

Evaluation of characteristic of human turbinate derived mesenchymal stem cells cultured in the serum free media

“…In addition, it was proved that the hTMSCs exhibited approximately 5 times higher proliferation than the hBMSCs [ 15 ]. 3 Additionally, it has been shown that the donor age do not influence the properties of hTMSCs, unlike other human MSCs [ 4 ] and local condition of nasal cavity including allergic state or nasal septal deviation also do not have the effect on the feature of them [ 16 , 17 ]. The excellent potency of hTMSCs compared to MSCs from other tissues could make our group pay particular attention to the cells.…”

Accelerated Bone Regeneration via Three-Dimensional Cell-Printed Constructs Containing Human Nasal Turbinate-Derived Stem Cells as a Clinically Applicable Therapy

“…The glass cover slide was removed, and cells attached to the culture plate were harvested using 0.25% trypsin in 1 mM EDTA solution. Similar to types of MSCs, hTMSCs have a high proliferative capacity and the ability to differentiate into multiple lineage cell types. ,,, hTMSCs were expanded in three to four passages for use in experiments. hBMSCs were obtained from the Catholic Institute of Cell Therapy (Catholic MASTER Cells, Seoul, Korea).…”